In this work, the effect of project based learning approach on students’ academic achievement on the topic of “Application of UV/Visible spectroscopic analysis method based on light-matter interaction” in Analytical Chemistry Laboratory lesson. Pre- and post-test quasi experimental design with control group was applied in this research. In the experimental group, the subject was carried out with the project based learning approach and in the control group in accordance with the teaching program. It was observed that there was a significant difference in the students’ academic achievement in favour of the experimental group in which the project based learning approach was used according to the Mann-Whitney U test analysis results of the post-test academic achievement scores (p<.05). Wilcoxon signed rank test results showed that the increase of students’ academic achievement of within both groups. It can be also stated that project based learning approach has a great effect size considering to Cohen’s d value (d=0.88). Content analysis of semistructured interview results demonstrated that the experimental group students find it more memorable to process the subject with an applied approach.