The Early Chalcolithic 2 (Gülpınar II) and Middle Chalcolithic period (Gülpınar III) settlements identified at Smintheion (Sanctuary of Apollo Smintheus) in the southwestern corner of the modern Biga Peninsula (ancient Troad) in north-western Anatolia have provided important evidence about textile production of Anatolian Early and Middle Chalcolithic societies. Several types of spindle whorls, loom weights, spools and pot bases with negative impressions of woven textiles, mats, and baskets are accepted as indirect evidence of textile production in prehistoric Anatolia. Nevertheless, the direct evidence of textile production is scarce and only represented by carbonized textile remains which were rarely found in archeological contexts. The excavations conducted at Chalcolithic settlements of Gülpınar have resulted in identification of clay objects identified as spindle whorls, pot bases with negative impressions of woven textiles, mats, and baskets mostly related to the textile production. According to these findings it is understood that textile production including mat making was an important craft activity at the settlement. Thirty-nine clay objects including referred to as spindle whorls were catalogued from the 2004-2014 excavations. Spindle whorls at Gülpınar shows that they were represented in six different types. The most common forms are biconical, hemispherical and truncated conical types. The spindle whorls in various weights and shapes demonstrate the possibility of different types of width of threads, were produced for weaving. In addition, the spindle whorls are also evaluated with ethnographic examples based on traditional weaving techniques in Gülpınar and its vicinity. It is hoped that these tools will contribute to our understanding of long-term conventions in textile production of prehistoric people and in the functional use of these objects.