Our age’s culture is culture of innovation. Innovation is the most effective way for enterprises to gain competitive advantage in a rapidly changing and developing World. Innovation is the most effective way for enterprises to gain competitive advantage in a rapidly changing and developing World. Innovation can be described as a newness in all products and processes. Innovation is to be different. Innovation requires to see/consider the world, events and cases differently. Recognizing opportunities and transforming threats to opportunity can be achieved through different perspectives. In this context, the main point that needs to be emphasized in terms of innovation is to create a culture that will support the innovation of all employees in the institution. The culture of innovation is the culture where innovative ideas are produced, valued and supported. In order to create and spread innovation culture in enterprises that should be adopted and supported particularly by top managers. Innovation requiring a different culture and perspective should have a transformational leadership that creates a broad vision and shares this vision, supports creativity with intellectual incentive, motivates employees with inspirational motivation and achieves the necessary innovation. Transformational leadership, as the driving force of social development, is of particular importance for innovation. The aim of this study is to examine the influence of transformational leadership on innovation and innovation culture in large-scale enterprises operating in Manisa Organized Industrial Zone (one of Turkey's top 500 major industrial corporations). Also, is to out the level of transformational leadership characteristics of the managers and the level of innovation culture. As a result of the analysis, it was seen that transformational leadership has a positive and significant effect on innovation level and innovation culture.