Fikriye Yasemin ÖZATİK
(Kütahya Sağlık Bilimler Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Kütahya, Türkiye)
Orhan ÖZATİK
(Kütahya Sağlık Bilimleri Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Kütahya, Türkiye)
Semra YİĞİTASLAN
(Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Eskişehir, Türkiye)
Bilgin KAYGISIZ
(Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Eskişehir, Türkiye)
Kevser EROL
(Eskişehir Osmangazi Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Eskişehir, Türkiye)
Yıl: 2020Cilt: 52Sayı: 1ISSN: 1308-8734 / 1308-8742Sayfa Aralığı: 6 - 11İngilizce

31 0
Do Resveratrol and Dehydroepiandrosterone Increase Diminished Ovarian Reserve?
Objective: In this study, the aim is to observe changes induced by dehydroepiandrosterone (DHEA) and resveratrol (RES) in diminished ovarian follicles that was induced by 4-vinylcyclohexenediepoxide (VCD). Materials and Methods: Twenty four Wistar albino female rats were divided into 3 groups: control, DHEA and RES. Unilateral oophorectomy was performed in control group to remove the right ovary of 4 rats and the left ovary of 4 rats. After administration of 160 mg/kg VCD, remaining ovaries were removed. Following the same VCD treatment, in DHEA and RES groups, 60 mg/kg DHEA and 20 mg/kg RES were given for 45 days respectively and residual ovaries were removed. Hematoxylin-eosin and TUNEL staining were performed. Follicle stimulating hormone (FSH), estradiol (E2) and anti-mullerian hormone (AMH) values were measured. Results: In control group, VCD-induced apoptosis in follicles increased the TUNEL-positive cell counts (p<0.001) with decreased number of follicles. On the other hand, DHEA significantly increased all three follicle types in the ovaries and decreased apoptosis (p<0.001). The decreased follicle number in all three follicle types after VCD treatment were found to be significantly increased after RES treatment (p<0.001). Apoptosis in the follicles was significantly decreased by RES administration (p<0.001). FSH values were found to be increased with VCD and to reach control values with DHEA and RES. E2 values significantly decreased with VCD, but significantly increased with RES and DHEA. Conclusion: Both DHEA and RES may improve VCD-induced diminished ovarian reserve. DHEA and RES increased the number of primary, primordial and growing follicles, with no significant difference between them.
DergiAraştırma MakalesiErişime Açık
  • 1. Sadrzadeh S, Verschuuren M, Schoonmade LJ, Lambalk CB, Painter RC. The effect of adverse intrauterine conditions, early childhood growth and famine exposure on age at menopause: a systematic review. J Dev Orig Health Dis 2017; 9: 127-36. [CrossRef]
  • 2. Atli M, Engin-Ustun Y, Tokmak A, Caydere M, Hucumenoglu S, Topcuoglu C. Dose dependent effect of resveratrol in preventing cisplatin-induced ovarian damage in rats: An experimental study. Reprod Biol 2017; 17: 274-80. [CrossRef]
  • 3. Zou, K, Yuan Z, Yang Z, et al. Production of offspring from a germline stem cell line derived from neonatal ovaries. Nat Cell Biol 2009; 11: 631-6. [CrossRef]
  • 4. Barad D, Gleicher N. Effect of dehydroepiandrosterone on oocyte and embryo yields, embryo grade and cell number in IVF. Hum Reprod 2006; 21: 2845-9. [CrossRef]
  • 5. Hassa H, Aydin Y, Ozatik O, Erol K, Ozatik Y. Effects of dehydroepiandrosterone (DHEA) on follicular dynamics in a diminished ovarian reserve in vivo model. Syst Biol Reprod Med 2015; 61: 117-21. [CrossRef]
  • 6. Ozatik FY, Erol K, Ozatik O. Estrogen modulating effects of resveratrol in female rats. Marmara Medical Journal 2016; 29: 95-101. [CrossRef]
  • 7. Özcan P, Fıçıcıoğlu C, Yıldırım ÖK, Özkan F, Akkaya H, Aslan İ. Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague-Dawley rats. Reprod Biomed Online 2015; 31: 404-10. [CrossRef]
  • 8. Ortega I, Duleba AJ. Ovarian actions of resveratrol. Ann N Y Acad Sci 2015; 1348: 86-96. [CrossRef]
  • 9. Hoyer P, Sipes G. Devolopment of Animal Model For Ovotoxicity Using 4-Vinylcyclohexene: A Case Study. Birth Defects Res B Dev Reprod Toxicol 2007; 80: 113-25. [CrossRef]
  • 10. Hu X, Christian P, Sipes IG, Hoyer PB. Expression and redistribution of cellular Bad, Bax, and Bcl-X(L) protein is associated with VCD-induced ovotoxicity in rats. Biol Reprod 2001; 65: 1489-95. [CrossRef]
  • 11. Lee JH, Lee M, Ahn C, Kang HY, Tran DN, Jeung EB. Parabens Accelerate Ovarian Dysfunction in a 4-Vinylcyclohexene Diepoxide-Induced Ovarian Failure Model. Int J Environ Res Public Health 2017; 8; 14(2). pii: E161. [CrossRef]
  • 12. Belgorosky D, Sander VA, Yorio MP, Faletti AG, Motta AB. Hyperandrogenism alters intraovarian parameters during early folliculogenesis in mice. Reprod Biomed Online 2010; 20: 797-807. [CrossRef]
  • 13. Hussein MR. Apoptosis in the ovary: Molecular mechanisms. Hum Reprod Update 2005; 11: 162-78. [CrossRef]
  • 14. Tilly JL. Ovarian follicle counts- not as simple as 1,2,3. Reprod. Biol. Endokriol 2003; 1: 11-3. [CrossRef]
  • 15. Hubner K, Fuhrmann G, Christenson LK, et al. Derivation of oocytes from Mouse embryonic stem cells. Science 2003; 300: 1251-6. [CrossRef]
  • 16. Morrison LJ, Marcinkiewicz JL. Tumor necrosis factor alpha enhances oocyte/follicle apoptosis in the neonatal rat ovary. Biol Reprod 2002; 66: 450-57. [CrossRef]
  • 17. Gougeon A. Regulation of Ovarian Follicular Development in Primates: Facts and Hypotheses. Endocr Rev 1996; 17: 121-55. [CrossRef]
  • 18. Rashtian J, Zhang J. Luteal-phase ovarian stimulation increases the number of mature oocytes in older women with severe diminished ovarian reserve. Syst Biol Reprod Med 2018; 22: 1-4. [CrossRef]
  • 19. Tsui KH, Lin LT, Wang PH. Luteal phase support with gonadotropin-releasing hormone agonist. J Chin Med Assoc 2014; 77: 505-7. [CrossRef]
  • 20. Gleicher N, Weghofer A, Barad DH. The role of androgens in follicle maturation and ovulation induction: friend or foe of infertility treatment? Reprod Biol Endocrinol 2011; 9: 116. [CrossRef]
  • 21. Nielsen ME, Rasmussen IA, Kristensen SG, et al. In human granulosa cells from small antral follicles, androgen receptor mRNA and androgen levels in follicular fluid correlate with FSH receptor mRNA. Mol Hum Reprod 2011; 17: 63-70. [CrossRef]
  • 22. Liu M, Yin Y, Ye X, et. al. Resveratrol protects against age-associated infertility in mice. Hum Reprod 2013; 28; 707-17. [CrossRef]
  • 23. Narkwichean A, Jayaprakasan K, Maalouf WE, Hernandez-Medrano JH, Pincott-Allen C, Campbell BK. Effects of dehydroepiandrosterone on in vivo ovine follicular development. Hum Reprod 2013; 29: 146-54. [CrossRef]
  • 24. Velez LM, Abruzzese GA, Heber MF, Ferreira SR, Motta AB. reatment with the synthetic PPARG ligand pioglitazone ameliorates early ovarian alterations induced by dehydroepiandrosterone in prepubertal rats. Pharmacol Rep 2019; 71: 96-104. [CrossRef]
  • 25. Mahmoud YI, Mahmoud AA, Abo-Zeid FS, Fares NH. Effects of dehydroepiandrosterone on the ovarian reserve and pregnancy outcomes in perimenopausal rats (DHEA and fertility in perimenopausal rats). Life Sci 2018; 199: 131-8. [CrossRef]
  • 26. Chen ZG, Luo LL, Xu JJ, Zhuang XL, Kong XX, Fu YC. Effects of plant polyphenols on ovarian follicular reserve in aging rats. Biochem Cell Biol 2010; 88: 737-45. [CrossRef]
  • 27. Morita Y, Wada-Hiraike O, Yano T, et al. Resveratrol promotes expression of SIRT1 and StAR in rat ovarian granulosa cells: an implicative role of SIRT1 in the ovary. Reprod Biol Endocrinol 2012; 10: 14. [CrossRef]
  • 28. Özatik FY, Özatik O, Yiğitaslan S, Ünel ÇÇ, Erol K. Protective role of resveratrol on testicular germ cells in mice with testicular toxicity. Turk J Urol 2017; 43: 444-50. [CrossRef]
  • 29. Lin X, Du J, Du Y, et al. Effects of dehydroepiandrosterone supplementation on mice with diminished ovarian reserve. Gynecol Endocrinol 2018; 34: 357-59. [CrossRef]
  • 30. Furat Rencber S, Kurnaz Ozbek S, Eraldemır C, et al. Effect of resveratrol and metformin on ovarian reserve and ultrastructure in PCOS: an experimental study. J Ovarian Res 2018; 11: 55. [CrossRef]
  • 31. Teixeira CJ, Ribeiro LM, Veras K, da Cunha Araujo LC, Curi R, de Oliveira Carvalho CR. Dehydroepiandrosterone supplementation is not beneficial in the late postmenopausal period in diet-induced obese rats. Life Sci 2018; 202: 110-6. [CrossRef]
  • 32. Mark-Kappeler CJ, Sen N, Keating AF, Sipes IG, Hoyer PB. Distribution and responsiveness of rat anti-Müllerian hormone during ovarian development and VCD-induced ovotoxicity. Toxicol Appl Pharmacol 2010; 15; 249: 1-7. [CrossRef]
  • 33. Baarends WM, Uilenbroek JT, Kramer P, et al. Anti-Müllerian hormone and anti-Müllerian hormone type II receptor messenger ribonucleic acid expression in rat ovaries during postnatal development, the estrous cycle, and gonadotropininduced follicle growth. Endocrinology 1995; 136; 4951-62. [CrossRef]
  • 34. Sahambi SK, Visser JA, Themmen AP, Mayer LP, Devine PJ. Correlation of serum anti-Müllerian hormone with accelerated follicle loss following 4-vinylcyclohexene diepoxide-induced follicle loss in mice. Reprod Toxicol 2008; 26: 116-22. [CrossRef]
  • 35. Tran DN, Jung EM, Yoo YM, et al. Depletion of follicles accelerated by combined exposure to phthalates and 4-vinylcyclohexene diepoxide, leading to premature ovarian failure in rats. Reprod Toxicol 2018; 80: 60-7. [CrossRef]
  • 36. Wong QHY, Yeung TWY, Yung SSF, Ko JKY, Li HWR, Ng EHY. The effect of 12-month dehydroepiandrosterone supplementation on the menstrual pattern, ovarian reserve markers, and safety profile in women with premature ovarian insufficiency. J Assist Reprod Genet 2018; 35: 857-62. [CrossRef]

TÜBİTAK ULAKBİM Ulusal Akademik Ağ ve Bilgi Merkezi Cahit Arf Bilgi Merkezi © 2019 Tüm Hakları Saklıdır.