Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions
Yıl: 2018 Cilt: 8 Sayı: 3 Sayfa Aralığı: 211 - 217 Metin Dili: İngilizce DOI: 10.21597/jist.458632 İndeks Tarihi: 17-01-2019
Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions
Öz: Whereas a very small amount of pesticides which are used for elimination of undesirable speciesand for a more fertile agriculture reach the target organism, majority of pesticides reach to nontarget organisms. It isforeseen by our team that the pesticides used for various purposes may negatively affect the glutathione mechanismof the organisms. However, determination of effective dosage range is the objective of our study subjects. For thisreason, the in vitro effects of widely used pesticides (lambda-cyhalothrin, cypermethrin, chlorpyrifos, dichlorvos,glyphosate isopropylamine) on the activities of glutathione reductase (GR) and glutathione S-transferase(GST) which are two important enzymes of the glutathione system have been investigated in this study. It hasbeen determined that when lambda-cyhalothrin does not affect the GR enzyme purified from the turkey liver,chlorpyrifos, glyphosate isopropylamine, dichlorvos and cypermethrin cause inhibition. When the effects ofpesticides on GST enzyme purified from turkey liver were investigated in in vitro conditions, it was determinedthat all examined pesticides had inhibitory effects. In this context, the potential doses which can create a risk forlive life, of pesticides that are commonly used, have been identified.
Anahtar Kelime: Konular:
Hindi Karaciğerinden Saflaştırılan Glutatyon Redüktaz ve Glutatyon S-Transferaz Aktiviteleri Üzerine Bazı Pestisitlerin Etkilerinin in Vitro Şartlarda İncelenmesi
Öz: İstenmeyen türlerin ortadan kaldırılması ve daha verimli bir tarım için kulanılan pestisitlerin, çok küçük bir kısmı hedef organizmaya ulaşırken oldukça büyük bir kısmı da hedef olmayan organizmalara ulaşmaktadır. Çeşitli amaçlarla kullanılan pestisitlerin söz konusu organizmaların glutatyon mekanizmasını olumsuz şekilde etkileyebileceği ekibimiz tarafından öngörülmüştür. Ancak etkili dozaj aralığının tespit edilmesi çalışma konumuzun amacını oluşturmaktadır. Bu nedenle belirtilen çalışmada glutatyon sisteminin iki önemli enzimi olan glutatyon redüktaz (GR) ve glutatyon S-transferazın (GST) aktiviteleri üzerine oldukça yaygın kullanılan pestisitlerin (lambda-sihalotrin, sipermetrin, klorpirifos, diklorvos, glifosat izopropilamin) in vitro etkileri araştırılmıştır. Hindi karaciğerinden saflaştırılan GR enzim aktivitesi üzerine lambda-sihalotrin’nin etki etmediği, klorpirifos, glifosat izopropilaminin, diklorvos ve sipermetrinin ise inhibisyona sebep olduğu tespit edilmiştir. Hindi karaciğerinden saflaştırılan GST enzim aktivitesi üzerine söz konusu pestisitlerin etkileri in vitro şartlarda araştırıldığında ise çalışma kapsamında incelenen tüm pestisitlerin inhibisyon etkisine sahip olduğu belirlenmiştir. Bu kapsamda yaygın olarak kullanılan zirai ilaçların canlı yaşam için risk oluşturabilecek potansiyel dozajları tespit edilmiştir.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | TAŞER GÜLLER P, Akkemik E, kör s, Ciftci M (2018). Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions. , 211 - 217. 10.21597/jist.458632 |
| Chicago | TAŞER GÜLLER PINAR,Akkemik Ebru,kör sevil,Ciftci Mehmet Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions. (2018): 211 - 217. 10.21597/jist.458632 |
| MLA | TAŞER GÜLLER PINAR,Akkemik Ebru,kör sevil,Ciftci Mehmet Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions. , 2018, ss.211 - 217. 10.21597/jist.458632 |
| AMA | TAŞER GÜLLER P,Akkemik E,kör s,Ciftci M Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions. . 2018; 211 - 217. 10.21597/jist.458632 |
| Vancouver | TAŞER GÜLLER P,Akkemik E,kör s,Ciftci M Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions. . 2018; 211 - 217. 10.21597/jist.458632 |
| IEEE | TAŞER GÜLLER P,Akkemik E,kör s,Ciftci M "Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions." , ss.211 - 217, 2018. 10.21597/jist.458632 |
| ISNAD | TAŞER GÜLLER, PINAR vd. "Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions". (2018), 211-217. https://doi.org/10.21597/jist.458632 |
| APA | TAŞER GÜLLER P, Akkemik E, kör s, Ciftci M (2018). Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8(3), 211 - 217. 10.21597/jist.458632 |
| Chicago | TAŞER GÜLLER PINAR,Akkemik Ebru,kör sevil,Ciftci Mehmet Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi 8, no.3 (2018): 211 - 217. 10.21597/jist.458632 |
| MLA | TAŞER GÜLLER PINAR,Akkemik Ebru,kör sevil,Ciftci Mehmet Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.8, no.3, 2018, ss.211 - 217. 10.21597/jist.458632 |
| AMA | TAŞER GÜLLER P,Akkemik E,kör s,Ciftci M Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2018; 8(3): 211 - 217. 10.21597/jist.458632 |
| Vancouver | TAŞER GÜLLER P,Akkemik E,kör s,Ciftci M Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2018; 8(3): 211 - 217. 10.21597/jist.458632 |
| IEEE | TAŞER GÜLLER P,Akkemik E,kör s,Ciftci M "Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions." Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8, ss.211 - 217, 2018. 10.21597/jist.458632 |
| ISNAD | TAŞER GÜLLER, PINAR vd. "Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions". Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi 8/3 (2018), 211-217. https://doi.org/10.21597/jist.458632 |
