Yıl: 2018 Cilt: 22 Sayı: 6 Sayfa Aralığı: 1674 - 1682 Metin Dili: İngilizce DOI: 10.16984/saufenbilder.395016 İndeks Tarihi: 30-10-2019

Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats

Öz:
The genus Bacillus is frequently found in soil, water, and food. Bacillus cereus and Bacillus anthracis arethe main pathogens causing foodborne diseases and serious infections in humans. A total of 52 Bacillusspp. from meat samples was tested for determination of biofilm production, antimicrobial resistance pattern,and beta-lactamase activity. The 24 (46.1%) Bacillus isolates were found to be for biofilm production. Ofthe 24 (46.1%) biofilm producer Bacillus isolates, 13 (25%), 6 (11.5%) and 5 (9.6%) were considered asstrong, moderate and weak biofilm producer, respectively. The most common species for the production ofbiofilm was Bacillus thuringiensis (80%). Antimicrobial disk susceptibility tests of Bacillus spp. revealedhigh resistance to ampicillin (84.6%) followed by penicillin (75%), cefepime (34.6%), and cefoxitin(26.9%). A multidrug resistance to at least 3 or more antimicrobials was observed in the 25 isolates (48.1%).All Bacillus spp. were sensitive to vancomycin, gentamicin, amikacin, ciprofloxacin, and imipenem. Thesusceptibility rate to streptomycin, chloramphenicol, and trimethoprim-sulphamethoxazole was 94.2%.Among the isolates, the 6 (11.5%) isolates were found to be sensitive to all antimicrobial agents tested.Besides, only one isolate from meat was found to be positive for beta-lactamase test. The existence ofbiofilm production as a virulence factor and of multidrug resistance in bacteria isolated from food shouldnot be underestimated in terms of food safety, public health, and economic concerns.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • W. E. Farrar and A. C. Reboli, “The Genus Bacillus-Medical”. In: Dworkin, M, Falkow S. Rosenberg E, Stackebrandt E, Schleifer KH. (Eds.), The Prokaryotes, vol.4. Springer, Minneapolis, pp. 609-630, 2006.
  • A. K. Bhunia, “Foodborne microbial pathogens: mechanisms and pathogenesis”, Springer, New York, 2008.
  • N. A. Logan, A. R. Hoffmaster, S. V. Shadomy, K. E. Stauffer, “Bacillus and other aerobic endospore forming bacteria”. In: Versalovic J, Carroll, KC, Funke G, Jorgensen JH, Landry, ML, Warnock DW. (Eds.), Manual of Clinical Microbiology, 10th edn, Vol. 1, Washington DC, American Society for Microbiology, pp. 381–402. 2011.
  • L. V. Poulsen, “Microbial biofilm in food processing”, Lebensmittel-Wissenschaft and Technologie, vol. 32, pp. 321-326, 1999.
  • R. M. Donlan and J. W. Costerton, “Biofilms: survival mechanisms of clinically relevant microorganisms”, Clinical Microbiology Reviews, vol. 15, pp. 167-193, 2002.
  • A. Cherif-Antar, B. Moussa Boudjemaa, N. Didouh, K. Medjahdi, B. Mayo, and A. B. Florez, “Diversity and biofilm-forming capability of bacteria recovered from stainless steel pipes of a milk-processing dairy plant”, Dairy Science and Technology, vol. 96, pp. 27–38, 2016.
  • T. F. Mah and G. A. O’Toole, “Mechanisms of biofilm resistance to antimicrobial agents”, Trends in Microbiology, vol. 9, pp. 34-39, 2001.
  • J. D. Brooks and S. H. Flint, “Biofilms in the food industry: problems and potential solutions”, International Journal of Food Science and Technology, vol. 43 no. 12, pp. 2163–2176, 2008.
  • P. F. McDermott, S. Zhao, D. D. Wagner, S. Simjee, R. D. Walker, and D. G. White, “The food safety perspective of antibiotic resistance”, Animal Biotechnology, 13:71– 84. 2002.
  • J. Romero, C. G. Feijoó, and P. Navarrete, “Antibiotics in Aquaculture – Use, Abuse and Alternatives”, Health and Environment in Aquaculture, 10.5772/28157, 2012.
  • C. U. Tuazon, H. W. Murray, C. Levy, M. N. Solny, J. A. Curtin, and J. N. Sheagren, “Serious infections from Bacillus sp.”, Journal of the American Medical Association, 241:1137-1140, 1979.
  • D. Djordjevic, M. Wiedmann, and L. A. McLandsborough, “Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation”, Applied and Environmental Microbiology, vol. 68, pp. 2950-2958, 2002.
  • S. Stepanovic, D. Vukovic, I Dakic, B. Savic, and M. Svabic-Vlahovic, “A modified microtiter-plate test for quantification of staphylococcal biofilm formation”, Journal of Microbiological Methods, vol.40, pp. 175–179, 2000.
  • Clinical and Laboratory Standards Institute. “Performance standards for antimicrobial disk susceptibility tests; approved standard- 11th ed CLSI document M02-A1”, vol. 32, no.1, Clinical and Laboratory Standards Institute, Wayne, PA, 2012.
  • T. R. Oberhofer and D. W. Towle, “Evaluation of the rapid penicillinase paper strip test for detection of beta-lactamase” Journal of Clinical Microbiology, vol.15, pp. 196-199, 1982.
  • R. Kuroki , K. Kawakami , L. Qin, C. Kaji, K. Watanabe, Y. Kimura, C. Ishiguro, S. Tanimura, Y. Tsuchiya, I. Hamaguchi, M. Sakakura, S. Sakabe S, K. Tsuji, M. Inoue, and H. Watanabe, “Nosocomial bacteremia caused by biofilm-forming Bacillus cereus and Bacillus thuringiensis”, Internal Medicine Journal, vol. 48, pp. 791–796, 2009.
  • M. Morikawa, S. Kagihiro, M. Haruki, K. Takano, S. Branda, R. Kolter, and S. Kanaya, “Biofilm formation by a Bacillus subtilis strain that produces ɣpolyglutamate”, Microbiology, vol.152, pp. 2801-2807, 2006.
  • R. Majed, C. Faille, M. Kallassy, and M. Gohar, “Bacillus cereus biofilms-same only different”, Frontiers in Microbiology, vol.7, 1054. doi: 10.3389/fmicb.2016.01054, 2016.
  • H. Y. Done, A. K. Venkatesan, and R. U. Halden, “Does the recent growth of aquaculture create antibiotic resistance threats different from those associated with land animal production in agriculture?”, Journal of the American Association of Pharmaceutical Scientists, vol.17, no.3, pp. 513-24, 2015.
  • O. Kursun, A. Guner, and G. Ozmen, “Prevalence of Bacillus cereus in rabbit meat consumed in Burdur-Turkey, its enterotoxin producing ability and antibiotic susceptibility”, Kafkas Universitesi Veteriner Fakultesi Dergisi, vol. 17 (Suppl A): S31-S35, 2011.
  • . H. Yim, K. Y. Kim, J. W. Chon, D. H. Kim, H. S. Kim, D. S. Choi, I. S. Choi, and K. H. Seo, “Incidence, antibiotic susceptibility, and toxin profiles of Bacillus cereus sensu lato isolated from Korean fermented soybean products”, Journal of Food Science, vol.80, no.6, M1266-67, 2015.
  • I. Chaabouni, I. Barkallah, C. Hamdi, A. Jouii, M. Saidi, J. Mahillon, and A. Cherif, “Metabolic capacities and toxigenic potential as key drivers of Bacillus cereus ubiquity and adaptation”, Annals of Microbiology, vol. 65, pp. 975-983, 2015.
  • G. M. Noor Uddin, M. H. Larsen, H. Christensen, F. M. Aarestrup, T. M. Phu, and A. Dalsgaard, “Identification and antimicrobial resistance of bacteria isolated from probiotic products used in shrimp culture”, Plos One vol. 10, no. 7, e0132338. https://doi.org/10.1371/journal.pone.01323 38, 2015.
  • B. A. Mohammadou, G. Le Blay, C. M. Mbofung, and G. Barbier, “Antimicrobial activities, toxinogenic potential and sensitivity to antibiotics of Bacillus strains isolated from Mbuja, an Hibiscus sabdariffa fermented seeds from Cameroon”, African Journal of Biotechnology, vol.13, no. 35, pp. 3617-3627, 2014.
  • M. Ikeda, Y. Yagihara, K. Tatsuno, M. Okazaki, S. Okugawa, and K. Moriya, “Clinical characteristics and antimicrobial susceptibility of Bacillus cereus blood stream infection”, Annals of Clinical Microbiology and Antimicrobials, vol.14, 43, doi 10.1186/s12941-015-0104-2, 2015.
APA OZDEMIR F, ARSLAN S (2018). Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats. , 1674 - 1682. 10.16984/saufenbilder.395016
Chicago OZDEMIR FATMA,ARSLAN SEZA Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats. (2018): 1674 - 1682. 10.16984/saufenbilder.395016
MLA OZDEMIR FATMA,ARSLAN SEZA Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats. , 2018, ss.1674 - 1682. 10.16984/saufenbilder.395016
AMA OZDEMIR F,ARSLAN S Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats. . 2018; 1674 - 1682. 10.16984/saufenbilder.395016
Vancouver OZDEMIR F,ARSLAN S Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats. . 2018; 1674 - 1682. 10.16984/saufenbilder.395016
IEEE OZDEMIR F,ARSLAN S "Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats." , ss.1674 - 1682, 2018. 10.16984/saufenbilder.395016
ISNAD OZDEMIR, FATMA - ARSLAN, SEZA. "Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats". (2018), 1674-1682. https://doi.org/10.16984/saufenbilder.395016
APA OZDEMIR F, ARSLAN S (2018). Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22(6), 1674 - 1682. 10.16984/saufenbilder.395016
Chicago OZDEMIR FATMA,ARSLAN SEZA Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22, no.6 (2018): 1674 - 1682. 10.16984/saufenbilder.395016
MLA OZDEMIR FATMA,ARSLAN SEZA Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.22, no.6, 2018, ss.1674 - 1682. 10.16984/saufenbilder.395016
AMA OZDEMIR F,ARSLAN S Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2018; 22(6): 1674 - 1682. 10.16984/saufenbilder.395016
Vancouver OZDEMIR F,ARSLAN S Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2018; 22(6): 1674 - 1682. 10.16984/saufenbilder.395016
IEEE OZDEMIR F,ARSLAN S "Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats." Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22, ss.1674 - 1682, 2018. 10.16984/saufenbilder.395016
ISNAD OZDEMIR, FATMA - ARSLAN, SEZA. "Biofilm Production and Antimicrobial Susceptibility Profiles of Bacillus spp. from Meats". Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22/6 (2018), 1674-1682. https://doi.org/10.16984/saufenbilder.395016