Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease

Yıl: 2018 Cilt: 18 Sayı: 2 Sayfa Aralığı: 310 - 320 Metin Dili: İngilizce DOI: 10.26650/electrica.2018.12050 İndeks Tarihi: 04-12-2019

Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease

Öz:
Alzheimer's disease (AD) is a neurodegenerative disease and is identified by the detection of amyloid-plaques and neurofibrillary tangles in the brain.Amyloid precursor protein gene, presenilin 1 (PSEN1) gene, and presenilin 2 (PSEN2) gene are responsible for this disease. PSEN2 and amyloid precursorprotein (APP) gene mutations are a much rarer cause of familial AD patients. This study aims to clone the PSEN2 gene and create vectors with differentmutations by directed mutagenesis. As a result of the experiments, the PSEN2 cDNA was cloned between the BamHI and KpnI cut-off points of thepBluescript II sk (+) vector. PSEN1 and PSEN2 homologs have a role in cell destiny decision and AD progress. We studied some of the PSEN2 mutations(Ala252Thr and Pro334Arg) and provided expression analysis in eukaryotic cell cultures. Amyloid β-protein (Aβ), which is produced by endoproteolyticcleavage of the APP, is considered to play a role in AD. While nominal concentration of Aβ40 is 10 times of Aβ42, the last peptide is firmly linked to ADpathogenesis. Amyloid β-protein is generated by the γ-secretase cleavage of APP onset and the progression of AD, and it is the primary ingredient of thesenile plaques. The Aβ42 dodecamer plays a central role in AD. In future studies, it will be determined if there is an increase in Aβ42 protein levels, andthe effect on this early onset AD can be identified.
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Konular: Mühendislik, Elektrik ve Elektronik
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Öztan G, YOKEŞ B, İŞSEVER H (2018). Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease. , 310 - 320. 10.26650/electrica.2018.12050
Chicago Öztan Gözde,YOKEŞ Baki,İŞSEVER Halim Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease. (2018): 310 - 320. 10.26650/electrica.2018.12050
MLA Öztan Gözde,YOKEŞ Baki,İŞSEVER Halim Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease. , 2018, ss.310 - 320. 10.26650/electrica.2018.12050
AMA Öztan G,YOKEŞ B,İŞSEVER H Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease. . 2018; 310 - 320. 10.26650/electrica.2018.12050
Vancouver Öztan G,YOKEŞ B,İŞSEVER H Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease. . 2018; 310 - 320. 10.26650/electrica.2018.12050
IEEE Öztan G,YOKEŞ B,İŞSEVER H "Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease." , ss.310 - 320, 2018. 10.26650/electrica.2018.12050
ISNAD Öztan, Gözde vd. "Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease". (2018), 310-320. https://doi.org/10.26650/electrica.2018.12050
APA Öztan G, YOKEŞ B, İŞSEVER H (2018). Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease. Electrica, 18(2), 310 - 320. 10.26650/electrica.2018.12050
Chicago Öztan Gözde,YOKEŞ Baki,İŞSEVER Halim Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease. Electrica 18, no.2 (2018): 310 - 320. 10.26650/electrica.2018.12050
MLA Öztan Gözde,YOKEŞ Baki,İŞSEVER Halim Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease. Electrica, vol.18, no.2, 2018, ss.310 - 320. 10.26650/electrica.2018.12050
AMA Öztan G,YOKEŞ B,İŞSEVER H Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease. Electrica. 2018; 18(2): 310 - 320. 10.26650/electrica.2018.12050
Vancouver Öztan G,YOKEŞ B,İŞSEVER H Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease. Electrica. 2018; 18(2): 310 - 320. 10.26650/electrica.2018.12050
IEEE Öztan G,YOKEŞ B,İŞSEVER H "Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease." Electrica, 18, ss.310 - 320, 2018. 10.26650/electrica.2018.12050
ISNAD Öztan, Gözde vd. "Cloning of Presenilin 2 cDNA and Construction of Vectors Carrying Effective Mutations in the Pathogenesis of Familial Alzheimer's Disease". Electrica 18/2 (2018), 310-320. https://doi.org/10.26650/electrica.2018.12050