Yıl: 2017 Cilt: 21 Sayı: 1 Sayfa Aralığı: 121 - 126 Metin Dili: İngilizce DOI: 10.12991/marupj.259889 İndeks Tarihi: 17-01-2019

Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines

Öz:
Laurocerasus officinalis Roem. (Prunus laurocerasus l.), also locally known as “karayemis or taflan”, is a popular black summer fruit belonging to the genus Prunus and the family Rosaceae. L. officinalis exhibits numerous biological activities, such as anti-inflammatory, antinociceptive, antioxidant, neuroprotective, and antidiabetic effects. The aim of this study was to determine the total phenolic content and cytotoxic effect of dimethyl sulfoxide extract of L. officinalis (DEl). The total phenolic content and cytotoxic effect of DEl were investigated using the Folin-ciocalteu method and thiazolyl blue tetrazolium bromide (MTT) assay, respectively. Total phenolic content value was 33.7±0.13 mg gallic acid equivalent per g sample. DEl exhibited selective cytotoxic effects against four human cancer (lung, colon, liver, and cervix) cell lines. The most selective cytotoxic effect of the extract was observed in colon cancer cells (Ic50=265.2±8.1 µg/ml) compared to normal fibroblast cells. These data demonstrate that L. officinalis extract exhibits selective cytotoxicity towards some cancer cells. Further studies are now needed to clarify the molecules involved and their mechanisms.
Anahtar Kelime:

Konular: Farmakoloji ve Eczacılık

Laurocerasus officinalis Ekstraktının İnsan Kanser Hücre Serileri Üzerindeki Sitotoksik Etkisi

Öz:
Laurocerasus officinalis Roem. (Prunus laurocerasus l.), yerel olarak karayemiş veya taflan olarak da bilinir, Prunus cinsi ve Rosaceae familyasına mensup, koyu renkli bir yaz meyvesidir. L. officinalis, anti-inflamatuvar, antinosiseptif, antioksidan, nöroprotektif ve antidiyabetik gibi çeşitli biyolojik aktiviteler göstermektedir. Bu çalışmanın amacı, L. officinalis’in dimetil sülfoksitli ekstraktının antioksidan ve sitotoksik etkilerini belirlemektir. Ekstraktın toplam polifenol içeriği Folin ciocalteu metodu, sitotoksik etkinliği ise MTT yöntemiyle belirlendi. Ekstraktın toplam polifenol içeriği 33.7±0.13 mg gallik asit ekivalanı/g örnek olarak bulundu. Ekstrakt akciğer, kolon, karaciğer ve serviks kanser hücre serilerinde seçici bir sitotoksik etki gösterdi. normal fibroblast hücreleri ile karşılaştırıldığında en etkili seçici sitotoksik etkinin kolon kanseri hücrelerinde olduğu belirlendi (Ic50=265.2±8.1 µg/ml). Sonuçlar L. officinalis ekstraktının bazı kanser hücre serilerinde seçici bir sitotoksik etki gösterebildiğini ortaya koymaktadır. Ekstrakttaki etken molekülleri ve bunların etki mekanizmalarını belirleyebilmek için daha ileri çalışmalara ihtiyaç vardır.
Anahtar Kelime:

Konular: Farmakoloji ve Eczacılık
Belge Türü: Makale Makale Türü: Derleme Erişim Türü: Erişime Açık
  • Jaganathan SK, Supriyanto E, Mandal M. Events associated with apoptotic effect of p-coumaric acid in HcT-15 colon cancer cells. World J Gastroenterol 2013; 19: 7726-34.
  • Feng R, lu Y, Bowman Yl, Qian Y, castranova V, Ding M. Inhibition of activator protein-1, nF-κB, and MAPKs and induction of phase 2 detoxifying enzyme activity by chlorogenic acid. J Biol chem 2005; 290: 27888-95.
  • Kampa M, Alexaki VI, notas G, nifli AP, nistikaki A, Hatzoglou A, et al. Antiproliferative and apoptotic effects of selective phenolic acids on T47D human breast cancer cells: potential mechanisms of action. Breast cancer Res 2003; 6: 63-74.
  • li An, li S, zhang YJ, Xu XR, chen YM, li HB. Resources and biological activities of natural polyphenols. nutrients 2014; 6: 6020-47.
  • Han Xz, Shen T, lou HX. Dietary polyphenols and their biological significance. Int J Mol Sci 2007; 8: 950-88.
  • Surh YJ. cancer chemoprevention with dietary phytochemicals. nat Rev cancer 2003; 3: 768-80.
  • Rice-Evans cA, Miller nJ, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci 1997; 2: 152-9.
  • Sunaga n, Hiraishi K, Ishizuka T, Kaira K, Iwasaki Y, Jimma F, et al. MK615, a compound extract from the Japanese apricot “Prunus mume” inhibits in vitro cell growth and interleukin-8 expression in non-small cell lung cancer cells. J cancer Sci Ther 2011; S11: 002.
  • Jeong JT, Moon JH, Park KH, Shin cS. Isolation and characterization of a new compound from Prunus mume fruit that inhibits cancer cells. J Agric Food chem 2006; 54: 2123-8.
  • Adachi M, Suzuki Y, Mizuta T, Osawa T, Adachi T, Osaka K, et al. The “Prunus mume Sieb. et zucc” (ume) is a rich natural source of novel anti-cancer substance. Int J Food Prop 2007; 10: 375-84.
  • Fujii T, Ikami T, Xu JW, Ikeda KJ. Prune extract (Prunus domestica l.) suppresses the proliferation and induces the apoptosis of human colon carcinoma caco-2. nutr Sci Vitaminol 2006; 52: 389-91.
  • Galati G, O’Brien PJ. Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radic Biol Med 2004; 37: 287-303.
  • lee BB, cha MR, Kim SY, Park E, Park HR, lee Sc. Antioxidative and anticancer activity of extracts of cherry (Prunus serrulata var. spontanea) blossoms. Plant Foods Hum nutr 2007; 62: 79-84.
  • Orhan IE, Akkol EK. Estimation of neuroprotective effects of Laurocerasus officinalis Roem. (cherry laurel) by in vitro methods. Food Res Int 2011; 44: 818-22.
  • Vinson JA, Su X, zubik l, Bose P. Phenol antioxidant quality in food: fruits. J Agr and Food chem 2001; 49: 5315-21.
  • Dai J, Mumper RJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules 2010; 15: 7313-52.
  • Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 55-63.
  • Slinkard K, Singleton Vl. Total phenol analyses: automation and comparison with manual methods. Am J Enol Vitic 1977; 28: 49-55.
  • Karahalil FY, Sahin H. Phenolic composition and antioxidant capacity of cherry laurel (Laurocerasus officinalis Roem.) sampled from Trabzon region, Turkey. Afr J Biotechnol 2011; 10: 16293-9.
  • Alasalvar c, Al-Farsi M, Shahidi F. compositional characteristics and antioxidant components of cherry laurel varieties and pekmez. J Food Sci 2005; 70: 47-52.
  • Turan I, Demir S, Misir S, Kilinc K, Mentese A, Aliyazicioglu Y, Deger O. cytotoxic effect of Turkish propolis on liver, colon, breast, cervix and prostate cancer cell lines. Trop J Pharm Res 2015; 14: 777-82.
  • noratto G, Porter W, Byrne D, cisneros-zevallos c. Polyphenolics from peach (Prunus persica var. Rich lady) inhibit tumor growth and metastasis of MDA-MB-435 breast cancer cells in vivo. J nutr Biochem 2014; 25: 796-800.
  • Poongodi T, Srikanth R, lalitha G. Phytochemistry, Gc-MS analysis and in vitro cytotoxic activity of Prunus angustifolia leaves against McF-7 breast cancer cell line. WJPPS 2015; 4: 1489-99.
  • nabende Pn, Karanja SM, Mwatha JK, Wachira SW. Anti-proliferative activity of Prunus africana, Warburgia stuhlmannii and Maytenus senegalensis extracts in breast and colon cancer cell lines. Eur J Med Plants 2015; 5: 366-76.
  • Demir S, Aliyazicioglu Y, Turan I, Misir S, Mentese A, Yaman SO, Akbulut K, Kilinc K, Deger O. Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line. nutr cancer 2016; 68: 165-72.
  • Gottesman MM. Mechanism of cancer drug resistance. Annu Rev Med 2002; 53: 615-27.
  • Huang WY, cai Yz. natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention. nutr cancer 2010; 62: 1-20.
  • Reddy l, Odhav B, Bhoola KD. natural products for cancer prevention: a global perspective. Pharmacol Ther 2003; 99: 1-13.
  • Senayli A, Sahin A, Senayli Y, Elmastas M. Evaluation the anti-diabetic activity of cherry laurel (Laurocerasus officinalis). Open conf Proc J 2012; 3: 8-12.
  • Akbulut S, Bayramoglu MM. Reflections of socio-economic and demographic structure of urban and rural on the use of medicinal and aromatic plants: The sample of Trabzon province. Ethno Med 2014; 8: 89-100.
  • Karabegovic IT, Stojicevic SS, Velickovic DT, nikolic nc, lazic Ml. Optimization of microwave-assisted extraction of cherry laurel fruit. Separ Sci Technol 2014; 49: 416-23.
  • celep E, Aydin A, Yesilada E. A comparative study on the in vitro antioxidant potentials of three edible fruits: cornelian cherry, Japanese persimmon and cherry laurel. Food chem Toxicol 2012; 50: 3329-35.
  • liyana-Pathirana cM, Shahidi F, Alasalvar c. Antioxidant activity of cherry laurel fruit (Laurocerasus officinalis Roem.) and its concentrated juice. Food chem 2006; 99: 121-8.
  • Kolayli S, Kucuk M, Duran c, candan F, Dincer B. chemical and antioxidant properties of Laurocerasus officinalis Roem. (cherry laurel) fruit grown in the Black Sea region. J Agric Food chem 2003; 51: 7489-94.
  • Erdemoglu n, Kupeli E, Yesilada E. Anti-inflammatory and antinociceptive activity assessment of plants used as remedy in Turkish folk medicine. J Ethnopharmacol 2003; 89: 123-9.
APA Demir S, Turan İ, DEMİR F, SEYAZ M, Aliyazıcıoğlu Y (2017). Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. , 121 - 126. 10.12991/marupj.259889
Chicago Demir Selim,Turan İbrahim,DEMİR Feyza,SEYAZ MEHTAP,Aliyazıcıoğlu Yüksel Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. (2017): 121 - 126. 10.12991/marupj.259889
MLA Demir Selim,Turan İbrahim,DEMİR Feyza,SEYAZ MEHTAP,Aliyazıcıoğlu Yüksel Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. , 2017, ss.121 - 126. 10.12991/marupj.259889
AMA Demir S,Turan İ,DEMİR F,SEYAZ M,Aliyazıcıoğlu Y Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. . 2017; 121 - 126. 10.12991/marupj.259889
Vancouver Demir S,Turan İ,DEMİR F,SEYAZ M,Aliyazıcıoğlu Y Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. . 2017; 121 - 126. 10.12991/marupj.259889
IEEE Demir S,Turan İ,DEMİR F,SEYAZ M,Aliyazıcıoğlu Y "Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines." , ss.121 - 126, 2017. 10.12991/marupj.259889
ISNAD Demir, Selim vd. "Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines". (2017), 121-126. https://doi.org/10.12991/marupj.259889
APA Demir S, Turan İ, DEMİR F, SEYAZ M, Aliyazıcıoğlu Y (2017). Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. Marmara Pharmaceutical Journal, 21(1), 121 - 126. 10.12991/marupj.259889
Chicago Demir Selim,Turan İbrahim,DEMİR Feyza,SEYAZ MEHTAP,Aliyazıcıoğlu Yüksel Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. Marmara Pharmaceutical Journal 21, no.1 (2017): 121 - 126. 10.12991/marupj.259889
MLA Demir Selim,Turan İbrahim,DEMİR Feyza,SEYAZ MEHTAP,Aliyazıcıoğlu Yüksel Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. Marmara Pharmaceutical Journal, vol.21, no.1, 2017, ss.121 - 126. 10.12991/marupj.259889
AMA Demir S,Turan İ,DEMİR F,SEYAZ M,Aliyazıcıoğlu Y Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. Marmara Pharmaceutical Journal. 2017; 21(1): 121 - 126. 10.12991/marupj.259889
Vancouver Demir S,Turan İ,DEMİR F,SEYAZ M,Aliyazıcıoğlu Y Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines. Marmara Pharmaceutical Journal. 2017; 21(1): 121 - 126. 10.12991/marupj.259889
IEEE Demir S,Turan İ,DEMİR F,SEYAZ M,Aliyazıcıoğlu Y "Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines." Marmara Pharmaceutical Journal, 21, ss.121 - 126, 2017. 10.12991/marupj.259889
ISNAD Demir, Selim vd. "Cytotoxic Effect of Laurocerasus officinalis Extract on Human Cancer Cell Lines". Marmara Pharmaceutical Journal 21/1 (2017), 121-126. https://doi.org/10.12991/marupj.259889