Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations
Yıl: 2018 Cilt: 15 Sayı: 3 Sayfa Aralığı: 370 - 375 Metin Dili: İngilizce DOI: 10.4274/tjps.37132 İndeks Tarihi: 22-02-2020
Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations
Öz: Objectives: The aim of this study was to prepare pharmaceutical formulations of mouthwashes and to examine the antimicrobial activities ofessential oils obtained from plants used traditionally in Turkey for oral infections.Materials and Methods: Essential oils were obtained from herbal drugs using water distillation with Clevenger apparatus. The antimicrobialcapacities of mouthwash formulations containing a mixture of essential oils with proportions of 4.5% and 9.0% were examined using disc diffusionand microbroth dilutions.Results: The inhibition zone diameters were determined to vary between 7 and 59 mm. The static and cidal activity was generally 50% and greaterthan 50% when pure essential oil samples were applied on microorganism specimens. Formulation F2, which contained a mixture of essential oilswith proportions of 4.5%, showed 6.25% minimum bactericidal effect on Staphylococcus aureus ATCC 25923, and 3.125% the minimum inhibitoryconcentration and minimum bactericidal concentration on all other microorganisms. The antimicrobial effect of pure essential oil samples appliedon microorganisms was lower than of mouthwashes formulations; the antimicrobial effect of F2, which contained a mixture of essential oils withproportions of 4.5% was higher than formulation F1, which contained a mixture of essential oils with proportions of 9%.Conclusion: The results obtained by these methods allow us to conclude that the essential oils and the prepared F1 and F2 mouthwash formulationsexerted activity against microorganisms affecting the oral cavity. The F2 formulation also had significant antimicrobial activity on the testedmicroorganisms.
Anahtar Kelime: Konular:
Uçucu Yağ İçeren Bazı Bitkilerin ve Gargara Formülasyonlarının Antimikrobiyal Aktivitelerinin Araştırılması
Öz: Amaç: Türkiye’de halk arasında yaygın olarak kullanılan bitkilerden elde edilen uçucu yağların antimikrobiyal etkilerini araştırmak ve bu etkilerden yola çıkarak ağız enfeksiyonlarında kullanılmak üzere gargara formülasyonu hazırlamaktır. Gereç ve Yöntemler: Uçucu yağlar, bitkilerden (Origanum vulgare L. ssp. hirtum, Rosmarinus officinalis L., Laurus nobilis L., Salvia fruticosa Mill.) su distilasyonu metodu ile Clevenger apareyi kullanılarak elde edilmiştir. Elde edilen uçucu yağlar ve %4.5 ve %9.0 oranlarında uçucu yağların karışımını içeren gargara formülasyonlarının antimikrobiyal aktiviteleri disk difüzyon metodu ve mikrobroth dilüsyon metodu ile araştırılmıştır. Bulgular: Bu çalışmada antimikrobiyal aktivite disk difüzyon metodu ve mikrobroth dilüsyon metoduyla değerlendirilmiştir. İnhibisyon zon çaplarının 7-59 mm arasında değişkenlik gösterdiği belirlenmiştir. Uçucu yağlar, mikroorganizmalar üzerine saf olarak uygulandığında statik ve sidal etkinliğin genellikle %50 ve %50’nin üzerinde olduğu gözlenmiştir. %4.5 oranında uçucu yağ karışımı içeren F2 formülasyonunun Staphylococcus aureus ATCC 25923 üzerindeki minimum bakterisidal etkisinin %6.25 olduğu ve diğer tüm mikroorganizmalar üzerinde minimum inhibitör konsantrasyon ve minimum bakterisidal konsantrasyonunun %3.125 olduğu bulunmuştur. Uçucu yağ örneklerinin mikroorganizmalar üzerine saf olarak uygulandığında gösterdikleri antimikrobiyal etkinin, gargara formülasyonlarına göre daha düşük olduğu; ayrıca %4.5 oranında uçucu yağ karışımı içeren F2 formülasyonunun, %9.0 oranında uçucu yağ karışımı içeren F1 formülasyonuna göre test edilen tüm mikroorganizmalar üzerine daha yüksek antimikrobiyal etkiye sahip olduğu bulunmuştur. Sonuç: Elde edilen sonuçlar uçucu yağların karışımı ile hazırlanan F1 ve F2 formülasyonlarının, test edilen mikroorganizmalara karşı antimikrobiyal etkiye sahip olduğunu ortaya koymuştur. Ayrıca çalışma sonucunda F2 formülasyonunun test edilen mikroorganizmalar üzerinde önemli oranda antimikrobiyal aktiviteye sahip olduğu rapor edilmiştir.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | KULAKSIZ B, ER S, ÜSTÜNDAĞ OKUR N, SALTAN İŞCAN G (2018). Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations. , 370 - 375. 10.4274/tjps.37132 |
| Chicago | KULAKSIZ BÜŞRA,ER SEVDA,ÜSTÜNDAĞ OKUR NESLİHAN,SALTAN İŞCAN Gülçin Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations. (2018): 370 - 375. 10.4274/tjps.37132 |
| MLA | KULAKSIZ BÜŞRA,ER SEVDA,ÜSTÜNDAĞ OKUR NESLİHAN,SALTAN İŞCAN Gülçin Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations. , 2018, ss.370 - 375. 10.4274/tjps.37132 |
| AMA | KULAKSIZ B,ER S,ÜSTÜNDAĞ OKUR N,SALTAN İŞCAN G Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations. . 2018; 370 - 375. 10.4274/tjps.37132 |
| Vancouver | KULAKSIZ B,ER S,ÜSTÜNDAĞ OKUR N,SALTAN İŞCAN G Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations. . 2018; 370 - 375. 10.4274/tjps.37132 |
| IEEE | KULAKSIZ B,ER S,ÜSTÜNDAĞ OKUR N,SALTAN İŞCAN G "Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations." , ss.370 - 375, 2018. 10.4274/tjps.37132 |
| ISNAD | KULAKSIZ, BÜŞRA vd. "Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations". (2018), 370-375. https://doi.org/10.4274/tjps.37132 |
| APA | KULAKSIZ B, ER S, ÜSTÜNDAĞ OKUR N, SALTAN İŞCAN G (2018). Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations. Turkish Journal of Pharmaceutical Sciences, 15(3), 370 - 375. 10.4274/tjps.37132 |
| Chicago | KULAKSIZ BÜŞRA,ER SEVDA,ÜSTÜNDAĞ OKUR NESLİHAN,SALTAN İŞCAN Gülçin Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations. Turkish Journal of Pharmaceutical Sciences 15, no.3 (2018): 370 - 375. 10.4274/tjps.37132 |
| MLA | KULAKSIZ BÜŞRA,ER SEVDA,ÜSTÜNDAĞ OKUR NESLİHAN,SALTAN İŞCAN Gülçin Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations. Turkish Journal of Pharmaceutical Sciences, vol.15, no.3, 2018, ss.370 - 375. 10.4274/tjps.37132 |
| AMA | KULAKSIZ B,ER S,ÜSTÜNDAĞ OKUR N,SALTAN İŞCAN G Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations. Turkish Journal of Pharmaceutical Sciences. 2018; 15(3): 370 - 375. 10.4274/tjps.37132 |
| Vancouver | KULAKSIZ B,ER S,ÜSTÜNDAĞ OKUR N,SALTAN İŞCAN G Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations. Turkish Journal of Pharmaceutical Sciences. 2018; 15(3): 370 - 375. 10.4274/tjps.37132 |
| IEEE | KULAKSIZ B,ER S,ÜSTÜNDAĞ OKUR N,SALTAN İŞCAN G "Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations." Turkish Journal of Pharmaceutical Sciences, 15, ss.370 - 375, 2018. 10.4274/tjps.37132 |
| ISNAD | KULAKSIZ, BÜŞRA vd. "Investigation of Antimicrobial Activities of Some Herbs Containing Essential Oils and Their Mouthwash Formulations". Turkish Journal of Pharmaceutical Sciences 15/3 (2018), 370-375. https://doi.org/10.4274/tjps.37132 |
