Yıl: 2018 Cilt: 22 Sayı: 6 Sayfa Aralığı: 1508 - 1517 Metin Dili: İngilizce DOI: 10.16984/saufenbilder.327153 İndeks Tarihi: 25-10-2019

Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli

Öz:
In this study, Bacillus subtilis akky1 strain was isolated from the soil of beech forest in Akkuş City, OrduProvince, Turkey. akky1 strain was identified by 16S rRNA analysis. The full-length 16S rRNA sequenceof akky1 strain showed the 100% similarity with Bacillus subtilis strain B7 (KC310823.1) . A 642 bp DNAfragment was obtained from genomic DNA using primers designed based on the gene sequence of Bacillussubtilis xylanase given in GenBank. The gene encoding xylanase was cloned into pET28b (+) plasmidvector, sequenced and expressed in Escherichia coli BL21 (DE3). The hexahistidine (6xHis) tagged fusionprotein was purified using nickel affinity chromatography and the xylanase activity was measured. Themolecular mass of the purified xylanase was approximately 26 kDa as estimated by SDS-PAGE. Thexylanase had optimal activity at pH 6.0 and 60°C. The Km values of the recombinant enzyme towardsbeechwood was 3.33 mg/ml.
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APA ŞENTÜRK S, ULUSU Y, KUDUĞ CEYLAN H (2018). Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli. , 1508 - 1517. 10.16984/saufenbilder.327153
Chicago ŞENTÜRK Sema BİLGİN,ULUSU Yakup,KUDUĞ CEYLAN HÜLYA Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli. (2018): 1508 - 1517. 10.16984/saufenbilder.327153
MLA ŞENTÜRK Sema BİLGİN,ULUSU Yakup,KUDUĞ CEYLAN HÜLYA Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli. , 2018, ss.1508 - 1517. 10.16984/saufenbilder.327153
AMA ŞENTÜRK S,ULUSU Y,KUDUĞ CEYLAN H Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli. . 2018; 1508 - 1517. 10.16984/saufenbilder.327153
Vancouver ŞENTÜRK S,ULUSU Y,KUDUĞ CEYLAN H Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli. . 2018; 1508 - 1517. 10.16984/saufenbilder.327153
IEEE ŞENTÜRK S,ULUSU Y,KUDUĞ CEYLAN H "Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli." , ss.1508 - 1517, 2018. 10.16984/saufenbilder.327153
ISNAD ŞENTÜRK, Sema BİLGİN vd. "Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli". (2018), 1508-1517. https://doi.org/10.16984/saufenbilder.327153
APA ŞENTÜRK S, ULUSU Y, KUDUĞ CEYLAN H (2018). Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22(6), 1508 - 1517. 10.16984/saufenbilder.327153
Chicago ŞENTÜRK Sema BİLGİN,ULUSU Yakup,KUDUĞ CEYLAN HÜLYA Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22, no.6 (2018): 1508 - 1517. 10.16984/saufenbilder.327153
MLA ŞENTÜRK Sema BİLGİN,ULUSU Yakup,KUDUĞ CEYLAN HÜLYA Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.22, no.6, 2018, ss.1508 - 1517. 10.16984/saufenbilder.327153
AMA ŞENTÜRK S,ULUSU Y,KUDUĞ CEYLAN H Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2018; 22(6): 1508 - 1517. 10.16984/saufenbilder.327153
Vancouver ŞENTÜRK S,ULUSU Y,KUDUĞ CEYLAN H Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2018; 22(6): 1508 - 1517. 10.16984/saufenbilder.327153
IEEE ŞENTÜRK S,ULUSU Y,KUDUĞ CEYLAN H "Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli." Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22, ss.1508 - 1517, 2018. 10.16984/saufenbilder.327153
ISNAD ŞENTÜRK, Sema BİLGİN vd. "Cloning, Expression and Characterization of Xylanase (xyn-akky1) from Bacillus subtilis in Escherichia coli". Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22/6 (2018), 1508-1517. https://doi.org/10.16984/saufenbilder.327153