Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity

Gürgen, Seren Gülşen; SEYHAN, Sermin; ÖZOĞUL, Candan; HIRFANOĞLU, İbrahim Murat; GOKTAS, GULESER; ERDOĞAN, Deniz; Saribas, Gulistan Sanem; COŞKUN AKÇAY, NESLİHAN; Akyol, Seda

Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity

Sermin SEYHAN, (Gazi University Faculty of Medicine, Department of Histology and Embryology, Ankara, Turkey)

GÜLİSTAN SANEM SARIBAŞ, (Ahi Evran University Faculty of Medicine, Department of Histology and Embryology, Kırsehir, Turkey)

Neslihan COŞKUN AKÇAY, (Hacettepe University Faculty of Medicine, Department of Obstetrics and Gynecology, Ankara, Turkey)

SEREN GÜLŞEN GÜRGEN, (Celal Bayar University School of Vocational Health Services, Department of Histology and Embryology, Manisa, Turkey)

Seda Nur AKYOL, (Zekai Tahir Burak Women’s Health Education and Research Hospital, IVF operating room, Ankara, Turkey)

GÜLESER GÖKTAŞ, (Baskent University Faculty of Medicine, Department of Histology and Embryology, Ankara, Turkey)

İbrahim Murat HIRFANOĞLU, (Faculty of Medicine, Department of Pediatrics, Neonatology Unit, Gazi University, Ankara, Turkey)

Deniz ERDOĞAN, (Gazi University Faculty of Medicine, Department of Histology and Embryology, Ankara, Turkey)

Candan ÖZOĞUL (Gazi University Faculty of Medicine, Department of Histology and Embryology, Ankara, Turkey)

Gazi Medical Journal

3 1

Yıl: 2018 Cilt: 29 Sayı: 4 Sayfa Aralığı: 294 - 298 Metin Dili: İngilizce İndeks Tarihi: 11-04-2019

Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity

Öz:

Objective: The aim of the study was to investigate the prophylactic effects ofacetyl L-carnitine against to uterus induced by cisplatin.Methods: Twenty-four female Wistar albino rats were divided into four groups:group I (control) was administered with saline; group II was administered withacetyl L-carnitine; group III was administered with cisplatin; group IV waspretreated with acetyl L-carnitine before cisplatin intraperitoneal injection. After72h of cisplatin injection uterine tissue was removed. Histological andimmunohistochemical investigations were performed, respectively.Results: We found that the number of TUNEL and caspases positive cells wereincreased in the endometrial epithelium, subepithelial connective tissue,endometrial glands and stroma in group III compare to the other groups.Furthermore inflammation and edema were observed in uterus of rats in groupIII.Conclusion: We can concluded that pretreatment of acetyl L-carnitineadministration has protective effect on histological alteration of uterus causedby cisplatin.

Anahtar Kelime:

Sisplatin ile İndüklenmiş Uterus Toksisitesinde Asetil L-Karnitinin Koruyucu Etkisinin Araştırılması

Öz:

Amaç: Bu çalışmanın amacı, asetil L-karnitinin, sisplatinin neden olduğu uterus hasarına karşı profilaktik etkilerini araştırmaktır. Yöntem: Yirmi dört adet Wistar albino cinsi dişi sıçanlar dört gruba ayrıldı: 1. gruba (kontrol) salin uygulaması yapıldı; 2. gruba asetil L-karnitin uygulaması yapıldı; 3. gruba sisplatin uygulaması yapıldı; 4. grup ise sisplatinin intraperitoneal enjeksiyonu öncesinde asetil L-karnitin ile ön tedaviye tabi tutuldu. Sisplatin enjeksiyonundan 72 saat sonra uterus dokusu çıkarıldı. Histolojik ve immünohistokimyasal incelemeler yapıldı. Bulgular: TUNEL ve kaspaz pozitif hücre sayılarının, diğer gruplara göre 3. grupta endometriyal epitelde, subepitelyal bağ dokusunda, endometriyal bezlerde ve stromada arttığını bulduk. Ayrıca 3. Gruptaki ratların uterusunda inflamasyon ve ödem gözlenmiştir. Sonuç: Asetil L-karnitin ön tedavi uygulamasının, sisplatin kaynaklı uterus histolojisinin değişikliği üzerinde koruyucu etkisi olduğu sonucuna vardık.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SEYHAN S, Saribas G, COŞKUN AKÇAY N, Gürgen S, Akyol S, GOKTAS G, HIRFANOĞLU İ, ERDOĞAN D, ÖZOĞUL C (2018). Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity. , 294 - 298.
Chicago	SEYHAN Sermin,Saribas Gulistan Sanem,COŞKUN AKÇAY NESLİHAN,Gürgen Seren Gülşen,Akyol Seda,GOKTAS GULESER,HIRFANOĞLU İbrahim Murat,ERDOĞAN Deniz,ÖZOĞUL Candan Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity. (2018): 294 - 298.
MLA	SEYHAN Sermin,Saribas Gulistan Sanem,COŞKUN AKÇAY NESLİHAN,Gürgen Seren Gülşen,Akyol Seda,GOKTAS GULESER,HIRFANOĞLU İbrahim Murat,ERDOĞAN Deniz,ÖZOĞUL Candan Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity. , 2018, ss.294 - 298.
AMA	SEYHAN S,Saribas G,COŞKUN AKÇAY N,Gürgen S,Akyol S,GOKTAS G,HIRFANOĞLU İ,ERDOĞAN D,ÖZOĞUL C Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity. . 2018; 294 - 298.
Vancouver	SEYHAN S,Saribas G,COŞKUN AKÇAY N,Gürgen S,Akyol S,GOKTAS G,HIRFANOĞLU İ,ERDOĞAN D,ÖZOĞUL C Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity. . 2018; 294 - 298.
IEEE	SEYHAN S,Saribas G,COŞKUN AKÇAY N,Gürgen S,Akyol S,GOKTAS G,HIRFANOĞLU İ,ERDOĞAN D,ÖZOĞUL C "Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity." , ss.294 - 298, 2018.
ISNAD	SEYHAN, Sermin vd. "Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity". (2018), 294-298.

APA	SEYHAN S, Saribas G, COŞKUN AKÇAY N, Gürgen S, Akyol S, GOKTAS G, HIRFANOĞLU İ, ERDOĞAN D, ÖZOĞUL C (2018). Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity. Gazi Medical Journal, 29(4), 294 - 298.
Chicago	SEYHAN Sermin,Saribas Gulistan Sanem,COŞKUN AKÇAY NESLİHAN,Gürgen Seren Gülşen,Akyol Seda,GOKTAS GULESER,HIRFANOĞLU İbrahim Murat,ERDOĞAN Deniz,ÖZOĞUL Candan Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity. Gazi Medical Journal 29, no.4 (2018): 294 - 298.
MLA	SEYHAN Sermin,Saribas Gulistan Sanem,COŞKUN AKÇAY NESLİHAN,Gürgen Seren Gülşen,Akyol Seda,GOKTAS GULESER,HIRFANOĞLU İbrahim Murat,ERDOĞAN Deniz,ÖZOĞUL Candan Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity. Gazi Medical Journal, vol.29, no.4, 2018, ss.294 - 298.
AMA	SEYHAN S,Saribas G,COŞKUN AKÇAY N,Gürgen S,Akyol S,GOKTAS G,HIRFANOĞLU İ,ERDOĞAN D,ÖZOĞUL C Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity. Gazi Medical Journal. 2018; 29(4): 294 - 298.
Vancouver	SEYHAN S,Saribas G,COŞKUN AKÇAY N,Gürgen S,Akyol S,GOKTAS G,HIRFANOĞLU İ,ERDOĞAN D,ÖZOĞUL C Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity. Gazi Medical Journal. 2018; 29(4): 294 - 298.
IEEE	SEYHAN S,Saribas G,COŞKUN AKÇAY N,Gürgen S,Akyol S,GOKTAS G,HIRFANOĞLU İ,ERDOĞAN D,ÖZOĞUL C "Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity." Gazi Medical Journal, 29, ss.294 - 298, 2018.
ISNAD	SEYHAN, Sermin vd. "Investigation of the Protective Effects of Acetyl L-Carnitine on Cisplatin-Induced Uterus Toxicity". Gazi Medical Journal 29/4 (2018), 294-298.