Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?

Numan KARAARSLAN, (Namık Kemal University, Faculty of Medicine, Department of Neurosurgery, Tekirdağ, Turkey)
İbrahim YILMAZ, (Istanbul Medipol University, Faculty of Medicine, Department of Medical Pharmacology, Istanbul, Turkey)
DUYGU YAŞAR ŞİRİN, (Namik Kemal University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Tekirdag, Turkey)
Hanefi ÖZBEK, (Istanbul Medipol University, Faculty of Medicine, Department of Medical Pharmacology, Istanbul, Turkey)
Yasin Emre KAYA, (Abant Izzet Baysal University, Faculty of Medicine, Department of Orthopaedic and Traumatology, Bolu, Turkey)
Yener AKYUVA, (Gaziosmanpasa Taksim Research and Training Hospital, Department of Neurosurgery, Istanbul, Turkey)
Necati KAPLAN, (Istanbul Rumeli University, Reyap Hospital, Department of Neurosurgery, Tekirdag, Turkey)
Mustafa DOĞAN, (Namik Kemal University, Faculty of Medicine, Department of Infectious Diseases, Tekirdag, Turkey)
Seyit Ali GÜMÜŞTAŞ, (Dr. Lutfi Kirdar Training and Research Hospital, Department of Orthopaedic and Traumatology, Istanbul, Turkey)
Özkan ATEŞ, (Istanbul Esenyurt University, Esencan Hospital, Department of Neurosurgery, Istanbul, Turkey)
İlknur ERDEM,
Annals of Medical Research
6 3
Yıl: 2018 Cilt: 25 Sayı: 4 Sayfa Aralığı: 756 - 762 Metin Dili: İngilizce DOI: 10.5455/annalsmedres.2018.06.110 İndeks Tarihi: 13-05-2019

Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?

Öz:
Aim: In this study, it was firstly aimed to investigate the effect of Daptomycin (DAP) on the proliferation in Vancomycin (VCM)-administered primary chondrocyte cultures and non-drug-administered primary chondrocyte cultures. Our second objective was to investigate the effects of DAP and VCM on the NP-specific marker protein chondroadherin (CHAD), which is associated with spinal cord and dorsal column growth, on the transcription factor-1 alpha (HIF-1α), which is induced by hypoxia, and on a type II collagen (COL2A1), which is also known to play a significant role in the development of extracellular matrix, at the pharmaco-molecular level.Material and Methods: Standard human primary chondrocyte cultures were established. DAP and VCM were added to the samples. In all groups, molecular analysis was performed at 0th, 24th and 48th hours. In addition, the surface morphology of the cells was evaluated.Results: Changes in cell morphology and cell death in cultures were observed 24 hours after administration of antibiotics to cell cultures. It was observed that drug administration was associated with the cell viability and that cell viability rate for two antibiotics was similar at the 0th and 48th hours. The expression of three genes decreased at the 24th hour in the experimental group where DAP was administered.Conclusion: Thanks to this molecular-based research, it should not be forgotten that DAP and VCM active pharmacological agents, especially used in the treatment of Methicillin-resistant Staphylococcus aureus induced surgical infections, have a negative effect on human chondrocyte and ECM components.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Karaarslan N, Yılmaz İ, YAŞAR ŞİRİN D, özbek h, Kaya Y, akyuva y, KAPLAN N, DOĞAN M, GUMUSTAS S, ATEŞ Ö, ERDEM İ (2018). Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?. , 756 - 762. 10.5455/annalsmedres.2018.06.110
Chicago Karaarslan Numan,Yılmaz İbrahim,YAŞAR ŞİRİN DUYGU,özbek hanefi,Kaya Yasin Emre,akyuva yener,KAPLAN NECATİ,DOĞAN Mustafa,GUMUSTAS Seyit Ali,ATEŞ Özkan,ERDEM İlknur Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?. (2018): 756 - 762. 10.5455/annalsmedres.2018.06.110
MLA Karaarslan Numan,Yılmaz İbrahim,YAŞAR ŞİRİN DUYGU,özbek hanefi,Kaya Yasin Emre,akyuva yener,KAPLAN NECATİ,DOĞAN Mustafa,GUMUSTAS Seyit Ali,ATEŞ Özkan,ERDEM İlknur Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?. , 2018, ss.756 - 762. 10.5455/annalsmedres.2018.06.110
AMA Karaarslan N,Yılmaz İ,YAŞAR ŞİRİN D,özbek h,Kaya Y,akyuva y,KAPLAN N,DOĞAN M,GUMUSTAS S,ATEŞ Ö,ERDEM İ Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?. . 2018; 756 - 762. 10.5455/annalsmedres.2018.06.110
Vancouver Karaarslan N,Yılmaz İ,YAŞAR ŞİRİN D,özbek h,Kaya Y,akyuva y,KAPLAN N,DOĞAN M,GUMUSTAS S,ATEŞ Ö,ERDEM İ Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?. . 2018; 756 - 762. 10.5455/annalsmedres.2018.06.110
IEEE Karaarslan N,Yılmaz İ,YAŞAR ŞİRİN D,özbek h,Kaya Y,akyuva y,KAPLAN N,DOĞAN M,GUMUSTAS S,ATEŞ Ö,ERDEM İ "Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?." , ss.756 - 762, 2018. 10.5455/annalsmedres.2018.06.110
ISNAD Karaarslan, Numan vd. "Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?". (2018), 756-762. https://doi.org/10.5455/annalsmedres.2018.06.110
APA Karaarslan N, Yılmaz İ, YAŞAR ŞİRİN D, özbek h, Kaya Y, akyuva y, KAPLAN N, DOĞAN M, GUMUSTAS S, ATEŞ Ö, ERDEM İ (2018). Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?. Annals of Medical Research, 25(4), 756 - 762. 10.5455/annalsmedres.2018.06.110
Chicago Karaarslan Numan,Yılmaz İbrahim,YAŞAR ŞİRİN DUYGU,özbek hanefi,Kaya Yasin Emre,akyuva yener,KAPLAN NECATİ,DOĞAN Mustafa,GUMUSTAS Seyit Ali,ATEŞ Özkan,ERDEM İlknur Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?. Annals of Medical Research 25, no.4 (2018): 756 - 762. 10.5455/annalsmedres.2018.06.110
MLA Karaarslan Numan,Yılmaz İbrahim,YAŞAR ŞİRİN DUYGU,özbek hanefi,Kaya Yasin Emre,akyuva yener,KAPLAN NECATİ,DOĞAN Mustafa,GUMUSTAS Seyit Ali,ATEŞ Özkan,ERDEM İlknur Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?. Annals of Medical Research, vol.25, no.4, 2018, ss.756 - 762. 10.5455/annalsmedres.2018.06.110
AMA Karaarslan N,Yılmaz İ,YAŞAR ŞİRİN D,özbek h,Kaya Y,akyuva y,KAPLAN N,DOĞAN M,GUMUSTAS S,ATEŞ Ö,ERDEM İ Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?. Annals of Medical Research. 2018; 25(4): 756 - 762. 10.5455/annalsmedres.2018.06.110
Vancouver Karaarslan N,Yılmaz İ,YAŞAR ŞİRİN D,özbek h,Kaya Y,akyuva y,KAPLAN N,DOĞAN M,GUMUSTAS S,ATEŞ Ö,ERDEM İ Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?. Annals of Medical Research. 2018; 25(4): 756 - 762. 10.5455/annalsmedres.2018.06.110
IEEE Karaarslan N,Yılmaz İ,YAŞAR ŞİRİN D,özbek h,Kaya Y,akyuva y,KAPLAN N,DOĞAN M,GUMUSTAS S,ATEŞ Ö,ERDEM İ "Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?." Annals of Medical Research, 25, ss.756 - 762, 2018. 10.5455/annalsmedres.2018.06.110
ISNAD Karaarslan, Numan vd. "Does transcription factor, induced by daptomycin and vancomycin, affect HIF-1α, Chondroadherin, and COL2A1?". Annals of Medical Research 25/4 (2018), 756-762. https://doi.org/10.5455/annalsmedres.2018.06.110
Sayfa Oluşturulma Tarihi
23-04-2024 7:46

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