Yıl: 2018 Cilt: 7 Sayı: 1 Sayfa Aralığı: 12 - 19 Metin Dili: İngilizce DOI: 10.18036/aubtdc.306292 İndeks Tarihi: 01-02-2019

LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA

Öz:
Lipases are widely distributed in nature and catalyze the hydrolysis of mono, di and tri acyl glycerols to liberate free fatty acids and glycerol. Various microorganisms produce lipases either alone or together with esterase. Lipases form an important group of relevant enzymes and they were used in a variety of biotechnological fields such as food, pharmaceutical, detergent, textile and cosmetic industries. Lipases from lactic acid bacteria may contribute in the development of flavors in foods especially meat products. The goal of the present study is to investigate the ability to produce lipolytic enzymes lactic acid bacteria strains, which is selected as potential probiotics and isolated on pastırma samples in our laboratory to determinate the possibilities of using as starter cultures in pastırma production. Lipolytic activity of lactic acid bacteria isolated from pastırma has not been investigated up to now. The plate assay is performed to screening lipolytic activity of lactic acid bacteria isolates. In the study, three different growth medium tried and each medium contain tributyrin as a substrate. Using these methods, 50 strains of lactic acid bacteria isolated from Turkish pastırma are screened for the presence of lipolytic activity. Because of the analysis, the lipolytic activity is observed in 25 tested strains. Lactobacillus plantarum have highest lipolytic activity.
Anahtar Kelime:

PASTIRMADAN İZOLE EDİLEN LAKTİK ASİT BAKTERİLERİNİN LİPOLİTİK AKTİVİTESİ

Öz:
Lipazlar doğada yaygın olarak bulunmaktadır ve mono, di, tri asil giliserollerin serbest yağ asitleri ve gliserole hidrolizini katalizlemektedirler. Çeşitli mikroorganizmalar lipazları tek başına ya da esterazlar ile birlikte üretmektedirler. Lipazlar ilgili enzimlerin önemli bir grubunu oluşturmaktadır ve gıda, ilaç, deterjan, tekstil ve kozmetik endüstrisi gibi çeşitli biyoteknolojik alanlarda kullanılmaktadırlar. Laktik asit bakteri lipazları gıdalarda özellikle de et ürünlerinde lezzet gelişimine katkı sağlayabilmektedir. Bu çalışmanın amacı, pastırma üretiminde starter kültür olarak kullanımlarını belirlemek için laboratuvarımızda pastırma örneklerinden izole edilmiş ve potansiyel probiyotik olarak seçilmiş laktik asit bakteri strainlerinin lipolitik enzimleri üretme yeteneklerini incelemektir. Pastırmadan izole edilen laktik asit bakterilerinin lipolitik aktivitesi şimdiye kadar araştırılmamıştır. Laktik asit bakteri izolatlarının lipolitik aktivitesinin taranması için plate analizleri gerçekleştirilmiştir. Çalışmada, 3 farklı gelişim ortamı denenmiştir ve her gelişim ortamı substrat olarak tributrin içermektedir. Bu yöntemler kullanılarak pastırmadan izole edilen 50 laktik asit bakteri straini lipolitik aktivite açısından taranmıştır. Analizler sonucunda 25 test straininde lipolitik aktivite gözlemlenmiştir. Lactobacillus plantarum en yüksek lipolitik aktiviteye sahiptir
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Dincer E, KIVANÇ M (2018). LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA. , 12 - 19. 10.18036/aubtdc.306292
Chicago Dincer Emine,KIVANÇ Merih LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA. (2018): 12 - 19. 10.18036/aubtdc.306292
MLA Dincer Emine,KIVANÇ Merih LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA. , 2018, ss.12 - 19. 10.18036/aubtdc.306292
AMA Dincer E,KIVANÇ M LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA. . 2018; 12 - 19. 10.18036/aubtdc.306292
Vancouver Dincer E,KIVANÇ M LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA. . 2018; 12 - 19. 10.18036/aubtdc.306292
IEEE Dincer E,KIVANÇ M "LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA." , ss.12 - 19, 2018. 10.18036/aubtdc.306292
ISNAD Dincer, Emine - KIVANÇ, Merih. "LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA". (2018), 12-19. https://doi.org/10.18036/aubtdc.306292
APA Dincer E, KIVANÇ M (2018). LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji, 7(1), 12 - 19. 10.18036/aubtdc.306292
Chicago Dincer Emine,KIVANÇ Merih LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji 7, no.1 (2018): 12 - 19. 10.18036/aubtdc.306292
MLA Dincer Emine,KIVANÇ Merih LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji, vol.7, no.1, 2018, ss.12 - 19. 10.18036/aubtdc.306292
AMA Dincer E,KIVANÇ M LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji. 2018; 7(1): 12 - 19. 10.18036/aubtdc.306292
Vancouver Dincer E,KIVANÇ M LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji. 2018; 7(1): 12 - 19. 10.18036/aubtdc.306292
IEEE Dincer E,KIVANÇ M "LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA." Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji, 7, ss.12 - 19, 2018. 10.18036/aubtdc.306292
ISNAD Dincer, Emine - KIVANÇ, Merih. "LIPOLYTIC ACTIVITY OF LACTIC ACID BACTERIA ISOLATED FROM TURKISH PASTIRMA". Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji 7/1 (2018), 12-19. https://doi.org/10.18036/aubtdc.306292