The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143

BAYRAKTAR, MELTEM; Gurel, Aynur; ÖZBEK, Nedim; HAYTA SMEDLEY, Şadiye; ANSIZ, Arif

The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143

Şadiye HAYTA SMEDLEY, (Ege University, Faculty of Engineering, Bioengineering Department, Izmir, Turkey)

Nedim ÖZBEK, (Nazilli Cotton Research Institute, Aydin, Turkey)

Arif ANSIZ, (Ege University, Faculty of Engineering, Bioengineering Department, Izmir, Turkey)

Meltem BAYRAKTAR, (Ahi Evran University, Faculty of Engineering and Architecture, Genetic and Bioengineering Department, Kirsehir, Turkey)

AYNUR GÜREL (Ege University, Faculty of Engineering, Bioengineering Department, Izmir, Turkey)

ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi

0 0

Yıl: 2018 Cilt: 28 Sayı: 2 Sayfa Aralığı: 55 - 61 Metin Dili: İngilizce İndeks Tarihi: 14-05-2019

The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143

Öz:

A simple and reliable protocol has been developed for plantlet regeneration through somaticembryogenesis from suspension cultures of Gossypium hirsutum L. cv. Nazilli-143. Embryogenic callus was initiated fromhypocotyl tissues of 7-day-old seedlings. High induction frequencies of the embryogenic callus were obtained on mediumcontaining Murashige and Skoog (MS) salts, Gamborg’s B5 medium (B5) vitamins, 30 g L-1 glucose, 0.75 g L-1 MgCl2, 0.1 mg L-1Kinetin and 0.1 mg L-1 2,4-Dichlorophenoxyacetic acid (2,4-D) and the medium was solidified using 0.7% (w/v) agar (pH 5.8).Embryogenic calli were placed on plant growth regulator (PGR)-free liquid MS medium in order to establish suspension culturesfor somatic embryo induction. Suspensions were sieved and the globular somatic embryos were collected and plated onto varioustypes of semi-solid media. Embryo proliferation and maturation processes were best observed on medium containing 2/3 MS plus1.3 g L-1 KNO3 free from PGR. Plantlets were recovered from 36 % of the matured embryos. Plantlets with a root system and trueleaves were removed from the sterile culture and were transferred into a plant growth chamber.

Anahtar Kelime:

Nazilli-143 Pamuk (Gossypium hirsutum L.) Genotipinde Farklı Bitki Büyüme Düzenleyicilerinin Hipokotil Eksplantlarından Kallus İndüksiyonu Üzerine Etkisi ve Somatik Embriyo Aracılığıyla Bitkicik Rejenerasyonu

Öz:

Gossypium hirsutum L. cv. Nazilli-143’ün hücre süspansiyon kültürlerinden somatik embriyo yolu ile bitkicik rejenerasyonu için basit ve güvenilir bir protokol geliştirilmiştir. Embriyogenik kallus 7 günlük fideciklerin hipokotil dokularından başlatılmıştır. En yüksek embriyogenik kallus oranı Murashige ve Skoog (MS) besin ortamının makro ve mikro elementleri, Gamborg (B5) besin ortamının vitaminleri ile 30 g L-1 glukoz, 0,75 g L-1 MgCl2, 0,1 mg L-1 Kinetin ve 0,1 mg L-1 2,4 Diklorofenoksi asetik asit (2,4-D) içeren ve % 0,7 (w/v) agar ile yarı katı hale getirilmiş besin ortamında (pH 5.8) elde edilmiştir. Somatik embriyo indüksiyonu için süspansiyon kültürlerini kurmak amacıyla, embriyogenik kalluslar bitki büyüme düzenleyicisi içermeyen sıvı MS besin ortamında kültüre alınmıştır. Süspansiyon kültürleri süzülmüş ve globular yapıdaki somatik embriyolar toplanarak çeşitli yarı katı besin ortamlarına aktarılmışlardır. Embriyo çoğaltımı ve olgunlaşması en iyi, 1,3 g L-1 KNO3 eklenmiş, bitki büyüme düzenleyicisi içermeyen 2/3 MS besin ortamında gözlenmiştir. Olgunlaşmış embriyoların % 36’sından bitkiciğe dönüşüm gerçekleşmiştir. Köklü ve gerçek yapraklara sahip bitkicikler steril kültürden uzaklaştırılmış ve bitki büyüme kabinine transfer edilmişlerdir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Aydin, Y., Z. Ipekci, T. Talas-Ogras, A. Zehir, A, K. Bayrovic, and N. Gozukirmizi. 2004 High frequency somatic embryogenesis in cotton. Biol. Plantarum 48 (4): 491-495.
Davidonis, G. H., and R. H. Hamilton. 1983. Plant regeneration from callus tissue of Gossypium hirsutum L. Plant Science Letters 32: 89-93.
Finer, J. J. 1988. Plant regeneration from somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.). Plant Cell Rep. 7: 481-494.
Firoozabady, E., and D. L. Deboer. 1993. Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.). In Vitro Cell. Dev. Plant 299: 166-173.
Gamborg, O. L., R. A. Miller, and K. Ojima. 1968. Nutrition requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-159.
Ganesan, M., R. Chandrasekar, B. D. Ranjitha Kumari, and N. Jayabalan. 2007. Somatic embryogenesis and plant regeneration of Abelmoschus esculentus through suspension culture. Biol. Plantarum 51 (3): 414-420.
Juturu, V. N., G. K. Mekala, and P. B. Kirti 2015. Current status of tissue culture and genetic transformation research in cotton (Gossypium spp.). Plant Cell Tissue and Organ Cult. 120: 813-839.
Khan, T., V. S. Reddy, and S. Leelavathi. 2010. Highfrequency regeneration via somatic embryogenesis of an elite recalcitrant cotton genotype (Gossypium hirsutum L.) and efficient Agrobacterium-mediated transformation. Plant Cell Tissue Organ Cult. 101: 323-330.
Kumria, R., V. G. Sunnichan, D. K. Das, S. K. Gupta, V. S. Reddy, R. K. Bhatnagar, and S. Leelavathi. 2003a. High-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum) through metabolic stress. Plant Cell Rep. 21: 635-639.
Kumria, R., S. Leelavathi, R. K. Bhatnagar, and V. S. Reddy. 2003b. Regeneration and genetic transformation of cotton: Present status and future perspectives. Plant Tissue Culture 13: 211-225.
Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15: 473-497.
Price, H. J., and R. H. Smith. 1979. Somatic embryogenesis in suspension cultures of Gossypium klotzschianum Anderss. Planta 145: 305-307.
Shoemaker, R.C., L. J. Couche, and D. W. Galbraith. 1986. Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.). Plant Cell Rep. 3: 178-181.
Trolinder, N. L. and J. R. Goodin. 1988a. Somatic embryogenesis in cotton (Gossypium) I. Effects of source of explant and hormone regime. Plant Cell Tissue Organ Cult. 12: 178-181.
Trolinder, N. L., and J. R. Goodin. 1988b. Somatic embryogenesis in cotton (Gossypium) II. Requirements for embryo development and plant regeneration. Plant Cell Tissue Organ Cult. 12: 43-53.
Wang, Y. X., X. F. Wang, Z. Y. Ma, G. Y. Zhang, and G. Y. Han. 2006. Somatic embryogenesis and plant regeneration from two recalcitrant genotypes of Gossypium hirsutum L. Agricultural Sciences in China 5 (5): 323-329.
Zhang, B. H., F. Liu, and C. B. Yak. 2000. Plant regeneration via somatic embryogenesis in cotton. Plant Cell Tissue Organ Cult. 60: 89-94.

APA	HAYTA SMEDLEY Ş, ÖZBEK N, ANSIZ A, BAYRAKTAR M, Gurel A (2018). The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. , 55 - 61.
Chicago	HAYTA SMEDLEY Şadiye,ÖZBEK Nedim,ANSIZ Arif,BAYRAKTAR MELTEM,Gurel Aynur The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. (2018): 55 - 61.
MLA	HAYTA SMEDLEY Şadiye,ÖZBEK Nedim,ANSIZ Arif,BAYRAKTAR MELTEM,Gurel Aynur The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. , 2018, ss.55 - 61.
AMA	HAYTA SMEDLEY Ş,ÖZBEK N,ANSIZ A,BAYRAKTAR M,Gurel A The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. . 2018; 55 - 61.
Vancouver	HAYTA SMEDLEY Ş,ÖZBEK N,ANSIZ A,BAYRAKTAR M,Gurel A The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. . 2018; 55 - 61.
IEEE	HAYTA SMEDLEY Ş,ÖZBEK N,ANSIZ A,BAYRAKTAR M,Gurel A "The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143." , ss.55 - 61, 2018.
ISNAD	HAYTA SMEDLEY, Şadiye vd. "The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143". (2018), 55-61.

APA	HAYTA SMEDLEY Ş, ÖZBEK N, ANSIZ A, BAYRAKTAR M, Gurel A (2018). The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, 28(2), 55 - 61.
Chicago	HAYTA SMEDLEY Şadiye,ÖZBEK Nedim,ANSIZ Arif,BAYRAKTAR MELTEM,Gurel Aynur The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi 28, no.2 (2018): 55 - 61.
MLA	HAYTA SMEDLEY Şadiye,ÖZBEK Nedim,ANSIZ Arif,BAYRAKTAR MELTEM,Gurel Aynur The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, vol.28, no.2, 2018, ss.55 - 61.
AMA	HAYTA SMEDLEY Ş,ÖZBEK N,ANSIZ A,BAYRAKTAR M,Gurel A The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi. 2018; 28(2): 55 - 61.
Vancouver	HAYTA SMEDLEY Ş,ÖZBEK N,ANSIZ A,BAYRAKTAR M,Gurel A The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143. ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi. 2018; 28(2): 55 - 61.
IEEE	HAYTA SMEDLEY Ş,ÖZBEK N,ANSIZ A,BAYRAKTAR M,Gurel A "The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143." ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi, 28, ss.55 - 61, 2018.
ISNAD	HAYTA SMEDLEY, Şadiye vd. "The Effect of Different Plant Growth Regulators on Callus Induction from Hypocotyl Explants and Plantlet Regeneration Through Somatic Embryo in Cotton (Gossypium hirsutum L.) Genotype Nazilli-143". ANADOLU Ege Tarımsal Araştırma Enstitüsü Dergisi 28/2 (2018), 55-61.