Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması
Yıl: 2019 Cilt: 6 Sayı: 2 Sayfa Aralığı: 74 - 80 Metin Dili: Türkçe DOI: 10.34087/cbusbed.405472 İndeks Tarihi: 06-01-2020
Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması
Öz: Amaç: Kanser, doku ve organları istila eden ve vücudun diğer kısımlarına sıçrayabilen, hücrelerin anormal gelişimidir. Meme kanseri en çok tekrarlanan tanıya sahip ve dünya genelinde kadınlarda kanserle ilişkili ölümlerde en önde gelen sebeplerdendir. Tıbbi bitkiler bazı kanser türlerinde antikarsinojenik özelliklere sahip olabilmektedir. Bu çalışmanın amacı Origanum minutiflorum O. Schwarz & P.H. Davis’in in vitro şartlarda, meme kanseri hücre dizilerinde sitotoksik etkisini araştırmaktır.Gereç ve Yöntem: Bu çalışmada, MDA-MB-231 ve MCF-7 meme kanseri hücre dizileri ve adipoz dokudan izole edilmiş mezenkimal kök hücreleri kullanıldı. Origanum minutiflorum’dan elde edilmiş esansiyel yağa maruz bırakılmış tüm hücre dizilerinde MTT testi aracılığıyla IC50 dozu hesaplandı. Her bir hücre dizisinin IC50 dozunda eNOS, p53, PCNA ve TUNEL için immunositokimya boyaması yapıldı. Sonuçlar Graphpad yazılımı kullanılarak one-way-ANOVA kullanılarak değerlendirildi.Bulgular: Meme kanseri hücrelerinin proliferasyonu oregano yağı tarafından inhibe edildi. Oregano yağı mezenkimal kök hücrelerinde herhangi bir sitotoksik etki göstermedi. eNOS ve p53 ile apoptozisin göstergesi TUNEL’de artış yoluyla oregano yağının kanser hücrelerinde sitotoksik olduğu ortaya çıktı ve PCNA boyamada proliferasyonun azaldığı tespit edildi.Sonuç: Bizim verilerimize göre oregano yağı MDA-MB-231 ve MCF-7 meme kanseri hücrelerinde sitotoksik bir etkiye sahiptir ve diğer biyolojik aktiviteleri için aktif komponentlerini tanımlayacak testlere ihtiyaç vardır.
Anahtar Kelime: -
Öz: Objective: Cancer is defined by a abnormal growth of cells, that invade tissues and organs and spread to other parts of the body. Breast cancer is one of the most repeatedly diagnosed cancers and the foremost cause of death among women worldwide. Medicinal plants can have an anticarcinogenic effect in some cancer types. Our aim is to search the cytotoxic effect of Origanum minutiflorum O. Schwarz & P.H. Davis on breast cancer cells in vitro conditions. Methods: In this study, MDA-MB-231 and MCF-7 breast cancer cell lines and adipose tissue derived mesenchymal stem cells were used. Essential oil from Origanum minutiflorum was exposed to the all cells and IC50 dose was calculated by MTT assay. We have examined immunocytochemical staining of eNOS, p53, PCNA and TUNEL at IC50 dose for each cells. The results were evaluated using one-way-ANOVA by Graphpad software. Results: The proliferation of breast cancer cells were inhibited by oregano oil. Oregano oil did not show any cytotoxic effect in mesenchymal stem cells. It was determined that cytotoxic effect of oregano oil on cancer cells was occurred through increase of eNOS and p53 staining for survival and the TUNEL labeling for apoptosis, and decreased of PCNA staining for proliferation. Conclusion: According to our data, oregano oil has cytotoxic effect on MDA-MB-231 and MCF-7 breast cancer cell lines, and it needs to be analysis to detect active components for other biological activities.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Perumalsamya, H, Sankarapandian, K, Kandaswamy, N, Balusamy, S.R, Periyathambi, D, Raveendiran, N, Cellular effect of styrene substituted biscoumarin caused cellular apoptosis and cell cycle arrest in human breast cancer cells. Int J Biochem Cell B, 2017, 92:104–14.
- Costa Araldi, I.C, Bordin, F.P.R, Cadoná, F.C, Barbisan, F, Azzolin, V.F, Teixeira, C.F, Baumhardt, T, Cruz, I.B.M, Duarte, M.M.M.F, Bauermann, L.F, The in vitro radiosensitizer potential of resveratrol on MCF-7 breast cancer cells. Chem-Biol Interact, 2018, DOI: 10.1016/j.cbi.2018.01.013.
- Saueressig, S, Tessmann, J, Mastelari, R, Pereira da Silva, L, Buss, J, Segatto, N.V, Begnini, K.R, Pacheco, B, Pereira de Pereira, C.M, Collares, T, Seixas, F.K, Synergistic effect of pyrazoles derivatives and doxorubicin in claudin-low breast cancer subtype. Biomed Pharmacother, 2018, 98: 390–8.
- Park, E.J, Lee, D, Baek, S.E, Kim, K.H, Kang, K.S, Jang, T.S, Lee, H.L, Song, J.H, Yoo, J.E, Cytotoxic effect of sanguiin H-6 on MCF-7 and MDA-MB-231 human breast carcinoma cells. Bioorg Med Chem Lett, 2017, 27: 4389–92.
- Subash-Babu, P, Li, D.K, Alshatwi, A.A, In vitro cytotoxic potential of friedelin in human MCF-7 breast cancer cell: Regulate early expression of Cdkn2a and pRb1, neutralize mdm2-p53 amalgamation and functional stabilization of p53. Exp Toxicol Pathol, 2017, 69: 630–6.
- Zarei, O, Yaghoobi, M.M, Cytotoxic effects of Fritillaria imperialis L. extracts on human liver cancer cells, breast cancer cells and fibroblast-like cells. Biomed Pharmacother, 2017, 94: 598–604.
- Kumari, S, Badana, A.K, Mohan, G.M, Naik, G.S, Malla, R.R, Synergistic effects of coralyne and paclitaxel on cell migration and proliferation of breast cancer cells lines. Biomed Pharmacother, 2017, 91: 436-45.
- Maryam, R, Faegheh, S, Majid, A.S, Kazem, N.K, Effect of quercetin on secretion and gene expression of leptin in breast cancer. J Tradit Chin Med, 2017, 37(3): 321-5.
- Wang, G, Li, J, Zhang, L, Huang, S, Zhao, X, Zhao, X, Celecoxib induced apoptosis against different breast cancer cell lines by down-regulated NF-κB pathway. Biochem Biophys Res Commun, 2017, pii: S0006-291X(17)31279-2. doi:10.1016/j.bbrc.2017.06.148.
- Wang, X, Liu, X, Li, Y, Wang, P, Feng, X, Liu, Q, Yan, F, Zheng, H, Sensitivity to antitubulin chemotherapeutics is potentiated by a photoactivable nanoliposome. Biomaterials, 2017, 141:50-62. doi: 10.1016/j.biomaterials.2017.06.034.
- de Oliveira Mauro, M, Matuo, R, de David, N, Strapasson, R.L.B, Oliveira, R.J, Stefanello, M.É.A, Kassuya, C.A.L, de Cepa Matos, M.F, Faria, F.J.C, Costa, D.S, Actions of sesquiterpene lactones isolated from Moquiniastrum polymorphum subsp. Floccosum in MCF7 cell line and their potentiating action on doxorubicin. BMC Pharmacol Toxicol, 2017, 18(1):53. doi: 10.1186/s40360-017-0156-3.
- Li, J, Yao, Q.Y, Xue, J.S, Wang, L.J, Yuan, Y, Tian, X.Y, Su, H, Wang, S.Y, Chen, W.J, Lu, W, Zhou, T.Y, Dopamine D2 receptor antagonist sulpiride enhances dexamethasone responses in the treatment of drug-resistant and metastatic breast cancer. Acta Pharmacol Sin, 2017, doi: 10.1038/aps.2017.24.
- Yao, M, Yuan, B, Wang, X, Sato, A, Sakuma, K, Kaneko, K, Komuro, H, Okazaki, A, Hayashi, H, Toyoda, H, Pei, X, Hu, X, Hirano, T, Takagi, N, Synergistic cytotoxic effects of arsenite and tetrandrine in human breast cancer cell line MCF-7. Int J Oncol, 2017, doi: 10.3892/ijo.2017.4052.
- Liu, Y, Wang, R, Zhang, L, Li, J, Lou, K, Shi, B, The lipid metabolism gene FTO influences breast cancer cell energy metabolism via the PI3K/AKT signaling pathway. Oncol Lett, 2017, 13(6):4685-4690. doi: 10.3892/ol.2017.6038.
- Chen, S, Meng, T, Zheng, X, Cai, J, Zhang, W, You, H, Xing, J, Dong, Y, Contribution of nucleophosmin overexpression to multidrug resistance in breast carcinoma. J Drug Target, 2017, 1-9. doi: 10.1080/1061186X.2017.1332066.
- Chun, S.Y, Kim, S, Nam, K.S, The inhibitory effects of deep-sea water on doxorubicin induced epithelial-mesenchymal transition. Oncol Rep, 2017, doi: 10.3892/or.2017.5726.
- Panjehpour, M, Castro, M, Klotz, K.N, Human breast cancer cell line MDA-MB-231 expresses endogenous A2B adenosine receptors mediating a Ca2+ signal. Br J Pharmacol, 2005, 145(2):211-8.
- Elamin, M.H, Elmahi, A.B, Daghestani, M.H, Al-Olayan, E.M, Al-Ajmi, R.A, Alkhuriji, A.F, Hamed, S.S, Elkhadragy, M.F, Synergistic Anti-Breast-Cancer Effects of Combined Treatment With Oleuropein and Doxorubicin In Vivo. Altern Ther Health Med, 2017, pii: AT5644.
- Lokerse, W.J.M, Bolkestein, M, Dalm, S.U, Eggermont, A.M.M, de Jong, M, Grüll, H, Koning, G.A, Comparing the therapeutic potential of thermosensitive liposomes and hyperthermia in two distinct subtypes of breast cancer. J Control Release, 2017, 258:34-42. doi: 10.1016/j.jconrel.2017.05.005.
- Brito, L.C, Berenger, A.L.R, Figueiredo, M.R, An overview of anticancer activity of Garcinia and Hypericum. Food Chem Toxicol, 2017, 109: 847-62.
- Hajlaoui, H, Mighri, H, Aouni, M, Gharsallah, N, Kadri, A, Chemical composition and in vitro evaluation of antioxidant, antimicrobial, cytotoxicity and anti- acetylcholinesterase properties of Tunisian Origanum majorana L. essential oil. Microb Pathog, 2016, 95: 86-94.
- Bouyahya, A, Bakri, Y, Et-Touys, A, Assemian, I.C.C, Abrini, J, Dakka, N, In vitro antiproliferative activity of selected medicinal plants from the North-West of Morocco on several cancer cell lines. Eur J Integr Med, 2018, 18: 23–9.
- Bostancıoğlu, R.B, Kurkcuoğlu, M, Başer, K.H.C, Koparal, A.T, Assessment of anti-angiogenic and anti-tumoral potentials of Origanum onites L. essential oil. Food Chem Toxicol, 2012, 50: 2002–8.
- Han, X, Parker, T.L, Anti-inflammatory, tissue remodeling, immunomodulatory, and anticancer activities of oregano (Origanum vulgare) essential oil in a human skin disease model. Biochim Open, 2017, 4: 73-7.
- Abdel-Massih, R.M, Fares, R, Bazzi, S, El-Chami, N, Baydoun, E, The apoptotic and anti-proliferative activity of Origanum majorana extracts on human leukemic cell line. Leuk Res, 2010, 34: 1052–6.
- Al-Kalaldeh, J.Z, Abu-Dahab, R, Afifi, F.U, Volatile oil composition and antiproliferative activity of Laurus nobilis, Origanum syriacum, Origanum vulgare, and Salvia triloba against human breast adenocarcinoma cells. Nutr Res, 2010, 30: 271–8.
- Baydar, H, Yayla kekiği (origanum minutiflorum o. schwarz et. p. h. davis)'nde farklı toplama zamanlarının uçucu yağ içeriği ve uçucu yağ bileşenleri üzerine etkisi. Mediterr Agric Sci, 2005, 18(2): 175-8.
- Tuğlu, M.İ, Özdal-Kurt, F, Koca, H, Sarac, A, Barut, T, Kazanç, A, The Contribution of Differentiated Bone Marrow Stromal Stem CellLoaded Biomaterial to Treatment in Critical Size Defect Model in Rats. Kafkas Univ Vet Fak Derg, 2010, 16 (5): 783-92.
- Uluer, E.T, Aydemir, I, Inan, S, Ozbilgin, K, Vatansever, H.S, Effects of 5-fluorouracil and gemcitabine on a breast cancer cell line (MCF-7) via the JAK/STAT pathway. Acta Histochem, 2012, 114(7): 641-6.
- Deliloglu, G.I, Tuglu, I, Vatansever, H.S, Ozdal-Kurt, F, Ekren, H, Taylan, M, Sen, B.H, The effect of osteogenic medium on the adhesion of rat bone marrow stromal cell to the hydroxyapatite. Saudi Med J, 2006, 27(3): 305-11.
- Kurt, F.Ö, Tuğlu, I, Vatansever, H.S, Tong, S, Şen, B.H, Deliloğlu-Gürhan, S.I, The effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblasts. Biotech Histochem, 2016, 91(6): 412-22.
- Karabuga, I, Akbay, K, Turna, B, Vatansever, H.S, Altay, B, Güzel, E, Turkoz-Uluer, E, Ustun, G, Ekren, F, Nazli, O, Muftuoglu, S, Apaydin, E, Effect of lisinopril on renal tissue damage in unilateral ureteral obstruction in rats. Urol Res, 2012, 40(1): 27-34.
- Gürpınar, T, Ekerbiçer, N, Uysal, N, Barut, T, Tarakçı, F, Tuglu, M.I, The effects of the melatonin treatment on the oxidative stress and apoptosis in diabetic eye and brain. Scientific World Journal, 2012, 2012:498489.
- Ozbilgin, K, Karaca, F, Turan, A, Köse, C, Vatansever, S, Ozcakir, T, The higher heparin-binding epidermal growth factor (HB-EGF) in missed abortion. Taiwan J Obstet Gynecol, 2015, 54(1): 13-8.
- Hack, C.C, Voiß, P, Lange, S, Paul, A.E, Conrad, S, Dobos, G.J, Beckmann, M.W, Kümmel, S, Local and Systemic Therapies for Breast Cancer Patients: Reducing Short-term Symptoms with the Methods of Integrative Medicine. Geburtshilfe Frauenheilkd, 2015, 75(7): 675-82.
- Kim, W, Lee, W.B, Lee, J.W, Min, B.I, Baek, S.K, Lee, H.S, Cho, S.H, Traditional herbal medicine as adjunctive therapy for breast cancer: A systematic review. Complement Ther Med, 2015, 23(4): 626-32.
- Zheng, J, Zhou, Y, Li, Y, Xu, D.P, Li, S, Li, H.B, Spices for Prevention and Treatment of Cancers. Nutrients, 2016, 8(8): E495.
- Tariq, A, Sadia, S, Pan, K, Ullah, I, Mussarat, S, Sun, F, Abiodun, O.O, Batbaatar, A, Li, Z, Song, D, Xiong, Q, Ullah, R, Khan, S, Basnet, B.B, Kumar, B, Islam, R, Adnan, M, A systematic review on ethnomedicines of anti-cancer plants. Phytother Res, 2017, 31(2): 202-64.
- Moore, J, Yousef, M, Tsiani, E, Anticancer Effects of Rosemary (Rosmarinus officinalis L.) Extract and Rosemary Extract Polyphenols. Nutrients, 2016, 8(11): E731.
- Santos, P.A, Avanço, G.B, Nerilo, S.B, Marcelino, R.I, Janeiro, V, Valadares, M.C, Machinski, M, Assessment of Cytotoxic Activity of Rosemary (Rosmarinus officinalis L.), Turmeric (Curcuma longa L.), and Ginger (Zingiber officinale R.) Essential Oils in Cervical Cancer Cells (HeLa). Scientific World Journal, 2016, 2016:9273078.
- Zaker, A, Asili, J, Abrishamchi, P, Tayarani-Najaran, Z, Mousavi, S.H, Cytotoxic and apoptotic effects of root extract and tanshinones isolated from Perovskiaabrotanoides Kar. Iran J Basic Med Sci, 2017, 20(12): 1377-84.
- Rolim, T.L, Meireles, D.R.P, Batista, T.M, de Sousa, T.K.G, Mangueira, V.M, de Abrantes, R.A, Pita, J.C.L.R, Xavier, A.L, Costa, V.C.O, Batista, L.M, da Silva, M.S, Sobral, M.V, Toxicity and antitumor potential of Mesosphaerum sidifolium (Lamiaceae) oil and fenchone, its major component. BMC Complement Altern Med, 2017, 17(1):347.
- Fekrazad, R, Afzali, M, Pasban-Aliabadi, H, Esmaeili-Mahani, S, Aminizadeh, M, Mostafavi, A, Cytotoxic Effect of Thymus caramanicus Jalas on Human Oral Epidermoid Carcinoma KB Cells. Braz Dent J, 2017, 28(1): 72-7.
- Oke, F, Aslim, B, Biological potentials and cytotoxicity of various extracts from endemic Origanum minutiflorum O. Schwarz & P.H. Davis. Food Chem Toxicol, 2010, 48(6): 1728-33.
- Dadalioglu, I, Evrendilek, G.A, Chemical compositions and antibacterial effects of essential oils of Turkish oregano (Origanum minutiflorum), bay laurel (Laurus nobilis), Spanish lavender (Lavandula stoechas L.), and fennel (Foeniculum vulgare) on common foodborne pathogens. J Agric Food Chem, 2004, 52(26): 8255-60.
- Dorman, H.J, Bachmayer, O, Kosar, M, Hiltunen, R, Antioxidant properties of aqueous extracts from selected lamiaceae species grown in Turkey. J Agric Food Chem, 2004, 52(4): 762-70.
- Cetin, H, Yanikoglu, A, A study of the larvicidal activity of Origanum (Labiatae) species from southwest Turkey. J Vector Ecol, 2006, 31(1): 118-22.
- Cetin, H, Cilek, J.E, Aydin, L, Yanikoglu, A, Acaricidal effects of the essential oil of Origanum minutiflorum (Lamiaceae) against Rhipicephalus turanicus (Acari: Ixodidae). Vet Parasitol, 2009, 160 (3-4):359-61.
- Süntar, I, Akkol, E.K, Keleş, H, Oktem, A, Başer, K.H, Yeşilada, E, A novel wound healing ointment: a formulation of Hypericum perforatum oil and sage and oregano essential oils based on traditional Turkish knowledge. J Ethnopharmacol, 2011, 134(1): 89-96.
- Akdemir Evrendilek, G, Empirical prediction and validation of antibacterial inhibitory effects of various plant essential oils on common pathogenic bacteria. Int J Food Microbiol, 2015, 202: 35-41.
| APA | OZKAN O, ÖZBEY C, AYDEMİR I, Sari I, DÖNMEZ ARAT G, AYDIN F, Savran A, TUĞLU M (2019). Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması. , 74 - 80. 10.34087/cbusbed.405472 |
| Chicago | OZKAN OKTAY,ÖZBEY Caner,AYDEMİR IŞIL,Sari Ismail,DÖNMEZ ARAT GONCA,AYDIN FATMA ESIN,Savran Ahmet,TUĞLU Mehmet İbrahim Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması. (2019): 74 - 80. 10.34087/cbusbed.405472 |
| MLA | OZKAN OKTAY,ÖZBEY Caner,AYDEMİR IŞIL,Sari Ismail,DÖNMEZ ARAT GONCA,AYDIN FATMA ESIN,Savran Ahmet,TUĞLU Mehmet İbrahim Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması. , 2019, ss.74 - 80. 10.34087/cbusbed.405472 |
| AMA | OZKAN O,ÖZBEY C,AYDEMİR I,Sari I,DÖNMEZ ARAT G,AYDIN F,Savran A,TUĞLU M Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması. . 2019; 74 - 80. 10.34087/cbusbed.405472 |
| Vancouver | OZKAN O,ÖZBEY C,AYDEMİR I,Sari I,DÖNMEZ ARAT G,AYDIN F,Savran A,TUĞLU M Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması. . 2019; 74 - 80. 10.34087/cbusbed.405472 |
| IEEE | OZKAN O,ÖZBEY C,AYDEMİR I,Sari I,DÖNMEZ ARAT G,AYDIN F,Savran A,TUĞLU M "Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması." , ss.74 - 80, 2019. 10.34087/cbusbed.405472 |
| ISNAD | OZKAN, OKTAY vd. "Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması". (2019), 74-80. https://doi.org/10.34087/cbusbed.405472 |
| APA | OZKAN O, ÖZBEY C, AYDEMİR I, Sari I, DÖNMEZ ARAT G, AYDIN F, Savran A, TUĞLU M (2019). Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 6(2), 74 - 80. 10.34087/cbusbed.405472 |
| Chicago | OZKAN OKTAY,ÖZBEY Caner,AYDEMİR IŞIL,Sari Ismail,DÖNMEZ ARAT GONCA,AYDIN FATMA ESIN,Savran Ahmet,TUĞLU Mehmet İbrahim Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 6, no.2 (2019): 74 - 80. 10.34087/cbusbed.405472 |
| MLA | OZKAN OKTAY,ÖZBEY Caner,AYDEMİR IŞIL,Sari Ismail,DÖNMEZ ARAT GONCA,AYDIN FATMA ESIN,Savran Ahmet,TUĞLU Mehmet İbrahim Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, vol.6, no.2, 2019, ss.74 - 80. 10.34087/cbusbed.405472 |
| AMA | OZKAN O,ÖZBEY C,AYDEMİR I,Sari I,DÖNMEZ ARAT G,AYDIN F,Savran A,TUĞLU M Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi. 2019; 6(2): 74 - 80. 10.34087/cbusbed.405472 |
| Vancouver | OZKAN O,ÖZBEY C,AYDEMİR I,Sari I,DÖNMEZ ARAT G,AYDIN F,Savran A,TUĞLU M Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi. 2019; 6(2): 74 - 80. 10.34087/cbusbed.405472 |
| IEEE | OZKAN O,ÖZBEY C,AYDEMİR I,Sari I,DÖNMEZ ARAT G,AYDIN F,Savran A,TUĞLU M "Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması." Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 6, ss.74 - 80, 2019. 10.34087/cbusbed.405472 |
| ISNAD | OZKAN, OKTAY vd. "Kanser hücreleri üzerine Origanum Minutiflorum’un Sitotoksik Etkisinin Araştırılması". Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 6/2 (2019), 74-80. https://doi.org/10.34087/cbusbed.405472 |
