Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells

ÖZEŞ, Osman Nidai; AKÇA, Hakan

Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells

Hakan AKÇA, (Akdeniz Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Antalya, Türkiye)

Osman Nidai ÖZEŞ (Girilmedi)

Turkish Journal of Biology

6 0

Yıl: 2002 Cilt: 26 Sayı: 3 Sayfa Aralığı: 145 - 150 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells

Öz:

Servikal karsinoma kadınlarda yüksek ölüm oranı gösteren bir kanser tipidir. Bu araştırmada, hidroksiüre’nin insan servikal karsinoma hücre dizisi, HeLa, üzerindeki sitotoksik, antiproliferatif ve apoptosis indukleyici etkileri araştırılmıştır. Hidroksiüre’nin doz bağımlı olarak sitotoksik ve antiproliferatif etki gösterdiği saptanmıştır. Ayrıca, konsantrasyona bağlı olarak hidroksiüre'nin hücre içi p53 ve p21 seviyelerini artırdığı ve DNA fragmentasyonunu induklediği saptanmıştır. Sonuç olarak hidroksiüre’nin antiproliferatif, sitotoksik ve apoptosisi indukleyici etkilerinin p53 ve onun transkripsiyonel hedefi, p21’in indüksiyonu aracılığı ile bağlantılı olabilecei tartışılmıştır.

Anahtar Kelime: servikal kanser sitotoksik bileşikler DNA HeLa hücreleri programlanmış hücre ölümü insan hastalıkları uyarılma antineoplastik ajanlar hidroksikarbamit

Konular: Biyoloji

p53 birikimi ve apoptosisin insan servrikal karsinoma hücrelerinde hidroksiüre tarafından indüklenmesi

Öz:

Cervical carcinoma is one type of cancer with a high mortality rate in women. In this research, we investigated the cytotoxic, antiproliferative and apoptosis-inducing effects of hydroxyurea on a human cervical carcinoma cell line, HeLa. We found that hydroxyurea has cytotoxic and antiproliferative effects on HeLa cells in a dose-dependent manner. We also found that hydroxyurea can increase cellular levels of p53 and p21, and induce DNA fragmentation in a dose-dependent manner. We concluded that the observed antiproliferative, cytotoxic and apoptosis inducing activities of hydroxyurea might be mediated by the induction of p53 and its transcriptional target, p21

Anahtar Kelime: apoptosis human diseases induction antineoplastic agents hydroxycarbamide cervical cancer cytotoxic compounds DNA HeLa cells

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	AKÇA H, ÖZEŞ O (2002). Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells. , 145 - 150.
Chicago	AKÇA Hakan,ÖZEŞ Osman Nidai Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells. (2002): 145 - 150.
MLA	AKÇA Hakan,ÖZEŞ Osman Nidai Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells. , 2002, ss.145 - 150.
AMA	AKÇA H,ÖZEŞ O Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells. . 2002; 145 - 150.
Vancouver	AKÇA H,ÖZEŞ O Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells. . 2002; 145 - 150.
IEEE	AKÇA H,ÖZEŞ O "Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells." , ss.145 - 150, 2002.
ISNAD	AKÇA, Hakan - ÖZEŞ, Osman Nidai. "Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells". (2002), 145-150.

APA	AKÇA H, ÖZEŞ O (2002). Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells. Turkish Journal of Biology, 26(3), 145 - 150.
Chicago	AKÇA Hakan,ÖZEŞ Osman Nidai Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells. Turkish Journal of Biology 26, no.3 (2002): 145 - 150.
MLA	AKÇA Hakan,ÖZEŞ Osman Nidai Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells. Turkish Journal of Biology, vol.26, no.3, 2002, ss.145 - 150.
AMA	AKÇA H,ÖZEŞ O Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells. Turkish Journal of Biology. 2002; 26(3): 145 - 150.
Vancouver	AKÇA H,ÖZEŞ O Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells. Turkish Journal of Biology. 2002; 26(3): 145 - 150.
IEEE	AKÇA H,ÖZEŞ O "Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells." Turkish Journal of Biology, 26, ss.145 - 150, 2002.
ISNAD	AKÇA, Hakan - ÖZEŞ, Osman Nidai. "Hydroxyurea induces p53 accumulation and apoptosis in human cervical carcinoma cells". Turkish Journal of Biology 26/3 (2002), 145-150.