Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze

YILAR, Melih; Bayar, Yusuf

Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze

Melih YILAR, (Ahi Evran University, Faculty of Agriculture, Department of Plant Protection, 40200 Kırşehir, TURKEY)

Yusuf BAYAR (Ahi Evran University, Faculty of Agriculture, Department of Plant Protection, 40200 Kırşehir, TURKEY)

Türk Tarım ve Doğa Bilimleri Dergisi

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Yıl: 2018 Cilt: 5 Sayı: 2 Sayfa Aralığı: 121 - 126 Metin Dili: İngilizce İndeks Tarihi: 01-04-2019

Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze

Öz:

This study was carried out to determine the antifungal potential of Thymbra spicata L. and Rosmarinusofficinalis L. (Lamiaceae) plant essential oils against the Monilinia fructigena Honey in Whetze pathogen which isone of the major disease agents of soft seeds. The essential oils were dropped on a filter paper adhered to thecovers of petri dishes with a micropipette at the doses of 0 (Control), 0.5, 0.8, 1, 1.5, 2, 4, 8, 16 μl/petri dish. Atthe end of the seven days incubation period, the diameters of the mycelium growth in petri dishes weremeasured with an automatic caliper. As a result, T. spicata and R. officinalis plant essential oils were found to bestatistically significant on the pathogen. Depending on the increased dose, R. officinalis essential oil inhibited thedevelopment of the M. fructigena mycelium and inhibited by 100% in 16 μl/petri dish. On the other hand, T.spicata essential oil influenced the mycelium development of the pathogen more and inhibited it by 100% at thedose of 2μl/petri dish. As a result ofdose-response study regarding T. spicata and R. officinalis essential oilsagainst M. fructigena the LC50 and LC90 values were found to be 0.80-1.63 and 5.36-12.17, respectively. Thesefindings showed that T. spicata and R. officinalis essential oils may be an alternative to synthetic fungicides forcontrolling M. fructigena.

Anahtar Kelime:

Thymbra spicata L. ve Rosmarinus officinalis L. Uçucu Yağlarının Monilinia fructigena Honey in Whetze’ya Karşı Antifungal Aktivitesi

Öz:

Bu çalışma, yumuşak çekirdeklilerin önemli hastalık etmenlerden biri olan Monilinia fructigena patojenine karşı Thymbra spicata L. ve Rosmarinus officinalis L. (Lamiaceae) bitki uçucu yağlarının antifungal potansiyelinin belirlenmesi amacıyla yürütülmüştür. Uçucu yağlar petri kaplarının kapaklarına yapıştırılan filtre kağıdına 0 (Kontrol),0.5, 0.8, 1, 1.5, 2, 4, 8, 16 μl/petri dozda mikropipetle damlatılmıştır. Yedi günlük inkubasyon süresi sonunda petrilerdeki miselyum çapları otomatik kumpas ile ölçülmüştür. Sonuçta T. spicata ve R. officinalis bitki uçucu yağları patojen üzerine istatistiki olarak önemli derece etkili bulunmuştur. R. officinalis uçucu yağı artan doza bağlı olarak M. fructigena miselyum gelişimini engellemiş olup 16 μl/petri kabı dozda %100 engellemiştir. T. spicata uçucu yağı ise patojenin miselyum gelişimi üzerine daha fazla etki göstermiş; 2μl/petri kabı dozda %100 inhibe etmiştir. T. spicata ve R. officinalis uçucu yağlarının M. fructigenakarşı doz-etki çalışması sonucundaLC50 ve LC90 değerleri sırasıyla, 0.80-1.63 ve 5.36-12.17 olarak bulunmuştur. Bu bulgular, T. spicata ve R. officinalis uçucu yağlarının, M. fructigena kontrolünde sentetik fungusitlere alternatif olabileceğini göstermiştir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	YILAR M, Bayar Y (2018). Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze. , 121 - 126.
Chicago	YILAR Melih,Bayar Yusuf Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze. (2018): 121 - 126.
MLA	YILAR Melih,Bayar Yusuf Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze. , 2018, ss.121 - 126.
AMA	YILAR M,Bayar Y Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze. . 2018; 121 - 126.
Vancouver	YILAR M,Bayar Y Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze. . 2018; 121 - 126.
IEEE	YILAR M,Bayar Y "Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze." , ss.121 - 126, 2018.
ISNAD	YILAR, Melih - Bayar, Yusuf. "Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze". (2018), 121-126.

APA	YILAR M, Bayar Y (2018). Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze. Türk Tarım ve Doğa Bilimleri Dergisi, 5(2), 121 - 126.
Chicago	YILAR Melih,Bayar Yusuf Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze. Türk Tarım ve Doğa Bilimleri Dergisi 5, no.2 (2018): 121 - 126.
MLA	YILAR Melih,Bayar Yusuf Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze. Türk Tarım ve Doğa Bilimleri Dergisi, vol.5, no.2, 2018, ss.121 - 126.
AMA	YILAR M,Bayar Y Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze. Türk Tarım ve Doğa Bilimleri Dergisi. 2018; 5(2): 121 - 126.
Vancouver	YILAR M,Bayar Y Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze. Türk Tarım ve Doğa Bilimleri Dergisi. 2018; 5(2): 121 - 126.
IEEE	YILAR M,Bayar Y "Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze." Türk Tarım ve Doğa Bilimleri Dergisi, 5, ss.121 - 126, 2018.
ISNAD	YILAR, Melih - Bayar, Yusuf. "Antifungal Activity of Thymbra spicata L. and Rosmarinus officinalis L. Essential Oils against Monilinia fructigena Honey in Whetze". Türk Tarım ve Doğa Bilimleri Dergisi 5/2 (2018), 121-126.