Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment
Yıl: 2018 Cilt: 31 Sayı: 1 Sayfa Aralığı: 11 - 15 Metin Dili: İngilizce İndeks Tarihi: 24-07-2019
Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment
Öz: Objective: The objective of this study is to describe the potatodextrose agar (PDA) with rose-Bengal and chloramphenicol asa new and simple medium (R-PDA chloramphenicol agar) tofacilitate the detection of black yeast, Exophiala dermatitidiscompared to ready-made conventional medium, namely rose-Bengal chloramphenicol agar.Methods: We prepared a new medium by adding chloramphenicoland rose-Bengal to ready-made PDA.Results: This medium proves better growth of the black yeast interms of increased colony size compared to commercial rose-Bengal chloramphenicol agar. The increase in colony size aidsfor distinguishing the slow growing black yeast from all theother filamentous fungi.Conclusions: Compared to traditional rose-Bengal chloramphenicolagar, R-PDA chloramphenicol agar is superior to isolateExophiala dermatitidis among other fast growing filamentousfungi which are present in the environment samples.Klimik Dergisi 2018; 31(1): 11-5.
Anahtar Kelime: Konular:
Patojen Exophiala dermatitidis’i Çevreden İzole Etmek İçin Yeni Bir Besiyeri: “Rose”- Bengal ve Kloramfenikol Eklenmiş Patates Dekstroz Agarı
Öz: Amaç: Bu çalışmanın amacı, kullanıma hazır konvansiyonel bir besiyeri olan “rose”-Bengal kloramfenikol agarıyla karşılaştırıldığında, kara maya olarak adlandırılan Exophiala dermatitidis'in saptanmasını kolaylaştıran yeni ve basit bir besiyeri olarak “rose”-Bengal ve kloramfenikol eklenmiş patates dekstroz agarı (PDA)’nı (R-PDA kloramfenikol agarı) tanıtmaktır. Yöntemler: Hazır PDA’ya kloramfenikol ve “rose”-Bengal boyası ekleyerek yeni bir besiyeri hazırlanmıştır. Bulgular: Kara mayanın, bu besiyerinde, ticari “rose”-Bengal kloramfenikol besiyeriyle karşılaştırıldığında, daha büyük koloniler oluşturduğu kanıtlanmıştır. Kolonilerin daha büyük olması, bu yavaş üreyen kara mayanın, diğer ipliksi mantarlardan ayırt edilmesini kolaylaştırmıştır. Sonuçlar: Yeni bir besiyeri olan R-PDA kloramfenikol agarı, yavaş çoğalan Exophiala dermatitidis’in, çevre örneklerinde bulunan diğer hızlı çoğalan ipliksi mantarların arasında izole edilmesi için, geleneksel “rose”-Bengal kloramfenikol agarından daha üstündür. Klimik Dergisi 2018; 31(1): 11-5.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | JAYARAM M, NAGAO H (2018). Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment. , 11 - 15. |
| Chicago | JAYARAM Mehalene,NAGAO Hideyuki Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment. (2018): 11 - 15. |
| MLA | JAYARAM Mehalene,NAGAO Hideyuki Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment. , 2018, ss.11 - 15. |
| AMA | JAYARAM M,NAGAO H Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment. . 2018; 11 - 15. |
| Vancouver | JAYARAM M,NAGAO H Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment. . 2018; 11 - 15. |
| IEEE | JAYARAM M,NAGAO H "Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment." , ss.11 - 15, 2018. |
| ISNAD | JAYARAM, Mehalene - NAGAO, Hideyuki. "Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment". (2018), 11-15. |
| APA | JAYARAM M, NAGAO H (2018). Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment. Klimik Dergisi, 31(1), 11 - 15. |
| Chicago | JAYARAM Mehalene,NAGAO Hideyuki Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment. Klimik Dergisi 31, no.1 (2018): 11 - 15. |
| MLA | JAYARAM Mehalene,NAGAO Hideyuki Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment. Klimik Dergisi, vol.31, no.1, 2018, ss.11 - 15. |
| AMA | JAYARAM M,NAGAO H Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment. Klimik Dergisi. 2018; 31(1): 11 - 15. |
| Vancouver | JAYARAM M,NAGAO H Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment. Klimik Dergisi. 2018; 31(1): 11 - 15. |
| IEEE | JAYARAM M,NAGAO H "Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment." Klimik Dergisi, 31, ss.11 - 15, 2018. |
| ISNAD | JAYARAM, Mehalene - NAGAO, Hideyuki. "Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment". Klimik Dergisi 31/1 (2018), 11-15. |
