Aim: In current studies, it is reported that exposure to electromagneticfield (EMF) is related to numerous illnesses. Important parts of our bodies,oral and dental tissues can also be influenced by the EMF. In this study,it was aimed to determine possible negative impacts of EMFs on dentalenamel hardness and to test the efficiency of the antioxidant agentsagainst possible adverse effects.Methodology: In this study, 4-month-old 32 Winster strain Albino ratswere used, each weighing 250-300 gr in average. In total, 40 teeth wereused from these 32 rats. These teeth were classified into four groups.Group 1 was determined as the control group, and Group 2,3, and 4 wereexposed to EMF measured as 2.48 μT magnetic field intensity and 80.3 V/ m electric field intensity for 52 days. Ganodermalucidum (20 mg/kg/day) was applied to the Group 3 together with the EMF. Melatonin (10mg/kg/ day) was applied to the Group 4 together with the EMF. The teethpulled out after the euthanasia were embedded into acrylic blocks andmicro-hardness test was applied. The normality of the variables wasanalyzed through the Shapiro-Wilk test. After the homogeneity test, onewayanalysis of variance (ANOVA) was used to compare surface microhardness(p<0.05).Results: As a result of the conducted statistical analyses, there wasstatistically no significant difference among the groups concerning microhardness(p>0.05).Conclusions: It was determined in our study that 52-day exposure to2.48 μT EMF did not cause any micro-hardness change in the toothenamels of the rats. It was also observed that the used antioxidant agentsdid not cause any significant difference.