The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III

KARADAŞ, Erkan; ÖZKUL, Ayhan; DEMİREL, Hasan Hüseyin; Bozkurt, Mehmet Fatih; KELEŞ, Hikmet; ULUCAN, Aykut; YÜKSEL, HAYATİ

The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III

Hikmet KELEŞ, (Afyon Kocatepe University)

Mehmet Fatih BOZKURT, (Afyon Kocatepe University)

Aykut ULUCAN, (Bingöl University)

Hasan HÜSEYİN DEMİREL, (Afyon Kocatepe University)

Hayati YÜKSEL, (Bingöl University)

Erkan KARADAŞ, (Afyon Kocatepe University)

Ayhan ÖZKUL (Ankara University)

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2018 Cilt: 65 Sayı: 4 Sayfa Aralığı: 341 - 348 Metin Dili: İngilizce İndeks Tarihi: 10-12-2019

The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III

Öz:

Changes in uroplakin secretion in the urothelium leads a gradual ability lost in neoplastic conditions. Urothelium is one of the target regions where nitric oxide can damage. Therefore, in this study, the presence of iNOS and UPIII were investigated in bovine enzootic hematuria cases. The results were supported by comparing them with those achieved by critical markers for malignancy of tumor such as caspase 3, Bax, p53, PCNA, and VEGF. The presence of 8-OHDG, a good marker for oxidative DNA damage, was also investigated. For this purpose, urinary bladders with enzootic hematuria were collected from the slaughterhouses. In histopathological examinations, neoplastic, pre-neoplastic, and inflammatory lesions were observed in these urinary bladders. In immunohistochemical examinations, iNOS reacts as negative in the UPIII positive healthy urothelium which preserves the structure of AUM. On the other hand positive reaction has been observed in AUM modified and UPIII negative urothelium. In these cases, as an indicator of oxidative damage, 8-OHdG positivity was observed in the UPIII negative and iNOS positive areas. Additionally, increased cell proliferation, decreased apoptosis as well as p53 disorganization were clearly observed in most of the cases. Thus, the active role of nitric oxide, as a free radical derivative, has been shown in the enzootic hematuria cases of cattle.

Anahtar Kelime:

Konular: Veterinerlik

Enzootik hematürili sığırların idrar kesesinde indüklenebilir nitrik oksit sentaz’ın rolü ve uroplakin III ile mücadelesi

Öz:

Uroplakin sekresyonundaki değişiklikler, neoplazi varlığında ürotelyumda aşamalı bir fonksiyon kaybına neden olur. Ürotelyum nitrik oksit hasarına maruz kalabilen hedef bölgelerden biridir. Bu nedenle, bu çalışmada, enzootik hematüri vakalarında iNOS ve UPIII varlığı araştırıldı. Sonuçlar, apoptozis ve tümör gelişimi için kritik göstergeler olan caspase 3, Bax, p53, PCNA ve VEGF ile karşılaştırılarak desteklendi. Oksidatif DNA hasarı için iyi bir belirteç olan 8-OHdG varlığı da irdelendi. Bu amaçla, mezbahadan enzootik hematüri şüpheli idrar keseleri toplandı. Bu idrar keselerinin histopatolojik incelemelerinde, neoplastik, pre-neoplastik ve yangısal lezyonlar gözlendi. İmmunohistokimyasal incelemelerde, UPIII pozitif yani AUM yapısı korunan sağlıklı ürotelyumda iNOS negatif reaksiyon izlenirken, AUM yapısı modifiye UPIII negatif ürotelyumda iNOS pozitif reaksiyon görüldü. Bu vakalarda, UPIII negatif ve iNOS pozitif alanlarda oksidatif stresin bir göstergesi olarak, 8-OHdG pozitifliği de gözlendi. Ek olarak, hücre proliferasyonunda artma, apoptoziste azalma ve düzensiz p53 varlığı çoğu vakada gözlendi. Böylece, sığırların enzootik hematüri vakalarında bir serbest radikal türevi olan nitrik oksitin aktif rolü ortaya kondu.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KELEŞ H, Bozkurt M, ULUCAN A, DEMİREL H, YÜKSEL H, KARADAŞ E, ÖZKUL A (2018). The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III. , 341 - 348.
Chicago	KELEŞ Hikmet,Bozkurt Mehmet Fatih,ULUCAN Aykut,DEMİREL Hasan Hüseyin,YÜKSEL HAYATİ,KARADAŞ Erkan,ÖZKUL Ayhan The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III. (2018): 341 - 348.
MLA	KELEŞ Hikmet,Bozkurt Mehmet Fatih,ULUCAN Aykut,DEMİREL Hasan Hüseyin,YÜKSEL HAYATİ,KARADAŞ Erkan,ÖZKUL Ayhan The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III. , 2018, ss.341 - 348.
AMA	KELEŞ H,Bozkurt M,ULUCAN A,DEMİREL H,YÜKSEL H,KARADAŞ E,ÖZKUL A The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III. . 2018; 341 - 348.
Vancouver	KELEŞ H,Bozkurt M,ULUCAN A,DEMİREL H,YÜKSEL H,KARADAŞ E,ÖZKUL A The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III. . 2018; 341 - 348.
IEEE	KELEŞ H,Bozkurt M,ULUCAN A,DEMİREL H,YÜKSEL H,KARADAŞ E,ÖZKUL A "The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III." , ss.341 - 348, 2018.
ISNAD	KELEŞ, Hikmet vd. "The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III". (2018), 341-348.

APA	KELEŞ H, Bozkurt M, ULUCAN A, DEMİREL H, YÜKSEL H, KARADAŞ E, ÖZKUL A (2018). The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 65(4), 341 - 348.
Chicago	KELEŞ Hikmet,Bozkurt Mehmet Fatih,ULUCAN Aykut,DEMİREL Hasan Hüseyin,YÜKSEL HAYATİ,KARADAŞ Erkan,ÖZKUL Ayhan The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III. Ankara Üniversitesi Veteriner Fakültesi Dergisi 65, no.4 (2018): 341 - 348.
MLA	KELEŞ Hikmet,Bozkurt Mehmet Fatih,ULUCAN Aykut,DEMİREL Hasan Hüseyin,YÜKSEL HAYATİ,KARADAŞ Erkan,ÖZKUL Ayhan The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.65, no.4, 2018, ss.341 - 348.
AMA	KELEŞ H,Bozkurt M,ULUCAN A,DEMİREL H,YÜKSEL H,KARADAŞ E,ÖZKUL A The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2018; 65(4): 341 - 348.
Vancouver	KELEŞ H,Bozkurt M,ULUCAN A,DEMİREL H,YÜKSEL H,KARADAŞ E,ÖZKUL A The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2018; 65(4): 341 - 348.
IEEE	KELEŞ H,Bozkurt M,ULUCAN A,DEMİREL H,YÜKSEL H,KARADAŞ E,ÖZKUL A "The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 65, ss.341 - 348, 2018.
ISNAD	KELEŞ, Hikmet vd. "The role of inducible nitric oxide synthase in urinary bladders of cattle with enzootic hematuria and its struggle with uroplakin III". Ankara Üniversitesi Veteriner Fakültesi Dergisi 65/4 (2018), 341-348.