Aim: Viruses are important causes of acute gastroenteritis for children. Statistics related to the epidemiology of gastroenteritis caused by rotavirus and adenovirus vary according to regions. This study aims to specify the frequency of adenovirus and rotavirus for pediatric patients with acute gastroenteritis who apply the Medical Faculty of Kafkas University. Material and Method: The records of the feces samples of 3763 patients with the preliminary diagnosis of acute gastroenteritis between October 10th, 2013 and October 30th, 2018 were examined retrospectively. The presence of adenovirus and rotavirus antigens was investigated with a qualitative immunochromatographic test. The laboratory data of the cases were analyzed by using SPSS (Statistical Package for Social Sciences) 16.0 program. For statistical significance, p<0.05 was considered. Results: The viral antigens were positive for 744 out of 3763 (20%) patients. It has been identified that 549 of them (74%) are rotavirus, 53 of them (7%) are adenovirus and 142 of them (19%) are both rotavirus and adenovirus positive. Among those viral antigen positive patients, 355 (48%) of them were female, and 389 (52%) of them were male. It has been found that the antigen positiveness (% 84) is most frequent in the 0–2 age group and when being analyzed in terms of seasons, it is more frequent in winter and spring. Conclusion: In addition to parasitological and bacteriological examinations of stool samples, controlling the viral antigens is essential determining the etiological agent and treatment for childhood acute gastroenteritis infections. In the symptomatic treatment of viral gastroenteritis, we aimed to draw attention to the widespread use of rotavirus vaccine in the community to prevent the use of empirical antibiotics and to reduce rotavirus gastroenteritis.