Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia

güneş, ışın; Arslan, Mustafa; ARPACI, Ayşe Hande; DİKMEN, Bayazıt; KALAYCI, Dilek; BEŞKARDEŞ, Elif; kurtipek, ömer; ÇOMU, Faruk Metin

Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia

Dilek KALAYCI, (Department of Anaesthesiology and Reanimation, Abdurrahman Yurtaslan Oncology Training and Research Hospital, Ankara, Turkey)

Ayşe Hande ARPACI, (Department of Oral and Maxillofacial Surgery, Ankara University Faculty of Dentistry (Anaesthesiology Reanimation specialist) Besevler, Ankara, Turkey)

Faruk METİN ÇOMU, (Kırıkkale University, Faculty of Medicine, Department of Physiology, Kırıkkale, Turkey)

Işın GÜNEŞ, (Erciyes Üniversitesi, Tıp Fakültesi, Anesteziyoloji ve Reanimasyon Anabilim Dalı, Kayseri, Türkiye)

Elif BEŞKARDEŞ, (Department of Anaesthesiology and Reanimation, Gazi University Medical Faculty, Ankara, Turkey)

Ömer KURTİPEK, (Department of Anaesthesiology and Reanimation, Gazi University Medical Faculty, Ankara, Turkey)

Mustafa ARSLAN, (Department of Anaesthesiology and Reanimation, Gazi University Medical Faculty, Ankara, Turkey)

Bayazıt DİKMEN (Department of Anaesthesiology and Reanimation, Gazi University Medical Faculty, Ankara, Turkey)

Gazi Medical Journal

3 1

Yıl: 2018 Cilt: 29 Sayı: 1 Sayfa Aralığı: 8 - 11 Metin Dili: İngilizce İndeks Tarihi: 05-04-2019

Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia

Öz:

Aim: Micro and macrovascular complications due to long-term hyperglycemiaare associated with increased mortality and morbidity. Erythrocytes exposedto hyperglycemia for a long time may cause morphological changes inerythrocytes such as decreased deformability and development ofaggregation. As a result, complications such as shortening life span oferythrocytes, impairment of oxygen carrying capacity, tissue hypoxia mayoccur. In our study, we would like to investigate the effects of Sevoflurane andDesflurane on erythrocyte deformability during transient hyperglycemia.Materials and Methods: In this study, 30 male Wistar albino rats were used.The animals were randomly divided into five groups, each contained 6 rats:Diabetic control (group DC), diabetic hyperglycemia group (group DH),diabetic hyperglycemia group with desflurane (group DH-D), and diabetichyperglycemia group with sevoflurane (group DH-S) groups. Another 6 ratswithout diabetes were assigned as control group (group C). Streptozotocin-induced diabetic rats were kept 6 weeks, then transient hyperglycemia wascreated, and the administration of sevoflurane and desflurane wereperformed. After 24 hours blood samples were obtained and deformabilitymeasurements were performed in erythrocyte suspensions containing Htc5% in a PBS buffer.Results: Diabetes mellitus was found to increase relative resistance in thecontrol group (p <0.0001). Acute hyperglycemia increased relative resistancein diabetes control, relatively. Group DH, Group DH-D and Group DH-Sdeformability index were significantly different when compared to Group DC(p=0.007, p=0.025, p=0.016, respectively). It was found that administration ofdesflurane or sevoflurane did not alter erythrocyte deformability duringacute hyperglycemia (p = 0.591, p = 0.739).Conclusion: As a consequence, we think that we can safely use inhalationanesthetics such as Desflurane and Sevoflurane during acute hyperglycemiaattacks. But, it needs further investigation as both experimental and clinical

Anahtar Kelime:

Geçici Hiperglisemi anında Sevofluran ve Desfluranın Eritrosit Deformabilitesi Üzerine Etkilerinin Araştırılması

Öz:

Amaç: Uzun süreli hipergliseminin oluşturduğu mikro ve makrovasküler komplikasyonlar mortalite ve morbidite artışı ile birliktedir. Eritrositlerin ise yaşam süreleri boyunca uzun süre hiperglisemiye maruz kalmaları morfolojik olarak eritrositlerde deformabilitede azalma ve agregasyon gelişmesi gibi bir takım değişikliklere neden olmaktadır. Bunun sonucunda eritrositlerin yaşam sürelerinde kısalma, oksijen taşıma kapasitesinde bozukluk, doku hipoksisi gibi komplikasyonlar meydana gelmektedir. Sevofluran ve desfluranın ise eritrosit deformabilitesi üzerine etkileri ile ilgili çeşitli çalışmalar mevcuttur. Biz de bu çalışmamızda geçici olarak oluşturulan hiperglisemi sırasında sevofluran ve desfluranın eritrosit deformabilitesi üzerine etkilerini araştırmayı amaçladık. Yöntem: Çalışma Gazi Üniversitesi Deneysel ve Klinik Araştırma Merkezi'nde Gazi Üniversitesi Deney Hayvanları Etik Kurulu onayı ile yapıldı. Çalışmaya 30 rat dahil edildi. Ratlar; kontrol grubu, diyabetik-kontrol, diyabetik hiperglisemi, diyabetik–hiperglisemi-sevofluran, diyabetik-hiperglisemi- desfluran olmak üzere 5 gruba ayrıldı. Streptozosin ile diyabet oluşturulan ratlar 6 hafta yaşatıldıktan sonra geçici hipergilisemi oluşturuldu ve sevofluran ve desfluran anestezisi uygulandı. 24 saat sonra kan örnekleri alındı ve santrifüj edildi. Eritrosit deformabilitesi sabit akım filtrometre sistemleri kullanılarak ölçüldü. Rölatif rezistansının artması eritrosit deformabilitesinin azalması olarak yorumlandı. Bulgular: Diyabet oluşturulmasının kontrol grubuna göre rölatif rezistansı arttırdığı bulundu (p<0.0001). Akut hiperglisemi, diyabet kontrol grubuna göre rölatif rezistansı arttırdı. Grup DH, Grup DH-D ve Grup DH-S deformabilite indeksi Grup DK ile karşılaştırıldığında anlamlı olarak farklı bulundu (p=0.007, p=0.025, p=0.016). Akut hiperglisemi sırasında Desfluran veya Sevofluran uygulanmasının ise eritrosit deformabilitesini değiştirmediği tespit edildi (p=0.591, p=0.739). Sonuç: Sevofluran ve Desfluran gibi inhalasyon ajanların akut hiperglisemi sırasında güvenle kullanılabileceğini düşünmekteyiz. Ancak bu bulguların daha detaylı ve geniş serilerde yapılacak klinik ve deneysel çalışmalarla desteklenmesi gerekmektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KALAYCI D, ARPACI A, ÇOMU F, güneş ı, BEŞKARDEŞ E, kurtipek ö, Arslan M, DİKMEN B (2018). Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia. , 8 - 11.
Chicago	KALAYCI Dilek,ARPACI Ayşe Hande,ÇOMU Faruk Metin,güneş ışın,BEŞKARDEŞ Elif,kurtipek ömer,Arslan Mustafa,DİKMEN Bayazıt Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia. (2018): 8 - 11.
MLA	KALAYCI Dilek,ARPACI Ayşe Hande,ÇOMU Faruk Metin,güneş ışın,BEŞKARDEŞ Elif,kurtipek ömer,Arslan Mustafa,DİKMEN Bayazıt Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia. , 2018, ss.8 - 11.
AMA	KALAYCI D,ARPACI A,ÇOMU F,güneş ı,BEŞKARDEŞ E,kurtipek ö,Arslan M,DİKMEN B Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia. . 2018; 8 - 11.
Vancouver	KALAYCI D,ARPACI A,ÇOMU F,güneş ı,BEŞKARDEŞ E,kurtipek ö,Arslan M,DİKMEN B Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia. . 2018; 8 - 11.
IEEE	KALAYCI D,ARPACI A,ÇOMU F,güneş ı,BEŞKARDEŞ E,kurtipek ö,Arslan M,DİKMEN B "Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia." , ss.8 - 11, 2018.
ISNAD	KALAYCI, Dilek vd. "Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia". (2018), 8-11.

APA	KALAYCI D, ARPACI A, ÇOMU F, güneş ı, BEŞKARDEŞ E, kurtipek ö, Arslan M, DİKMEN B (2018). Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia. Gazi Medical Journal, 29(1), 8 - 11.
Chicago	KALAYCI Dilek,ARPACI Ayşe Hande,ÇOMU Faruk Metin,güneş ışın,BEŞKARDEŞ Elif,kurtipek ömer,Arslan Mustafa,DİKMEN Bayazıt Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia. Gazi Medical Journal 29, no.1 (2018): 8 - 11.
MLA	KALAYCI Dilek,ARPACI Ayşe Hande,ÇOMU Faruk Metin,güneş ışın,BEŞKARDEŞ Elif,kurtipek ömer,Arslan Mustafa,DİKMEN Bayazıt Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia. Gazi Medical Journal, vol.29, no.1, 2018, ss.8 - 11.
AMA	KALAYCI D,ARPACI A,ÇOMU F,güneş ı,BEŞKARDEŞ E,kurtipek ö,Arslan M,DİKMEN B Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia. Gazi Medical Journal. 2018; 29(1): 8 - 11.
Vancouver	KALAYCI D,ARPACI A,ÇOMU F,güneş ı,BEŞKARDEŞ E,kurtipek ö,Arslan M,DİKMEN B Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia. Gazi Medical Journal. 2018; 29(1): 8 - 11.
IEEE	KALAYCI D,ARPACI A,ÇOMU F,güneş ı,BEŞKARDEŞ E,kurtipek ö,Arslan M,DİKMEN B "Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia." Gazi Medical Journal, 29, ss.8 - 11, 2018.
ISNAD	KALAYCI, Dilek vd. "Investigation of the Effects of Sevoflurane and Desflurane on Erythrocyte Deformability in Transient Hyperglycemia". Gazi Medical Journal 29/1 (2018), 8-11.