Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats
Yıl: 2018 Cilt: 48 Sayı: 1 Sayfa Aralığı: 196 - 201 Metin Dili: İngilizce DOI: 10.3906/sag-1709-160 İndeks Tarihi: 19-06-2019
Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats
Öz: Background/aim: This study aimed to investigate signal peptide-Cub-EGF domain-containing protein 1 (SCUBE1) levels and oxidativestress (OS) in a high fat diet (HFD)-induced obese rat model.Materials and methods: Fourteen rats were randomly divided into two groups. Group 1, the control group, was given a standard diet(10% calories of fat) and Group 2, the obese group, was given a HFD (60% calories of fat), both for 70 days. Rats were then sacrificed andserum samples were collected. Serum glucose and triglyceride concentrations were determined using an autoanalyzer. Serum SCUBE1,adiponectin, and plasminogen activator inhibitor-1 levels were determined using an enzyme-linked immunosorbent assay. Serummalondialdehyde levels and superoxide dismutase and glutathione peroxidase enzyme activities were determined using colorimetricmethods.Results: Final body weight was higher in the obese group (P = 0.007). Serum malondialdehyde concentrations were also higher in theobese group (P = 0.021). Serum glutathione peroxidase activities were higher in the control group (P = 0.028). Serum SCUBE1 levelswere also higher in the control group (P = 0.038).Conclusion: There may be no connection between the measured OS parameters and SCUBE1. Differences in SCUBE1 levels maytherefore be evaluated independently from OS in obesity
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Via MA, Mechanick JI. Obesity as a disease. Curr Obes Rep 2014; 3: 291-297.
- Galic S, Oakhill JS, Steinberg GR. Adipose tissue as an endocrine organ. Mol Cell Endocrinol 2010; 316: 129-139.
- Vazquez-Vela ME, Torres N, Tovar AR. White adipose tissue as endocrine organ and its role in obesity. Arch Med Res 2008; 39: 715-728.
- Ruskovska T, Bernlohr DA. Oxidative stress and protein carbonylation in adipose tissue - implications for insulin resistance and diabetes mellitus. J Proteomics 2013; 92: 323- 334.
- Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39: 44-84.
- Vincent HK, Taylor AG. Biomarkers and potential mechanisms of obesity-induced oxidant stress in humans. Int J Obes (Lond) 2006; 30: 400-418.
- Wu BT, Su YH, Tsai MT, Wasserman SM, Topper JN, Yang RB. A novel secreted, cell-surface glycoprotein containing multiple epidermal growth factor-like repeats and one CUB domain is highly expressed in primary osteoblasts and bones. J Biol Chem 2004; 279: 37485-37490.
- Yang RB, Ng CK, Wasserman SM, Colman SD, Shenoy S, Mehraban F, Komuves LG, Tomlinson JE, Topper JN. Identification of a novel family of cell-surface proteins expressed in human vascular endothelium. J Biol Chem 2002; 277: 46364-46373.
- Dai DF, Thajeb P, Tu CF, Chiang FT, Chen CH, Yang RB, Chen JJ. Plasma concentration of SCUBE1, a novel platelet protein, is elevated in patients with acute coronary syndrome and ischemic stroke. J Am Coll Cardiol 2008; 51: 2173-2180.
- Mentese A, Fidan E, Sumer AU, Karahan SC, Sonmez M, Altay DU, Kavgaci H, Alver A. Is SCUBE 1 a new biomarker for gastric cancer? Cancer Biomark 2012; 11: 191-195.
- Turkmen S, Eryigit U, Karaca Y, Mentese A, Sumer UA, Yulug E, Aksut N, Gazioglu S, Gunduz A. Diagnostic value of plasma signal peptide-Cub-Egf domain-containing protein-1 (SCUBE-1) in an experimental model of acute ischemic stroke. Am J Emerg Med 2015; 33: 262-265.
- Turkmen S, Mentese S, Mentese A, Sumer AU, Saglam K, Yulug E, Turedi S, Gunduz A. The value of signal peptide-CUB-EGF domain-containing protein 1 and oxidative stress parameters in the diagnosis of acute mesenteric ischemia. Acad Emerg Med 2013; 20: 257-264.
- Tu CF, Su YH, Huang YN, Tsai MT, Li LT, Chen YL, Cheng CJ, Dai DF, Yang RB. Localization and characterization of a novel secreted protein SCUBE1 in human platelets. Cardiovasc Res 2006; 71: 486-495.
- Maury E, Brichard SM. Adipokine dysregulation, adipose tissue inflammation and metabolic syndrome. Mol Cell Endocrinol 2010; 314: 1-16.
- Samad F, Ruf W. Inflammation, obesity, and thrombosis. Blood 2013; 122: 3415-3422.
- Yagi K. Lipid peroxides and related radicals in clinical medicine. Adv Exp Med Biol 1994; 366: 1-15.
- Buettner R, Scholmerich J, Bollheimer LC. High-fat diets: modeling the metabolic disorders of human obesity in rodents. Obesity (Silver Spring) 2007; 15: 798-808.
- Buettner R, Parhofer KG, Woenckhaus M, Wrede CE, KunzSchughart LA, Scholmerich J, Bollheimer LC. Defining highfat-diet rat models: metabolic and molecular effects of different fat types. J Mol Endocrinol 2006; 36: 485-501.
- Bake T, Morgan DG, Mercer JG. Feeding and metabolic consequences of scheduled consumption of large, binge-type meals of high fat diet in the Sprague-Dawley rat. Physiol Behav 2014; 128: 70-79.
- Öner-İyidoğan Y, Koçak H, Seyidhanoğlu M, Gürdöl F, Gülçubuk A, Yildirim F, Çevik A, Uysal M. Curcumin prevents liver fat accumulation and serum fetuin-A increase in rats fed a high-fat diet. J Physiol Biochem 2013; 69: 677-686.
- Gallou-Kabani C, Vigé A, Gross MS, Rabès JP, Boileau C, Larue-Achagiotis C, Tomé D, Jais JP, Junien C. C57BL/6J and A/J mice fed a high-fat diet delineate components of metabolic syndrome. Obesity (Silver Spring) 2007; 15: 1996-2005.
- Kusminski CM, Scherer PE. Mitochondrial dysfunction in white adipose tissue. Trends Endocrinol Metab 2012; 23: 435- 443.
- Trayhurn P, Wang B, Wood IS. Hypoxia in adipose tissue: a basis for the dysregulation of tissue function in obesity? Br J Nutr 2008; 100: 227-235.
- Fernández-Sánchez A, Madrigal-Santillán E, Bautista M, Esquivel-Soto J, Morales-González Á, Esquivel-Chirino C, Durante-Montiel I, Sánchez-Rivera G, Valadez-Vega C, Morales-González JA. Inflammation, oxidative stress, and obesity. Int J Mol Sci 2011; 12: 3117-3132.
- Le Lay S, Simard G, Martinez MC, Andriantsitohaina R. Oxidative stress and metabolic pathologies: from an adipocentric point of view. Oxid Med Cell Longev 2014; 2014: 908539.
- Emami SR, Jafari M, Haghshenas R, Ravasi A. Impact of eight weeks endurance training on biochemical parameters and obesity-induced oxidative stress in high fat diet-fed rats. J Exerc Nutrition Biochem 2016; 20: 29-35.
- Carmiel-Haggai M, Cederbaum AI, Nieto N. A high-fat diet leads to the progression of non-alcoholic fatty liver disease in obese rats. FASEB J 2005; 19: 136-138.
- Haluzik M, Gavrilova O, LeRoith D. Peroxisome proliferatoractivated receptor-alpha deficiency does not alter insulin sensitivity in mice maintained on regular or high-fat diet: hyperinsulinemic-euglycemic clamp studies. Endocrinology 2004; 145: 1662-1667.
- Ye JM, Iglesias MA, Watson DG, Ellis B, Wood L, Jensen PB, Sørensen RV, Larsen PJ, Cooney GJ, Wassermann K et al. PPARα/γ ragaglitazar eliminates fatty liver and enhances insulin action in fat-fed rats in the absence of hepatomegaly. Am J Physiol Endocrinol Metab 2003; 284: E531-540.
- Tu CF, Yan YT, Wu SY, Djoko B, Tsai MT, Cheng CJ, Yang RB. Domain and functional analysis of a novel platelet-endothelial cell surface protein, SCUBE1. J Biol Chem 2008; 283: 12478- 12488.
- Yoo HJ, Choi KM. Adipokines as a novel link between obesity and atherosclerosis. World J Diabetes 2014; 5: 357-363.
- Eckel RH, Grundy SM, Zimmet PZ. The metabolic syndrome. Lancet 2005; 365: 1415-1428.
- Liao WJ, Tsao KC, Yang RB. Electrostatics and N-glycanmediated membrane tethering of SCUBE1 is critical for promoting bone morphogenetic protein signalling. Biochem J 2016; 473: 661-672.
- Wu MY, Lin YC, Liao WJ, Tu CF, Chen MH, Roffler SR, Yang RB. Inhibition of the plasma SCUBE1, a novel platelet adhesive protein, protects mice against thrombosis. Arterioscler Thromb Vasc Biol 2014; 34: 1390-1398.
- Ding YS, Sun B, Jiang JX, Zhang Q, Lu J, Gao GZ. Increased serum concentrations of signal peptide-Cub-Egf domaincontaining protein-1 in patients with aneurysmal subarachnoid hemorrhage. Clin Chim Acta 2016; 459: 117-122.
- Morange PE, Alessi MC. Thrombosis in central obesity and metabolic syndrome: mechanisms and epidemiology. Thromb Haemost 2013; 110: 669-680.
| APA | Bodur A, kahraman c, us altay d, RENDI T, Mentese A, Alver A (2018). Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats. , 196 - 201. 10.3906/sag-1709-160 |
| Chicago | Bodur Akın,kahraman cemil,us altay diler,RENDI Taghi Ahmadi,Mentese Ahmet,Alver Ahmet Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats. (2018): 196 - 201. 10.3906/sag-1709-160 |
| MLA | Bodur Akın,kahraman cemil,us altay diler,RENDI Taghi Ahmadi,Mentese Ahmet,Alver Ahmet Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats. , 2018, ss.196 - 201. 10.3906/sag-1709-160 |
| AMA | Bodur A,kahraman c,us altay d,RENDI T,Mentese A,Alver A Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats. . 2018; 196 - 201. 10.3906/sag-1709-160 |
| Vancouver | Bodur A,kahraman c,us altay d,RENDI T,Mentese A,Alver A Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats. . 2018; 196 - 201. 10.3906/sag-1709-160 |
| IEEE | Bodur A,kahraman c,us altay d,RENDI T,Mentese A,Alver A "Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats." , ss.196 - 201, 2018. 10.3906/sag-1709-160 |
| ISNAD | Bodur, Akın vd. "Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats". (2018), 196-201. https://doi.org/10.3906/sag-1709-160 |
| APA | Bodur A, kahraman c, us altay d, RENDI T, Mentese A, Alver A (2018). Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats. Turkish Journal of Medical Sciences, 48(1), 196 - 201. 10.3906/sag-1709-160 |
| Chicago | Bodur Akın,kahraman cemil,us altay diler,RENDI Taghi Ahmadi,Mentese Ahmet,Alver Ahmet Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats. Turkish Journal of Medical Sciences 48, no.1 (2018): 196 - 201. 10.3906/sag-1709-160 |
| MLA | Bodur Akın,kahraman cemil,us altay diler,RENDI Taghi Ahmadi,Mentese Ahmet,Alver Ahmet Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats. Turkish Journal of Medical Sciences, vol.48, no.1, 2018, ss.196 - 201. 10.3906/sag-1709-160 |
| AMA | Bodur A,kahraman c,us altay d,RENDI T,Mentese A,Alver A Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats. Turkish Journal of Medical Sciences. 2018; 48(1): 196 - 201. 10.3906/sag-1709-160 |
| Vancouver | Bodur A,kahraman c,us altay d,RENDI T,Mentese A,Alver A Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats. Turkish Journal of Medical Sciences. 2018; 48(1): 196 - 201. 10.3906/sag-1709-160 |
| IEEE | Bodur A,kahraman c,us altay d,RENDI T,Mentese A,Alver A "Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats." Turkish Journal of Medical Sciences, 48, ss.196 - 201, 2018. 10.3906/sag-1709-160 |
| ISNAD | Bodur, Akın vd. "Investigation of the relationship between oxidative stress and SCUBE1 levels in high fat diet-induced obese rats". Turkish Journal of Medical Sciences 48/1 (2018), 196-201. https://doi.org/10.3906/sag-1709-160 |
