Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis
Yıl: 2018 Cilt: 19 Sayı: 4 Sayfa Aralığı: 289 - 295 Metin Dili: İngilizce DOI: 10.4274/meandros.08370 İndeks Tarihi: 06-09-2019
Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis
Öz: Objective: The aim of this study was to evaluate bacterial reduction after using different irrigants, lasers and photodynamic therapy (PDT) in root canals infected by Enterococcus faecalis . Materials and Methods: Seventy human maxillary incisors were used in this study. After endodontic preparation, sixty teeth were infected by 20 µl E. faecalis (ATCC 29212) and specimens were incubated for 72 hours. Groups were formed according to the irrigation protocol used: group 1: negative control, group 2: positive control, group 3: 10 mL 2% chlorhexidine (CHX), group 4: 10 mL 5% NaOCl, group 5: diode laser, group 6: Er:YAG laser, and group 7: indocyanine green (ICG)-diode laser (PDT). After disinfection procedures the samples isolated from root canals were placed into blood agar, incubated at 37 °C for 24 h and then were counted for colony-forming units. One-Way ANOVA was used for the statistical evaluation of data. For multiple comparisons, Tamhane’s T2 test was used. Results: All investigated disinfection procedures had a significant reduction in the bacterial population (p<0.001). CHX, NaOCl and ICG-diode laser groups were equally effective in reducing E. faecalis populations (p>0.05) and statistically more effective than both laser groups (p<0.05). Conclusion: Promising results were obtained by using PDT with ICG. This alternative disinfection method found effective on E. faecalis.
Anahtar Kelime: Konular:
Enterococcus Faecalis ile Enfekte Edilmiş Kök Kanallarında Farklı İrrigasyon Solüsyonlarının, Lazerlerin ve İndosiyanin Yeşili ile Fotodinamik Terapinin Antibakteriyel Etkinliği
Öz: Amaç: Bu çalışmanın amacı, Enterococcus faecalis ile enfekte edilmiş kök kanallarında farklı irrigasyon solüsyonları, lazerler ve fotodinamik tedavi (FDT) kullandıktan sonra bakteri sayısındaki azalmayı değerlendirmektir. Gereç ve Yöntemler: Bu çalışmada yetmiş adet insan üst çene kesici dişi kullanıldı. Kök kanal preparasyonundan sonra altmış diş 20 μL E. faecalis (ATCC 29212) ile enfekte edildi ve numuneler 72 saat inkübasyona bırakıldı. Gruplar, kullanılan dezenfeksiyon protokolüne göre oluşturuldu: grup 1: Negatif kontrol, grup 2: Pozitif kontrol, grup 3: 10 mL %2 klorheksidin (KH), grup 4: 10 mL %5 NaOCl, grup 5: Diyot lazer, grup 6: Er:YAG lazer ve grup 7: İndosiyanin yeşili (İSY)-diyot lazer (FDT). Dezenfeksiyon prosedürlerinden sonra kök kanallarından izole edilen numuneler kanlı agara yerleştirildi, 37 °C’de 24 saat inkübe edildi ve daha sonra koloni oluşturan birimler sayıldı. Verilerin istatistiksel değerlendirilmesi için tek yönlü varyans analizi, çoklu karşılaştırmalar için Tamhane’nin T2 testi kullanıldı. Bulgular: İncelenen tüm dezenfeksiyon prosedürleri bakteri popülasyonunda belirgin bir azalmaya neden olmuştur (p<0,001). KH, NaOCl ve İSY-diyot lazer grupları, E. faecalis popülasyonlarının azaltılmasında eşit derecede (p>0,05), her iki lazer grubundan ise istatistiksel olarak anlamlı seviyede daha etkindi (p<0,05). Sonuç: İSY’nin kullanıldığı FDT’de umut verici sonuçlar elde edildi. Bu alternatif dezenfeksiyon yöntemi E. faecalis üzerinde etkili bulundu.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Xhevdet A, Stubljar D, Kriznar I, Jukic T, Skvarc M, Veranic P, et al. The disinfecting efficacy of root canals with laser photodynamic therapy. J Lasers Med Sci 2014; 5: 19-26.
- Vivacqua-Gomes N, Gurgel-Filho ED, Gomes BP, Ferraz CC, Zaia AA, Souza-Filho FJ. Recovery of Enterococcus faecalis after single- or multiple-visit root canal treatments carried out in infected teeth ex vivo. Int Endod J 2005; 38: 697-704.
- Rald DP, Lage-Marques JL. In vitro evaluation of the effects of the interaction between irrigating solutions, intracanal medication and Er:YAG laser in dentin permeability of the endodontic system. Braz Oral Res 2003; 17: 278-85.
- Zehnder M. Root canal irrigants. J Endod 2006; 32: 389-98.
- Basrani B, Lemonie C. Chlorhexidine gluconate. Aust Endod J 2005; 31: 48-52.
- Chavez de Paz LE. Redefining the persistent infection in root canals: possible role of biofilm communities. J Endod 2007; 33: 652-62.
- Pinheiro ET, Gomes BP, Ferraz CC, Sousa EL, Teixeira FB, SouzaFilho FJ. Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J 2003; 36: 1-11.
- Haapasalo M, Orstavik D. In vitro infection and disinfection of dentinal tubules. J Dent Res 1987; 66: 1375-79.
- Ok E, Adanir N, Hakki S. Comparison of cytotoxicity of various concentrations origanum extract solution with 2% chlorhexidine gluconate and 5.25% sodium hypochlorite. Eur J Dent 2015; 9: 6-10.
- Zan R, Hubbezoglu I, Sumer Z, Tunc T, Tanalp J. Antibacterial effects of two different types of laser and aqueous ozone against Enterococcus faecalis in root canals. Photomed Laser Surg 2013; 31: 150-54.
- Olivi G, DiVito E, Peters O, Kaitsas V, Angiero F, Signore A, Benedicenti S. Disinfection efficacy of photon-induced photoacoustic streaming on root canals infected with Enterococcus faecalis: an ex vivo study. J Am Dent Assoc 2014; 145: 843-48.
- Silva EJ, Coutinho-Filho WP, Andrade AO, Herrera DR, CoutinhoFilho TS, Krebs RL. Evaluation of photodynamic therapy using a diode laser and different photosensitizers against enterococcus faecalis. Acta Odontol Latinoam 2014; 27: 63-65.
- Guneser MB, Arslan D, Usumez A. Tissue dissolution ability of sodium hypochlorite activated by photon-initiated photoacoustic streaming technique. J Endod 2015; 41: 729-32.
- Gursoy H, Ozcakir-Tomruk C, Tanalp J, Yilmaz S. Photodynamic therapy in dentistry: a literature review. Clin Oral Investig 2013; 17: 1113-25.
- Chrepa V, Kotsakis GA, Pagonis TC, Hargreaves KM. The effect of photodynamic therapy in root canal disinfection: a systematic review. J Endod 2014; 40: 891-98.
- Shrestha A, Kishen A. Antibiofilm efficacy of photosensitizerfunctionalized bioactive nanoparticles on multispecies biofilm. J Endod 2014; 40: 1604-10.
- Sheth RA, Heidari P, Esfahani SA, Wood BJ, Mahmood U. Interventional optical molecular imaging guidance during percutaneous biopsy. Radiology 2014; 271: 770-77.
- Samorani D, Fogacci T, Panzini I, Frisoni G, Accardi FG, Ricci M, et al. The use of indocyanine green to detect sentinel nodes in breast cancer: a prospective study. Eur J Surg Oncol 2015; 41: 64-70.
- Parker S. The use of diffuse laser photonic energy and indocyanine green photosensitiser as an adjunct to periodontal therapy. Br Dent J 2013; 215: 167-71.
- Nagahara A, Mitani A, Fukuda M, Yamamoto H, Tahara K, Morita I, et al. Antimicrobial photodynamic therapy using a diode laser with a potential new photosensitizer, indocyanine greenloaded nanospheres, may be effective for the clearance of Porphyromonas gingivalis. J Periodontal Res 2013; 48: 591-99.
- Borchers R. Photodynamic therapy with the new active ingredient Perio Green. Laser 2013; 5: 26-8.
- Rahimi S, Janani M, Lotfi M, Shahi S, Aghbali A, Vahid Pakdel M, Salem Milani A, Ghasemi N. A review of antibacterial agents in endodontic treatment. Iran Endod J 2014; 9: 161-68.
- Baker NE, Liewehr FR, Buxton TB, Joyce AP. Antibacterial efficacy of calcium hydroxide, iodine potassium iodide, betadine, and betadine scrub with and without surfactant against E faecalis in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2004; 98: 359-64.
- Kustarci A, Sumer Z, Altunbas D, Kosum S. Bactericidal effect of KTP laser irradiation against Enterococcus faecalis compared with gaseous ozone: an ex vivo study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2009; 107: 73-79.
- Neelakantan P, Cheng CQ, Mohanraj R, Sriraman P, Subbarao C, Sharma S. Antibiofilm activity of three irrigation protocols activated by ultrasonic, diode laser or Er:YAG laser in vitro. Int Endod J 2015; 48: 602-10.
- Kayaoglu G, Omurlu H, Akca G, Gurel M, Gencay O, Sorkun K, et al. Antibacterial activity of Propolis versus conventional endodontic disinfectants against Enterococcus faecalis in infected dentinal tubules. J Endod 2011; 37: 376-81.
- Prado M, Silva EJ, Duque TM, Zaia AA, Ferraz CC, Almeida JF, et al. Antimicrobial and cytotoxic effects of phosphoric acid solution compared to other root canal irrigants. J Appl Oral Sci 2015; 23: 158-63.
- Siqueira JF Jr., Rocas IN. Optimising single-visit disinfection with supplementary approaches: a quest for predictability. Aust Endod J 2011; 37: 92-98.
- Bonsor SJ, Nichol R, Reid TM, Pearson GJ. An alternative regimen for root canal disinfection. Br Dent J 2006; 201: 101-05.
- Fimple JL, Fontana CR, Foschi F, Ruggiero K, Song X, Pagonis TC, et al. Photodynamic treatment of endodontic polymicrobial infection in vitro. J Endod 2008; 34: 728-34.
- Garcez AS, Nunez SC, Hamblim MR, Suzuki H, Ribeiro MS. Photodynamic therapy associated with conventional endodontic treatment in patients with antibiotic-resistant microflora: a preliminary report. J Endod 2010; 36: 1463-66.
- Pagonis TC, Chen J, Fontana CR, Devalapally H, Ruggiero K, Song X, et al. Nanoparticle-based endodontic antimicrobial photodynamic therapy. J Endod 2010; 36: 322-28.
- Rios A, He J, Glickman GN, Spears R, Schneiderman ED, Honeyman AL. Evaluation of photodynamic therapy using a lightemitting diode lamp against Enterococcus faecalis in extracted human teeth. J Endod 2011; 37: 856-59.
- Nagayoshi M, Nishihara T, Nakashima K, Iwaki S, Chen KK, Terashita M, et al. Bactericidal Effects of Diode Laser Irradiation on Enterococcus faecalis Using Periapical Lesion Defect Model. Int Sch Res Notices 2011; 2011: 870364.
- Souza LC, Brito PR, de Oliveira JC, Alves FR, Moreira EJ, SampaioFilho HR, et al. Photodynamic therapy with two different photosensitizers as a supplement to instrumentation/irrigation procedures in promoting intracanal reduction of Enterococcus faecalis. J Endod 2010; 36: 292-96.
- Juric IB, Plecko V, Panduric DG, Anic I. The antimicrobial effectiveness of photodynamic therapy used as an addition to the conventional endodontic re-treatment: a clinical study. Photodiagnosis Photodyn Ther 2014; 11: 549-55.
- Trindade AC, De Figueiredo JA, Steier L, Weber JB. Photodynamic therapy in endodontics: a literature review. Photomed Laser Surg 2015; 33: 175-82.
- Bago I, Plecko V, Gabric Panduric D, Schauperl Z, Baraba A, Anic I. Antimicrobial efficacy of a high-power diode laser, photoactivated disinfection, conventional and sonic activated irrigation during root canal treatment. Int Endod J 2013; 46: 339-47.
- Carvalho Edos S, Mello I, Albergaria SJ, Habitante SM, LageMarques JL, Raldi DP. Effect of chemical substances in removing methylene blue after photodynamic therapy in root canal treatment. Photomed Laser Surg 2011; 29: 559-63.
| APA | ÖZKOÇAK İ, Göktürk H, ŞAY COŞKUN U, AYTAÇ BAL F (2018). Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis. , 289 - 295. 10.4274/meandros.08370 |
| Chicago | ÖZKOÇAK İSMAİL,Göktürk Hakan,ŞAY COŞKUN UMUT SAFİYE,AYTAÇ BAL FATMA Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis. (2018): 289 - 295. 10.4274/meandros.08370 |
| MLA | ÖZKOÇAK İSMAİL,Göktürk Hakan,ŞAY COŞKUN UMUT SAFİYE,AYTAÇ BAL FATMA Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis. , 2018, ss.289 - 295. 10.4274/meandros.08370 |
| AMA | ÖZKOÇAK İ,Göktürk H,ŞAY COŞKUN U,AYTAÇ BAL F Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis. . 2018; 289 - 295. 10.4274/meandros.08370 |
| Vancouver | ÖZKOÇAK İ,Göktürk H,ŞAY COŞKUN U,AYTAÇ BAL F Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis. . 2018; 289 - 295. 10.4274/meandros.08370 |
| IEEE | ÖZKOÇAK İ,Göktürk H,ŞAY COŞKUN U,AYTAÇ BAL F "Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis." , ss.289 - 295, 2018. 10.4274/meandros.08370 |
| ISNAD | ÖZKOÇAK, İSMAİL vd. "Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis". (2018), 289-295. https://doi.org/10.4274/meandros.08370 |
| APA | ÖZKOÇAK İ, Göktürk H, ŞAY COŞKUN U, AYTAÇ BAL F (2018). Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis. Meandros Medical And Dental Journal, 19(4), 289 - 295. 10.4274/meandros.08370 |
| Chicago | ÖZKOÇAK İSMAİL,Göktürk Hakan,ŞAY COŞKUN UMUT SAFİYE,AYTAÇ BAL FATMA Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis. Meandros Medical And Dental Journal 19, no.4 (2018): 289 - 295. 10.4274/meandros.08370 |
| MLA | ÖZKOÇAK İSMAİL,Göktürk Hakan,ŞAY COŞKUN UMUT SAFİYE,AYTAÇ BAL FATMA Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis. Meandros Medical And Dental Journal, vol.19, no.4, 2018, ss.289 - 295. 10.4274/meandros.08370 |
| AMA | ÖZKOÇAK İ,Göktürk H,ŞAY COŞKUN U,AYTAÇ BAL F Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis. Meandros Medical And Dental Journal. 2018; 19(4): 289 - 295. 10.4274/meandros.08370 |
| Vancouver | ÖZKOÇAK İ,Göktürk H,ŞAY COŞKUN U,AYTAÇ BAL F Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis. Meandros Medical And Dental Journal. 2018; 19(4): 289 - 295. 10.4274/meandros.08370 |
| IEEE | ÖZKOÇAK İ,Göktürk H,ŞAY COŞKUN U,AYTAÇ BAL F "Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis." Meandros Medical And Dental Journal, 19, ss.289 - 295, 2018. 10.4274/meandros.08370 |
| ISNAD | ÖZKOÇAK, İSMAİL vd. "Antibacterial Efficiency of Different Irrigation Solutions, Lasers and Photodynamic Therapy with Indocyanine Green in Root Canals Infected By Enterococcus Faecalis". Meandros Medical And Dental Journal 19/4 (2018), 289-295. https://doi.org/10.4274/meandros.08370 |
