Objective: Acute cholecystitis is one of the common conditions encountered in emergency services and general surgery outpatient clinics. Survival and mortality rates positively correlate with the severity of inflammation. In this study, we aimed to compare established markers of inflammation to the neutrophil/lymphocyte ratio, platelet/lymphocyte ratio and the mean platelet volume and to assess which were the most clinically relevant. Methods: Patients who underwent cholecystectomy in our clinic between June 2012 and June 2017 were retrospectively reviewed. Study subjects were classified into two groups: Group 1, patients with acute cholecystitis and Group 2, patients without acute cholecystitis. Two groups were compared to each other in the length of hospital stay, CRP concentrations, leukocyte counts, neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), red blood cell distribution width, and the mean platelet volume. Results: The length of hospital stay was significantly longer in the Group 1 compared to the Group 2 (p<0.001). No significant differences were found between the groups in the MPV and RDW (p=0.128 and p=0.707, respectively). The NLR, PLR,leukocyte and C-reactive protein were statistically significantly higher in the Group 1 compared to the Group 2 ( all; p<0.001). Conclusion: Our study failed to demonstrate clinical relevance of RDW and MPV values as inflammation markers. We would like to emphasize that the NLR and PLR may be at least as helpful as other routine tests in determining the severity of inflammation.