Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions
Yıl: 2019 Cilt: 29 Sayı: 1 Sayfa Aralığı: 21 - 27 Metin Dili: İngilizce DOI: 10.5222/terh.2019.26918 İndeks Tarihi: 27-01-2020
Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions
Öz: Objective: Circulating tumor cells have an important role in the pathogenesis of metastasis. Metastasis occursthrough few steps including arrival of circulating tumor cells to distant tissue and organs, their adherence to thetarget tissue, and then formation of a new tumor. To understand the mechanism of this process it is necessary toinvestigate the interaction of cancer cells with other molecules and cells of the target tissue, and most importantlyinteraction with lipids forming the cellular membrane.Methods: To better understand the process of cancer cell adhesion onto lipid membranes and the ionic interactionsthat are involved in cell adherence, surfaces functionalized with tethered bilayer lipid membrane (tBLM) were utilized in this work as an experimental platform. Either lipid surfaces functionalized with cationic POEPC: PC or anionic POPS: PC fwere examined to observe the ionic interaction of charged phospholipid membrane and MDA-MB231 breast cancer cells.Results: Adhesions of MDA-MB-231 breast cancer cells and NIH-3T3 mouse fibroblast cells to positively chargedPOEPC: PC lipid surfaces,and their dissemination was observed during examinations using Surface PlasmonResonance (SPR) method. The results were further confirmed with cell viability and proliferation studies that showscationic POEPC: PC lipid surfaces were able to facilitate and increase the cell adhesion.Conclusion: These results reveal the cationic phospholipid structures favour the enhanced cancer cell adhesion.
Anahtar Kelime: Konular:
Meme Kanseri Hücrelerinin Fosfolipit Hücre Zarı ile Etkileşimlerinin İncelenmesi
Öz: Amaç: Dolaşımdaki kanser hücrelerinin metastaz oluşmasındaki rolleri önemlidir. Metastaz kanser hücrelerinin diğer organ ve dokulara ulaşması, ve sonrasında hedef dokuya tutunması sonucunda yeni bir tümör oluşumunun başlaması ile gerçekleşir. Bu mekanizmanın çözümlenebilmesi için kanser hücrelerinin hedef dokudaki diğer moleküllerle, hücrelerle ve en önemlisi hücre zarını oluşturan lipitlerle etkileşiminin incelenmesi gerekmektedir. Yöntem: Bu çalışmada, kanser hücrelerinin fosfolipit hücre zarına tutunma sürecini ve hücre tutunmasına etki eden iyonik etkileşimleri daha iyi anlayabilmek için yüzeye tutturulmuş katmanlı lipit membranlar (tBLM) ile fonksiyonlandırılmış yüzeyler deneysel platform olarak kullanılmıştır. Katyonik POEPC: PC veya anyonik POPS: PC ile fonksiyonlanmış lipit yüzeyler, fosfolipit hücre zarı ve MDA-MB-231 meme kanseri hücreleri arasındaki iyonik etkileşimi gözlemlemek için incelendi. Bulgular: Yüzey Plazmonu Rezonansı (SPR) ile yapılan incelemelerde MDA-MB-231 meme kanseri hücrelerinin ve NIH-3T3 fare fibroblast hücrelerinin pozitif yüklü POEPC: PC lipit yüzeylere tutunduğu ve yayıldığı gözlemlendi. Bu sonuçlar ayrıca hücre canlılığı ve hücre büyümesi analizleri ile doğrulanarak katyonik POEPC: PC lipit yüzeylerinin hücre tutunması prosesini hızlandırdığı ve artışa neden olur yönde etkilediği gözlemlendi. Sonuç: Elde edilen sonuçlar katyonik fosfolipit yapının fazla olduğu hücre zarının kanser hücrelerinin tutunmasını kolaylaştırdığını kanıtlamaktadır.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ARSLAN YILDIZ A (2019). Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions. , 21 - 27. 10.5222/terh.2019.26918 |
| Chicago | ARSLAN YILDIZ AHU Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions. (2019): 21 - 27. 10.5222/terh.2019.26918 |
| MLA | ARSLAN YILDIZ AHU Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions. , 2019, ss.21 - 27. 10.5222/terh.2019.26918 |
| AMA | ARSLAN YILDIZ A Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions. . 2019; 21 - 27. 10.5222/terh.2019.26918 |
| Vancouver | ARSLAN YILDIZ A Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions. . 2019; 21 - 27. 10.5222/terh.2019.26918 |
| IEEE | ARSLAN YILDIZ A "Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions." , ss.21 - 27, 2019. 10.5222/terh.2019.26918 |
| ISNAD | ARSLAN YILDIZ, AHU. "Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions". (2019), 21-27. https://doi.org/10.5222/terh.2019.26918 |
| APA | ARSLAN YILDIZ A (2019). Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions. İzmir Tepecik Eğitim Hastanesi Dergisi, 29(1), 21 - 27. 10.5222/terh.2019.26918 |
| Chicago | ARSLAN YILDIZ AHU Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions. İzmir Tepecik Eğitim Hastanesi Dergisi 29, no.1 (2019): 21 - 27. 10.5222/terh.2019.26918 |
| MLA | ARSLAN YILDIZ AHU Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions. İzmir Tepecik Eğitim Hastanesi Dergisi, vol.29, no.1, 2019, ss.21 - 27. 10.5222/terh.2019.26918 |
| AMA | ARSLAN YILDIZ A Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions. İzmir Tepecik Eğitim Hastanesi Dergisi. 2019; 29(1): 21 - 27. 10.5222/terh.2019.26918 |
| Vancouver | ARSLAN YILDIZ A Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions. İzmir Tepecik Eğitim Hastanesi Dergisi. 2019; 29(1): 21 - 27. 10.5222/terh.2019.26918 |
| IEEE | ARSLAN YILDIZ A "Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions." İzmir Tepecik Eğitim Hastanesi Dergisi, 29, ss.21 - 27, 2019. 10.5222/terh.2019.26918 |
| ISNAD | ARSLAN YILDIZ, AHU. "Investigation of Breast Cancer Cells and Phospholipid Cell Membrane Interactions". İzmir Tepecik Eğitim Hastanesi Dergisi 29/1 (2019), 21-27. https://doi.org/10.5222/terh.2019.26918 |
