In this research, it was aimed to determine the preparedness levels of pre-service teachers for teaching profession regarding various variables. The research using the survey model was conducted with 192 pre-service teachers studying in science and biology teacher education departments in the academic year of 2017-2018. The research focus on investigating variables of gender, choices of master science, grade level and mean of academic achievement on preparedness to teach of pre-service teachers’. Preparedness to Teach Scale was used as data collection. The scale is based on a 5-point Likert type consisting of 4 sub-factors and 20 questions, namely "Effective learning", "Teaching process design","Technopedagogical competence" and "Understanding of learning". The questions in question; 1=very insufficient, 5=very sufficient. In this study, the reliability of the scale was determined by Cronbach's alpha coefficient (α=.92), scale-forming effective learning (α=.83) designing the teaching process (α=.84), technopedagogical competence (α=.82) and learning comprehension (α=.64). It was determined that the level of pre-service teacher' preparedness to the teaching profession, the main science department in which they are studying, the willingness to come to the main science branch, the class level and the language grade average variables are statistically different. In terms of the gender variable, it was determined that there was no statistically significant difference in the level of preparedness of the pre-service teachers to the teaching profession. All of the sub-factors of the scale used in the study were determined as a variable that makes a meaningful difference in grade level.