Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi
Yıl: 2019 Cilt: 8 Sayı: 4 Sayfa Aralığı: 438 - 443 Metin Dili: Türkçe İndeks Tarihi: 03-04-2020
Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi
Öz: Glutatyon redüktaz (Glutatyon: NADP+oksidoredüktaz, EC 1.8.1.7; GR) düşük (GSH) veyayüksek molekül ağırlıklı (GSSG) disülfit substratları ileindirgenmiş piridin nükleotidleri (NADPH) arasındaelekton transferini katalizler. GR inhibitörleri sonzamanlarda anti-sıtma ve kansere karşı faaliyetlerininkeşfedilmesi nedeniyle oldukça popüler hale gelmiştir.Enzimatik aktivite ölçümü Beutler metodu vespektrofotometre kullanılarak 25°C’de yapıldı. Ölçümsistemi 100 mM Tris-HCl tamponunda (pH 8;0), 0,5mM EDTA, 3,3 mM GSSG ve 0,1 mM NADPHiçeriyordu. Nitrobenzen (1), 3,5-dinitrosalisilik asit (2),(2,4-dinitrofenil) ((4-(prop-1-en-2-yl) sikloheks-1-enil)-metil) sulfan (3), ((7,7-dimetilbisiklo [2.2.1]heptan-1-il)metil)-(2,4-dinitrofenil)sulfan (4) ve ((2-kloro-7,7-dimetilbisiklo [2.2.1] heptan-1-il)metil)-(2,4-dinitrofenil)sulfan (5) maddelerinin GR enzimiüzerindeki in vitro inhibisyon etkisi incelendi.İnhibisyon etkisi gösteren maddeler için % aktivite-[I] grafikleri çizilerek IC50 değerleri bulunmuştur. Bumaddelerin GR için IC50 değerlerinin mikromolarseviyesinde olduğu gözlemlendi.
Anahtar Kelime: Konular:
Investigation of Inhibition Effect of Nitro Group Containing Substances on Glutathione Reductase Enzyme
Öz: Glutathione reductase (Glutathione: NADP+ oxidoreductase, EC 1.8.1.7; GR) catalyzes electron transfer between reduced (GSH) or high molecular weight (GSSG) disulfide substrates and reduced pyridine nucleotides (NADPH). Discovery of glutathione reductase (GR) inhibitors has become very popular recently due to antimalarial and anticancer activities. Enzymatic activity was measured by Beutler’s method with a Spectrophotometer, at 25°C. The assay system contained 100 mM Tris-HCl buffer pH 8.0, including 0.5 mM EDTA, 3.3 mM GSSG and 0.1 mM NADPH. Inhibition effects of Nitrobenzene (1), 3,5- Dinitrosalicylic acid (2), (2,4-Dinitrophenyl)((4-(prop1-en-2-yl)cyclohex-1-enyl)-methyl)sulfane (3), ((7,7- dimethylbicyclo[2.2.1]heptan-1-yl)methyl)-(2,4- dinitrophenyl)sulfane (4), and ((2-Chloro-7,7- dimethylbicyclo[2.2.1]heptan-1-yl)methyl)-(2,4- dinitrophenyl)sulfane (5) compounds on GR have been investigated in vitro. IC50 values were determined by drawing % activity- [I] graphs for compounds showing inhibition effects. IC50 values of GR for these substances were observed to be at micromolar level.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | KAYA A, ŞENTÜRK M, ÇELENK KAYA E (2019). Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi. , 438 - 443. |
| Chicago | KAYA AFŞİN AHMET,ŞENTÜRK MURAT,ÇELENK KAYA ELİF Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi. (2019): 438 - 443. |
| MLA | KAYA AFŞİN AHMET,ŞENTÜRK MURAT,ÇELENK KAYA ELİF Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi. , 2019, ss.438 - 443. |
| AMA | KAYA A,ŞENTÜRK M,ÇELENK KAYA E Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi. . 2019; 438 - 443. |
| Vancouver | KAYA A,ŞENTÜRK M,ÇELENK KAYA E Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi. . 2019; 438 - 443. |
| IEEE | KAYA A,ŞENTÜRK M,ÇELENK KAYA E "Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi." , ss.438 - 443, 2019. |
| ISNAD | KAYA, AFŞİN AHMET vd. "Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi". (2019), 438-443. |
| APA | KAYA A, ŞENTÜRK M, ÇELENK KAYA E (2019). Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi, 8(4), 438 - 443. |
| Chicago | KAYA AFŞİN AHMET,ŞENTÜRK MURAT,ÇELENK KAYA ELİF Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi 8, no.4 (2019): 438 - 443. |
| MLA | KAYA AFŞİN AHMET,ŞENTÜRK MURAT,ÇELENK KAYA ELİF Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi, vol.8, no.4, 2019, ss.438 - 443. |
| AMA | KAYA A,ŞENTÜRK M,ÇELENK KAYA E Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi. 2019; 8(4): 438 - 443. |
| Vancouver | KAYA A,ŞENTÜRK M,ÇELENK KAYA E Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi. Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi. 2019; 8(4): 438 - 443. |
| IEEE | KAYA A,ŞENTÜRK M,ÇELENK KAYA E "Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi." Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi, 8, ss.438 - 443, 2019. |
| ISNAD | KAYA, AFŞİN AHMET vd. "Nitro grubu İçeren Bazı Maddelerin Glutatyon Redüktaz Enzimi Üzerindeki İnhibisyon Etkisinin İncelenmesi". Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi 8/4 (2019), 438-443. |
