(Department of Pharmacology, Ahi Evran University School of Medicine, Kırsehir, Turkey)
(Department of Histology and Embriyology, Ahi Evran University School of Medicine, Kırsehir, Turkey)
(Department of Pharmacology, Eskişehir Osmangazi University School of Medicine, Eskişehir, Turkey)
(Department of Pharmacology, Eskişehir Osmangazi University School of Medicine, Eskişehir,Turkey)
(Department of Pharmacology, Eskişehir Osmangazi University School of Medicine, Eskişehir, Turkey)
Yıl: 2017Cilt: 43Sayı: 4ISSN: 2149-3235 / 2149-3057Sayfa Aralığı: 444 - 450İngilizce

93 0
Protective role of resveratrol on testicular germ cells in mice with testicular toxicity
Objective: The aim of the present study was to investigate the possible beneficial effects of resveratrol in mice subjected to vinyl cyclohexene dieposide (VCD) -induced testicular toxicity. Material and methods: A total of thirty- six Swiss albino male mice aged 28-days were used in the present study. The study was composed of two stages where mice which received or did not receive VCD (320 mg/kg/ day) were administered resveratrol. The animals were assigned into control and resveratrol-treated groups in the first stage and into groups of VCD- and VCD+resveratrol-treated groups in the second stage. At the end of the experiments, relative testicular weight (TW/BW) and dry/wet weight of testis (TDW/TWW) were calculated. Histological analysis by hematoxylin and eosin (H&E) staining and immunohistochemical staining by BAX and Bcl-2 were performed. Serum testosterone, LH and FSH levels were measured by a commercially available ELISA kit. Results: Resveratrol caused a dose-dependent increase in TW/BW and decrease in TDW/TWW (p<0.05). Resveratrol at a dose of 20 mg/kg resulted in an improvement in testosterone, LH and FSH levels in mice with VCD-induced testicular toxicity (p<0.001). Resveratrol also improved apoptotic index and epithelial cell height of testicular seminipherous tubuli significantly after VCD exposure (p<0.001). Conclusion: Results of the present study suggest that resveratrol can be used as a protective and/or therapeutic agent particularly for cases with male infertility caused by testicular toxicity.
Fen > Tıp > Üroloji ve Nefroloji
DergiAraştırma MakalesiErişime Açık
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