In this study, I. tinctoria (woad) cultivated under controlled conditions was investigated for its tolerance to environmental stress conditions and its response to external polyamines. The plants were exposed to salinity stress by irrigating the soil with four different concentrations (0, 50, 100 and 200 mM) of NaCl solution While plants generally tolerated increasing salt doses, they were affected by a salt concentration of 200 mM and the yield, including indigo, decreased. As a second step, seedlings were exposed to drought stress and the effects of drought on the 3rd, 7th and 10th days were examined. While the number of days in the drought increased, the indigo and yield decreased, and the lowest values were obtained at 10th day. In the third step, spermine, spermidine and putresin polyamines were applied at four different concentrations (0, 0.1, 1 and 2 mM) on the leaves of plant. In practice, all polyamines increased the yield of indigo and seedlings, however the best results were obtained from putresin. The fourth stage was polyamine + salt stress application and in this stage, putresin was the most effective polyamine which increased the amount of indigo and leaf yield. Polyamine + drought stress was the fifth stage, and all polyamines, especially spermine, were found to be effective in this stage.