Yıl: 2017 Cilt: 43 Sayı: 4 Sayfa Aralığı: 444 - 450 Metin Dili: İngilizce DOI: 10.5152/tud.2017.34101 İndeks Tarihi: 14-01-2019

Protective role of resveratrol on testicular germ cells in mice with testicular toxicity

Öz:
Objective: The aim of the present study was to investigate the possible beneficial effects of resveratrol in micesubjected to vinyl cyclohexene dieposide (VCD) -induced testicular toxicity.Material and methods: A total of thirty- six Swiss albino male mice aged 28-days were used in the presentstudy. The study was composed of two stages where mice which received or did not receive VCD (320 mg/kg/day) were administered resveratrol. The animals were assigned into control and resveratrol-treated groups inthe first stage and into groups of VCD- and VCD+resveratrol-treated groups in the second stage. At the end ofthe experiments, relative testicular weight (TW/BW) and dry/wet weight of testis (TDW/TWW) were calculated.Histological analysis by hematoxylin and eosin (H&E) staining and immunohistochemical staining byBAX and Bcl-2 were performed. Serum testosterone, LH and FSH levels were measured by a commerciallyavailable ELISA kit.Results: Resveratrol caused a dose-dependent increase in TW/BW and decrease in TDW/TWW (p<0.05).Resveratrol at a dose of 20 mg/kg resulted in an improvement in testosterone, LH and FSH levels in mice withVCD-induced testicular toxicity (p<0.001). Resveratrol also improved apoptotic index and epithelial cell heightof testicular seminipherous tubuli significantly after VCD exposure (p<0.001).Conclusion: Results of the present study suggest that resveratrol can be used as a protective and/or therapeuticagent particularly for cases with male infertility caused by testicular toxicity.
Anahtar Kelime:

Konular: Üroloji ve Nefroloji

Resveratrolün testis toksisitesi oluşturulmuş farelerde testiküler germ hücreleri üzerine koruyucu etkisi

Öz:
Amaç: Bu çalışmada amaç vinil sikloheksen diepoksid (VCD) ile testiküler toksisite oluşturulmuş farelerde resveratrolün olası faydalı etkilerini incelemektir. Gereç ve yöntemler: Çalışmada 28 günlük toplam otuz altı adet Swiss Albino erkek fare kullanıldı. Çalışma 320 mg/kg/gün VCD ile ve VCD kullanılmadan resveratrol verilerek iki aşamada oluşturuldu. İlk aşamada gruplar kontrol ve resveratrol tedavi grubu, ikinci aşamada ise VCD ve VCD+ resveratrol grupları olarak belirlendi. Tedavinin sonunda testis ağırlığı/ vücut ağırlığı oranları (TA/VA), testis yaş/kuru ağırlığı oranları (TKA/TYA) hesaplandı. BAX ve Bcl2 uygulanarak immunohistokimyasal boyama ve Hematoksilen ve Eozin (H&E) boyama uygulanarak histolojik analiz yapıldı. Serum testesteron, FSH ve LH seviyeleri uygun ticari ELİSA kit ile ölçüldü. Bulgular: Resveratrol doz bağımlı olarak TA/VA oranlarını artırmış, TKA/TYA oranlarını azaltmıştır (p<0,05). 20 mg/kg resveratrol VCD ile testis toksisitesi oluşturulan farelerde testesteron, LH, FSH seviyelerini iyileştirmiştir (p<0,001). Resveratrol ayrıca VCD maruziyetinden sonra apoptotik indeks ve testiküler seminifer tubul epitel yükseklik değerlerini de anlamlı bir şekilde düzeltmiştir (p<0,001). Sonuç: Bu sonuçlara göre resveratrol özellikle testiküler toksisite nedeni ile infertilite oluşan erkeklerde protektif ve/veya terapotik ajan olarak kullanılabilir.
Anahtar Kelime:

Konular: Üroloji ve Nefroloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Chang CC, Chang CY, Huang JP, Hung LM. Effect of resveratrol on oxidative and inflammatory stress in liver and spleen of streptozotocin- induced type 1 diabetic rats. Chin J Physiol 2012;55:192- 20. [CrossRef]
  • Kasdallah-Grissa A, Mornagui B, Aouani E, Hammami M, Gharbi N, Kamoun A, et al. Protective effect of resveratrol on ethanolinduced lipid peroxidation in rats. Alcohol Alcohol 2006;41:236- 9. [CrossRef]
  • Atessahin A, Türk G, Karahan I, Yılmaz S, Ceribası AO, Bulmus O. Lycopene preventsadriamycin-induced testicular toxicity in rats. Fertil Steril 2006;1:1216-22. [CrossRef]
  • Xin YF, You ZQ, Gao HY, Zhou GL, Chen YX, Yu J, et al. Protective effect of Lycium barbarum polysaccharides against doxorubicin-induced testicular toxicity in rats. Phytother Res 2012;26:716-21. [CrossRef]
  • Menna P, Salvatorelli E, Minotti G. Anthracycline degradation in cardiomyocytes: ajourney to oxidative survival. Chem Res Toxicol 2010;23:6-10. [CrossRef]
  • Sikka SC. Oxidative stress and role of antioxidants in normal and abnormal sperm function. Front Biosci 1996;1:78-86. [CrossRef]
  • Söylemez H, Uğraş MY, Beytur A, Oğuz F, Kuruş M, Bay Karabulut A. Evaluation of antioxidant effect of resveratrol on testicular tissue in rats that were exposed to cigarette smoke. Turk J Urol 2011;37:235-41. [CrossRef]
  • Couse JF, Korach KS. Estrogen receptor null mice: what have we learned and where will they lead us? Endocr Rev 1999;20:358- 417.
  • O’Donnell L, Robertson KM, Jones ME, Simpson ER. Estrogen and Spermatogenesis. Endocr Rev 2001;22:289-318. [CrossRef]
  • Juan ME, González-Pons E, Munuera T, Ballester J, Rodríguez- Gil JE, Planas JM. trans-Resveratrol, a natural antioxidant from grapes, increases sperm output. J Nutr 2005;135:757-60.
  • Türedi S, Yulug E, Alver A, Kutlu Ö, Kahraman C. Effects of resveratrol on doxorubicin induced testicular damage in rats. Exp Toxicol Pathol 2015;67:229-35. [CrossRef]
  • Bhat KP, Lantvit D, Christov K, Mehta RG, Moon RC, Pezzuto JM. Estrogenic and antiestrogenic properties of resveratrol in mammary tumor models. Cancer Res 2001; 61:7456-63.
  • Ke HZ, Paralkar VM, Grasser WA, Crafford DT, Qi H, Simmons HA, et al. Effects of CP-336,156, a New, Nonsteroidal Estrogen Agonist/Antagonist, on bone, Serum Cholestreol,Uterus anda Body Composition in Rat Models. Endocrinology 1998;139:2068- 76. [CrossRef]
  • Sharma P, Hug AU, Singh R. Cypermethrin-induced reproductive toxicity in the rat is prevented by resveratrol. J Hum Reprod Sci 2014;7:99-106. [CrossRef]
  • Shin JA, Oh S, Ahn JH, Park EM. Estrogen receptor-mediated resveratrol actions on blood-brain barrier of ovariectomized mice. Neurobiol Aging 2015;36:993-1006. [CrossRef]
  • Cai H, Scott E, Kholghi A, Andreadi C, Rufini A, Karmokar A, et al. Cancer chemoprevention: Evidence of a nonlinear dose response for the protective effects of resveratrol in humans and mice. Sci Transl Med 2015;7:298ra117.
  • Bhat KPL, Kosmeder JW, Pezzuto JM. Biological effects of resveratrol. Antioxid Redox Signal 2001;3:1041-64. [CrossRef]
  • Johnsen SJ. Testiculer biopsy score count-a method for registrationof spermatogesesis in human testis. Hormones 1970;1:2-25.
  • Abolaji AO, Kamdem JP, Lugokenski TH, Nascimento TK, Waczuk EP, Farombi EO, et al. Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in Drosophila melanogaster. Free Radic Biol Med 2014;82:204-5. [CrossRef]
  • Hu X, Christian P, Sipes IG, Hoyer PB. Expression and redistribution of cellular Bad, Bax, and Bcl-X(L) protein is associated with VCD-induced ovotoxicity in rats. Biol Reprod 2001;65:1489-95. [CrossRef]
  • Hoyer P, Sipes G. Devolopment of animal model for ovotoxicity using 4-vinylcyclohexene: A case study. Birth Defects Res B Dev Reprod Toxicol 2007;80:113-25. [CrossRef]
  • Frémont L. Biological effects of resveratrol. Life Sci 2000;66:663- 73. [CrossRef]
  • Royshowdhury S, Wolf G, Keilhoff D, Bagchı D, Horn T. Protection of primary glial cells by grape seed proanthocyanidin extract against nitrosative/oxidative stres. Nitric Oxide 2001;5:137-49. [CrossRef]
  • Belguendouz L, Fremont L, Gozzelino MT. Interaction of trans resveratrol with plasma proteins. Biochem Pharmacol 1998;55:811-6. [CrossRef]
  • Jiang Y, Peng T, Luo Y, Li M, Lin Y. Resveratrol reestablishes spermatogenesis after testicular injury in rats caused by 2,5-hexanedione. Chin Med J (Engl) 2008;121:204-9.
  • Revel A, Raanani H, Younglai E, Xu J, Han R, Savouret JF, et al. Resveratrol, a natural aryl hydrocarbon receptor antagonist, protects sperm from DNA damage and apoptosis caused by benzo(a) pyrene. Reprod Toxicol 2001;15:479-86. [CrossRef]
  • Hooser SB, Demerell DG, Douds DA, Hoyer P, Sipes IG. Testicular germ cell toxicity caused by vinylcyclohexene diepoxide in mice. Reprod Toxicol 1995;9:359-67. [CrossRef]
  • Shin S, Jeon JH, Park D, Jang MJ, Choi JH, Choi BH, et al. Transresveratrol relaxes the corpus cavernosum ex vivo and enhances testosterone levels and sperm quality in vivo.” Arch Pharm Res 2008;31:83-7. [CrossRef]
  • Chen YC, Nagpal MD, Stocco DM, Lin T. Effects of genistein,resveratrol,and quercetin on steroidogenesis and proliferation of MA-10 mouse Leydig tumor cells. Journal of Endocrinology 2007;192:527-37. [CrossRef]
  • Gu X, Chu Q, O’Dawyer M, Zeece M. Analysis of reveratrol in wine by capillary electrophoresis. J Cromotograph A 2000;881:478-81.
  • Ray PS, Maulik G, Cordis GA, Bertelli AAE, Das DK. The red wine antioxidant resveratrol protecs isolated at hearts from ischemia reperfusion injury. Free Radical Biol Med 1999;27:160-9. [CrossRef]
APA Özatik F, ÖZATİK O, YİĞİTASLAN S, ÇENGELLI ÜNEL Ç, Erol K (2017). Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. , 444 - 450. 10.5152/tud.2017.34101
Chicago Özatik Fikriye Yasemin,ÖZATİK ORHAN,YİĞİTASLAN Semra,ÇENGELLI ÜNEL ÇIGDEM,Erol Kevser Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. (2017): 444 - 450. 10.5152/tud.2017.34101
MLA Özatik Fikriye Yasemin,ÖZATİK ORHAN,YİĞİTASLAN Semra,ÇENGELLI ÜNEL ÇIGDEM,Erol Kevser Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. , 2017, ss.444 - 450. 10.5152/tud.2017.34101
AMA Özatik F,ÖZATİK O,YİĞİTASLAN S,ÇENGELLI ÜNEL Ç,Erol K Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. . 2017; 444 - 450. 10.5152/tud.2017.34101
Vancouver Özatik F,ÖZATİK O,YİĞİTASLAN S,ÇENGELLI ÜNEL Ç,Erol K Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. . 2017; 444 - 450. 10.5152/tud.2017.34101
IEEE Özatik F,ÖZATİK O,YİĞİTASLAN S,ÇENGELLI ÜNEL Ç,Erol K "Protective role of resveratrol on testicular germ cells in mice with testicular toxicity." , ss.444 - 450, 2017. 10.5152/tud.2017.34101
ISNAD Özatik, Fikriye Yasemin vd. "Protective role of resveratrol on testicular germ cells in mice with testicular toxicity". (2017), 444-450. https://doi.org/10.5152/tud.2017.34101
APA Özatik F, ÖZATİK O, YİĞİTASLAN S, ÇENGELLI ÜNEL Ç, Erol K (2017). Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. Turkish Journal of Urology, 43(4), 444 - 450. 10.5152/tud.2017.34101
Chicago Özatik Fikriye Yasemin,ÖZATİK ORHAN,YİĞİTASLAN Semra,ÇENGELLI ÜNEL ÇIGDEM,Erol Kevser Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. Turkish Journal of Urology 43, no.4 (2017): 444 - 450. 10.5152/tud.2017.34101
MLA Özatik Fikriye Yasemin,ÖZATİK ORHAN,YİĞİTASLAN Semra,ÇENGELLI ÜNEL ÇIGDEM,Erol Kevser Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. Turkish Journal of Urology, vol.43, no.4, 2017, ss.444 - 450. 10.5152/tud.2017.34101
AMA Özatik F,ÖZATİK O,YİĞİTASLAN S,ÇENGELLI ÜNEL Ç,Erol K Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. Turkish Journal of Urology. 2017; 43(4): 444 - 450. 10.5152/tud.2017.34101
Vancouver Özatik F,ÖZATİK O,YİĞİTASLAN S,ÇENGELLI ÜNEL Ç,Erol K Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. Turkish Journal of Urology. 2017; 43(4): 444 - 450. 10.5152/tud.2017.34101
IEEE Özatik F,ÖZATİK O,YİĞİTASLAN S,ÇENGELLI ÜNEL Ç,Erol K "Protective role of resveratrol on testicular germ cells in mice with testicular toxicity." Turkish Journal of Urology, 43, ss.444 - 450, 2017. 10.5152/tud.2017.34101
ISNAD Özatik, Fikriye Yasemin vd. "Protective role of resveratrol on testicular germ cells in mice with testicular toxicity". Turkish Journal of Urology 43/4 (2017), 444-450. https://doi.org/10.5152/tud.2017.34101