THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION

Akaltun, Mazlum Serdar; PEHLİVAN, MUSTAFA; Nursal, Ayse Feyda; pehlivan, sacide; OZDILLI, KÜRSAT; Gursoy, Savas

doi:10.26650/IUITFD.2019.0023

THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION

Sacide PEHLİVAN, (İstanbul Üniversitesi)

Savaş GÜRSOY, (Gaziantep Üniversitesi)

Ayşe Feyda NURSAL, (Hitit Üniversitesi)

Mazlum Serdar AKALTUN, (Gaziantep Üniversitesi)

Kürşat ÖZDİLLİ, (Medipol Üniversitesi)

Mustafa PEHLİVAN (Gaziantep Üniversitesi)

İstanbul Tıp Fakültesi Dergisi

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Yıl: 2020 Cilt: 83 Sayı: 2 Sayfa Aralığı: 81 - 85 Metin Dili: İngilizce DOI: 10.26650/IUITFD.2019.0023 İndeks Tarihi: 27-04-2020

THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION

Öz:

Objective: Ankylosing spondylitis (AS) is a chronic inflammatorydisorder. MicroRNAs (miRNAs) can function as either oncogenesor tumor suppressor genes. Altered miRNA expression has beenimplicated in the pathogenesis of several diseases. Therefore,we aimed to explore the effects of miR-196a2T/C (rs11614913)variant profile on susceptibility to AS in a Turkish population.Materials and Methods: Blood samples were collected from 78AS patients and 79 healthy controls. miR-196a2T/C variant wasgenotyped by PCR-RFLP. Odds ratio (OR) with 95% confidenceinterval (95%CI) were calculated using the χ2 test.Results: The frequency of T/C and T/T genotypes of the miR-196a2T/C were higher in AS patients compared to healthy controls(p=0.034 and p=0.028, respectively). The subjects carryingthe miR-196a2T/C variant T/T genotype showed a 2.542-foldincreased AS risk than the control group. However, no differencewas observed in the allele frequencies of miR-196a2T/Cbetween AS patients and the controls. It was found that C/Cgenotype of miR-196a2T/C variant was more frequent in ASpatients with enthesitis than AS patients without enthesitis(p=0.042). factor for increased susceptibility to AS and is associated withenthesitis in the Turkish population.

Anahtar Kelime:

TÜRK POPÜLASYONUNDA ANKİLOZAN SPONDİLİTE YATKINLIK SAĞLAYICI FAKTÖR OLARAK miR-196a2T/C VARYANTI

Öz:

Amaç: Ankilozan spondilit (AS) kronik, enflamatuar bir hastalıktır. MikroRNA’lar (miRNA’,lar) onkogen veya tümör baskılayıcı genler olarak fonksiyon yapabilirler. Çeşitli hastalıkların patogenezinde miRNA ekspresyonu’nun değişmesi bulunmaktadır. Bu nedenle, Türk toplumunda AS yatkınlığında miR-196a2T/C (rs11614913) varyant profilinin etkilerini araştırmayı amaçladık. Gereç ve Yöntem: 78 AS hastası ve 79 kontrolden kan örnekleri toplandı. miR-196a2T/C varyantı PCR-RFLP ile genotiplendi. χ2 testi kullanılarak %95 güven aralığı ile Odds oranı (OR) hesaplandı. Bulgular: miR-196a2T/C T/C ve T/T genotipleri AS hastalarında sağlıklı kontrollere kıyasla daha yüksekti (sırasıyla, p=0.034 and p=0.028). miR-196a2T/C varyant T/T genotipini taşıyan kişiler kontrol grubundan 2,542 kat artmış AS riski gösterdiler. Ancak, miR-196a2T/C allele frekansları açısından AS hastaları ve kontroller arasında fark saptanmadı. miR-196a2T/C varyant C/C genotipinin entezitli AS hastalarında entezit bulunmayan AS hastalarından daha çok olduğu bulundu (p=0.042). Sonuç: miR-196a2T/C varyantı Türk toplumunda AS ve hastalığın entezit ile ilişkisine yatkınlık sağlayan genetik risk faktörü rolü göstermektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	pehlivan s, Gursoy S, Nursal A, Akaltun M, OZDILLI K, PEHLİVAN M (2020). THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. , 81 - 85. 10.26650/IUITFD.2019.0023
Chicago	pehlivan sacide,Gursoy Savas,Nursal Ayse Feyda,Akaltun Mazlum Serdar,OZDILLI KÜRSAT,PEHLİVAN MUSTAFA THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. (2020): 81 - 85. 10.26650/IUITFD.2019.0023
MLA	pehlivan sacide,Gursoy Savas,Nursal Ayse Feyda,Akaltun Mazlum Serdar,OZDILLI KÜRSAT,PEHLİVAN MUSTAFA THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. , 2020, ss.81 - 85. 10.26650/IUITFD.2019.0023
AMA	pehlivan s,Gursoy S,Nursal A,Akaltun M,OZDILLI K,PEHLİVAN M THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. . 2020; 81 - 85. 10.26650/IUITFD.2019.0023
Vancouver	pehlivan s,Gursoy S,Nursal A,Akaltun M,OZDILLI K,PEHLİVAN M THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. . 2020; 81 - 85. 10.26650/IUITFD.2019.0023
IEEE	pehlivan s,Gursoy S,Nursal A,Akaltun M,OZDILLI K,PEHLİVAN M "THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION." , ss.81 - 85, 2020. 10.26650/IUITFD.2019.0023
ISNAD	pehlivan, sacide vd. "THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION". (2020), 81-85. https://doi.org/10.26650/IUITFD.2019.0023

APA	pehlivan s, Gursoy S, Nursal A, Akaltun M, OZDILLI K, PEHLİVAN M (2020). THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. İstanbul Tıp Fakültesi Dergisi, 83(2), 81 - 85. 10.26650/IUITFD.2019.0023
Chicago	pehlivan sacide,Gursoy Savas,Nursal Ayse Feyda,Akaltun Mazlum Serdar,OZDILLI KÜRSAT,PEHLİVAN MUSTAFA THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. İstanbul Tıp Fakültesi Dergisi 83, no.2 (2020): 81 - 85. 10.26650/IUITFD.2019.0023
MLA	pehlivan sacide,Gursoy Savas,Nursal Ayse Feyda,Akaltun Mazlum Serdar,OZDILLI KÜRSAT,PEHLİVAN MUSTAFA THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. İstanbul Tıp Fakültesi Dergisi, vol.83, no.2, 2020, ss.81 - 85. 10.26650/IUITFD.2019.0023
AMA	pehlivan s,Gursoy S,Nursal A,Akaltun M,OZDILLI K,PEHLİVAN M THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. İstanbul Tıp Fakültesi Dergisi. 2020; 83(2): 81 - 85. 10.26650/IUITFD.2019.0023
Vancouver	pehlivan s,Gursoy S,Nursal A,Akaltun M,OZDILLI K,PEHLİVAN M THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION. İstanbul Tıp Fakültesi Dergisi. 2020; 83(2): 81 - 85. 10.26650/IUITFD.2019.0023
IEEE	pehlivan s,Gursoy S,Nursal A,Akaltun M,OZDILLI K,PEHLİVAN M "THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION." İstanbul Tıp Fakültesi Dergisi, 83, ss.81 - 85, 2020. 10.26650/IUITFD.2019.0023
ISNAD	pehlivan, sacide vd. "THE miR-196a2T/C VARIANT AS A POSSIBLE PREDISPOSING FACTOR FOR ANKYLOSING SPONDYLITIS IN A TURKISH POPULATION". İstanbul Tıp Fakültesi Dergisi 83/2 (2020), 81-85. https://doi.org/10.26650/IUITFD.2019.0023