Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary
Yıl: 2018 Cilt: 50 Sayı: 3 Sayfa Aralığı: 160 - 163 Metin Dili: İngilizce DOI: 10.5152/eurasianjmed.2018.17361 İndeks Tarihi: 12-06-2019
Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary
Öz: Objective: To analyze the effects of melatonin on vascular endothelial growth factor A (VEGF-A) expression and follicle reserve in rat ovary.Materials and Methods: A total of 45 female Wistar rats were used in the present study. Rats were divided into three groups: group 1 (control), group 2 (vehicle), and group 3 (melatonin). Rats in the melatonin group were treated with an intraperitoneal injection of melatonin at a dose of 50 mg/kg/day for 56 days. We investigated VEGF-A expression in rat ovary in all the groups using Western blot and reverse transcription- polymerase chain reaction. Histopathological parameters were evaluated using light microscopy.Results: The number of atretic follicles was significantly lower in the melatonin treatment rats than in the control rats (p<0.05); however, the number of antral follicles was significantly higher in the former (p<0.05). Additionally, we observed a weak immunoblot stain in the melatonin group for VEGF-A protein. Interest- ingly, melatonin treatment induced a significant decrease in VEGF-A expression in the ovary of group 3 rats (p<0.05), whereas no such difference was observed between group 1 and group 2 rats (p>0.05).Conclusion: The present study demonstrates that the protective effect of melatonin on the degeneration of follicles in rat ovary is reduced by decreasing the VEGF-A expression. These results suggest that melatonin is effective against follicular atresia and preserves antral follicles, thus, offering a therapeutic advantage in clinical use.
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- Behram Kandemir Y, Aydin C, Gorgisen G. The effects of melatonin on oxidative stress and pre- vention of primordial follicle loss via activation of mTOR pathway in the rat ovary. Cell Mol Biol (Noisy-le-grand) 2017; 63: 100-6. [CrossRef]
- Inkster CF, Ng SG, Leatherbarrow B. Primary banked scleral patch graft in the prevention of exposure of hydroxyapatite orbital implants. Ophthalmology 2002; 109: 389-92. [CrossRef]
- Ma J, Liu T, Qu J. The effect of orbital implantation on peripheral blood melatonin and sex hormone
- levels in child patients with congenital eyeball dys- plasia. Exp Ther Med 2017;14:2211-5. [CrossRef]
- Bates DO, Harper SJ. Regulation of vascular per- meability by vascular endothelial growth factors. Vascul Pharmacol 2002; 39: 225-37. [CrossRef]
- Chen S, Qin C, Sin JE, et al. Discovery of novel dual VEGFR2 and Src inhibitors using a multistep virtual screening approach. Future Med Chem 2017; 9: 7-24. [CrossRef]
- Shibuya M. Vascular Endothelial Growth Factor (VEGF) and Its Receptor (VEGFR) Signaling in Angiogenesis: A Crucial Target for Anti-and Pro- Angiogenic Therapies. Genes Cancer 2011; 2: 1097-105. [CrossRef]
- Tischer E, Mitchell R, Hartman T, et al. The hu- man gene for vascular endothelial growth factor. Multiple protein forms are encoded through al- ternative exon splicing. J Biol Chem 1991; 266: 11947-54.
- Cross MJ, Dixelius J, Matsumoto T, Claesson- Welsh L. VEGF-receptor signal transduction. Trends Biochem Sci 2003; 28: 488-94. [CrossRef]
- Lissoni P, Rovelli F, Malugani F, Bucovec R, Conti A, Maestroni GJ. Anti-angiogenic activity of mela- tonin in advanced cancer patients. Neuro Endo- crinol Lett 2001; 22: 45-7.
- Tilly JL. Ovarian follicle counts--not as simple as 1, 2, 3. Reprod Biol Endocrinol 2003; 1: 11. [CrossRef]
- Mehlmann LM, Saeki Y, Tanaka S, Brennan TJ, Evsikov AV, Pendola FL, et al. The Gs-linked re- ceptor GPR3 maintains meiotic arrest in mam- malian oocytes. Science 2004; 306: 1947-50. [CrossRef]
- Loren AW, Mangu PB, Beck LN, et al. Fertility preservation for patients with cancer: Ameri- can Society of Clinical Oncology clinical practice guideline update. J Clin Oncol 2013; 31: 2500- [CrossRef]
- Goswami D, Conway GS. Premature ovarian fail- ure. Horm Res 2007; 68: 196-202. [CrossRef]
- Reddy P, Liu L, Adhikari D, et al. Oocyte-specific deletion of Pten causes premature activation of the primordial follicle pool. Science 2008; 319: 611-3. [CrossRef]
- Hong X, Luense LJ, McGinnis LK, Nothnick WB, Christenson LK. Dicer1 is essential for female fertility and normal development of the female reproductive system. Endocrinology 2008; 149: 6207-12. [CrossRef]
- Garcia-Navarro A, Gonzalez-Puga C, Escames G, et al. Cellular mechanisms involved in the mela- tonin inhibition of HT-29 human colon cancer cell proliferation in culture. J Pineal Res 2007; 43: 195-205. [CrossRef]
- Maldonado MD, Siu AW, Sanchez-Hidalgo M, Acuna-Castroviejo D, Escames G. Melatonin and lipid uptake by murine fibroblasts: clinical implica- tions. Neuro Endocrinol Lett 2006; 27: 601-8.
- Reiter RJ, Mayo JC, Tan DX, Sainz RM, Alatorre- Jimenez M, Qin L. Melatonin as an antioxidant: under promises but over delivers. J Pineal Res 2016; 61: 253-78. [CrossRef]
- Horton TH, Yellon SM. Aging, reproduction, and the melatonin rhythm in the Siberian hamster. J Biol Rhythms 2001; 16: 243-53. [CrossRef]
- Ma M, Chen XY, Li B, Li XT. Melatonin pro- tects premature ovarian insufficiency induced by tripterygium glycosides: role of SIRT1. Am J Transl Res 2017; 9: 1580-602.
- Chen XY, Gu C, Ma M, et al. A mouse model of premature ovarian insufficiency induced by tripterygium glycoside via subcutaneous injec- tion. Int J Clin Exp Pathol 2014; 7: 144-51.
- Chen XY, Xia HX, Guan HY, Li B, Zhang W. Fol- licle Loss and Apoptosis in Cyclophosphamide- Treated Mice: What’s the Matter? Int J Mol Sci 2016 May 30;17: E836. [CrossRef]
- Chan KA, Tsoulis MW, Sloboda DM. Early-life nu- tritional effects on the female reproductive sys- tem. J Endocrinol 2015; 224: R45-62. [CrossRef]
- Bergers G, Benjamin LE. Tumorigenesis and the angiogenic switch. Nat Rev Cancer 2003; 3: 401- [CrossRef]
- Jardim-Perassi BV, Arbab AS, Ferreira LC, et al. Effect of melatonin on tumor growth and an- giogenesis in xenograft model of breast cancer. PLoS One 2014; 9: e85311. [CrossRef]
- Romeu LR, da Motta EL, Maganhin CC, et al. Ef- fects of melatonin on histomorphology and on the expression of steroid receptors, VEGF, and PCNA in ovaries of pinealectomized female rats. Fertil Steril 2011; 95:1379-84. [CrossRef]
- Greenaway J, Connor K, Pedersen HG, Coomber BL, LaMarre J, Petrik J. Vascular endo- thelial growth factor and its receptor, Flk-1/KDR, are cytoprotective in the extravascular compart- ment of the ovarian follicle. Endocrinology 2004; 145: 2896-905. [CrossRef]
| APA | Behram Y, KONUK E, BEHRAM M, sindel m (2018). Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary. , 160 - 163. 10.5152/eurasianjmed.2018.17361 |
| Chicago | Behram Yasemin,KONUK Esma,BEHRAM MUSTAFA,sindel muzaffer Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary. (2018): 160 - 163. 10.5152/eurasianjmed.2018.17361 |
| MLA | Behram Yasemin,KONUK Esma,BEHRAM MUSTAFA,sindel muzaffer Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary. , 2018, ss.160 - 163. 10.5152/eurasianjmed.2018.17361 |
| AMA | Behram Y,KONUK E,BEHRAM M,sindel m Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary. . 2018; 160 - 163. 10.5152/eurasianjmed.2018.17361 |
| Vancouver | Behram Y,KONUK E,BEHRAM M,sindel m Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary. . 2018; 160 - 163. 10.5152/eurasianjmed.2018.17361 |
| IEEE | Behram Y,KONUK E,BEHRAM M,sindel m "Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary." , ss.160 - 163, 2018. 10.5152/eurasianjmed.2018.17361 |
| ISNAD | Behram, Yasemin vd. "Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary". (2018), 160-163. https://doi.org/10.5152/eurasianjmed.2018.17361 |
| APA | Behram Y, KONUK E, BEHRAM M, sindel m (2018). Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary. Eurasian Journal of Medicine, 50(3), 160 - 163. 10.5152/eurasianjmed.2018.17361 |
| Chicago | Behram Yasemin,KONUK Esma,BEHRAM MUSTAFA,sindel muzaffer Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary. Eurasian Journal of Medicine 50, no.3 (2018): 160 - 163. 10.5152/eurasianjmed.2018.17361 |
| MLA | Behram Yasemin,KONUK Esma,BEHRAM MUSTAFA,sindel muzaffer Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary. Eurasian Journal of Medicine, vol.50, no.3, 2018, ss.160 - 163. 10.5152/eurasianjmed.2018.17361 |
| AMA | Behram Y,KONUK E,BEHRAM M,sindel m Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary. Eurasian Journal of Medicine. 2018; 50(3): 160 - 163. 10.5152/eurasianjmed.2018.17361 |
| Vancouver | Behram Y,KONUK E,BEHRAM M,sindel m Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary. Eurasian Journal of Medicine. 2018; 50(3): 160 - 163. 10.5152/eurasianjmed.2018.17361 |
| IEEE | Behram Y,KONUK E,BEHRAM M,sindel m "Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary." Eurasian Journal of Medicine, 50, ss.160 - 163, 2018. 10.5152/eurasianjmed.2018.17361 |
| ISNAD | Behram, Yasemin vd. "Effect of Melatonin on the Expression of VEGF-A and on the Degeneration of Follicle Reserve in Rat Ovary". Eurasian Journal of Medicine 50/3 (2018), 160-163. https://doi.org/10.5152/eurasianjmed.2018.17361 |
