The snow drop has a wide distribution of our country is an important plant in terms of species diversity and endemic richness. Due to widespread use as ornamental plant, it has commercial economic value. For this reason, sustainable production of snowdrop bulbs is needed using biotechnological methods. In this study, it was aimed to find the optimum and efficient conditions of snowdrop bulb in vitro using different plant growth regulators and activated charcoal. For this purpose, BAP, NAA, GA3 and 2,4-D plant growth regulators combinations and active charcoal at different concentrations were used in MS medium. While bulblet number were found 3.67 in MS medium containing 1 mg l-1 BAP and 0.1 mg l-1 NAA among applied hormone combination, the highest bulblet number per explant as 5.95 were observed on MS medium containing and 5 g l-1 activated charcoal. In vitro rooting of bulblets used MS medium supplemented with 1 g l-1 and 5 g l-1 activated charcoal and then acclimatization of bulblet were done successfully. Consequently, the result from the experiment, activated charcoal promoted the highest bulblet multiplication in vitro. Among the hormone combinations applied, 3.67 shallots were obtained in MS medium containing 1 mg l-1 BAP and 0.1 mg l-1 NAA.