Mojgan Noroozi KARIMABAD
(Rafsanjan University of Medical Sciences, Research Institute of Basic Medical Sciences, Molecular Medicine Research Center, Rafsanjan, Iran)
Sedigheh NIKNIA
(Rafsanjan University of Medical Sciences, Faculty of Medicine, Department of Clinical Biochemistry, Rafsanjan, Iran)
Mahdieh Bemani GOLNABADI
(Rafsanjan University of Medical Sciences, Faculty of Medicine, Department of Clinical Biochemistry, Rafsanjan, Iran)
Shirin Fattah POOR
(Rafsanjan University of Medical Sciences, Faculty of Medicine, Department of Clinical Biochemistry, Rafsanjan, Iran)
Mohammad Reza HAJIZADEH
(Rafsanjan University of Medical Sciences, Faculty of Medicine, Department of Clinical Biochemistry, Rafsanjan, Iran)
(Rafsanjan University of Medical Sciences, Research Institute of Basic Medical Sciences, Molecular Medicine Research Center, Rafsanjan, Iran)
Yıl: 2020Cilt: 52Sayı: 1ISSN: 1308-8734 / 1308-8742Sayfa Aralığı: 47 - 51İngilizce

14 0
Effect of Citrullus colocynthis Extract on Glycated Hemoglobin Formation (In Vitro)
Objective: Diabetes mellitus (DM) is typically a disorder of carbohydrate, fat, and protein metabolism. It develops due to a lack of or loss associated with insulin and/or resistance to insulin. Regarding complications of chemical substance use, drugs with few complications and high-reliability tannins are needed. This study aimed to determine the effect and mechanism of action of Citrullus colocynthis extract on the formation of glycated hemoglobin (HbA1c). Materials and Methods: A solution containing hemoglobin and glucose was incubated for 1, 2, 3, 4, 30, and 60 days by adding Citrullus colocynthis extract or glutathione. Quantitative measurement of HbA1c was performed using ion-exchange chromatography. Data were analyzed using ANOVA and two-way repeated measures test. A p<0.05 was considered statistically significant. Results: The Citrullus colocynthis extract in hyperglycemic conditions and with increasing time reduced the formation of HbA1c and thus inhibited the production of glycated proteins. By increasing the time and after initiation of reaction of extract concentrations (0, 0.1, 0.3, 0.5, and 1 g/dL), presently, there was a significant decrease in the formation of HbA1C compared to those in the control group (p<0.05). The decrease in glycation has been dose dependent. Conclusion: Therefore, Citrullus colocynthis could directly reduce the formation of HbA1c.
DergiAraştırma MakalesiErişime Açık
  • 1. Zinman B, Genuth S, Nathan DM. The diabetes control and complications trial/epidemiology of diabetes interventions and complications study: 30th anniversary presentations. Diabetes Care 2014; 37: 8. [CrossRef]
  • 2. Beagley J, Guariguata L, Weil C, Motala AA. Global estimates of undiagnosed diabetes in adults. Diabetes Res Clin Pract 2014; 103: 150- 60. [CrossRef]
  • 3. Butalia S, Kaplan GG, Khokhar B, Rabi DM. Environmental Risk Factors and Type 1 Diabetes: Past, Present, and Future. Can J Diabetes 2016; 40: 586-93. [CrossRef]
  • 4. Lee JH. Utility of the Glycemic Index in Practical Diabetes Management. Diabetes Metab J 2015; 16: 135-40. [CrossRef]
  • 5. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2014; 37: S81-90. [CrossRef]
  • 6. American Diabetes Association. Standards of medical care in diabetes-2015 abridged for primary care providers. Clinical Diabetes 2015; 33: 97-111. [CrossRef]
  • 7. Vlodavsky I, Iozzo RV, Sanderson RD. Heparanase: multiple functions in inflammation, diabetes and atherosclerosis. Matrix Biol 2013; 32: 220-2. [CrossRef]
  • 8. Xu W, Chen L, Shao R. Influence of glycation of plasma proteins in diabetes on the binding interaction with polyphenols. Curr Drug Metab 2014; 15: 116-9. [CrossRef]
  • 9. Singh VP, Bali A, Singh N, Jaggi AS. Advanced glycation end products and diabetic complications. Korean J Physiol Pharmacol 2014; 18: 1-14. [CrossRef]
  • 10. Adisakwattana S, Thilavech T, Chusak C. Mesona Chinensis Benth extract prevents AGE formation and protein oxidation against fructose-induced protein glycation in vitro. BMC Complement Altern Med 2014; 14: 130 [CrossRef]
  • 11. Singh R, Devi S, Gollen R. Role of free radical in atherosclerosis, diabetes and dyslipidaemia: larger‐than‐life. Diabetes Metab Res Rev 2015; 31: 113-26. [CrossRef]
  • 12. Hellwig M, Henle T. Baking, ageing, diabetes: a short history of the Maillard reaction. Angew Chem Int Ed Engl 2014; 53: 10316-29. [CrossRef]
  • 13. Oudegeest-Sander MH, Rikkert MGO, Smits P, et al. The effect of an advanced glycation endproduct crosslink breaker and exercise training on vascular function in older individuals: a randomized factorial design trial. Exp Gerontol 2013; 48: 1509-17. [CrossRef]
  • 14. Nagai R, Murray DB, Metz TO, Baynes JW. Chelation: a fundamental mechanism of action of AGE inhibitors, AGE breakers, and other inhibitors of diabetes complications. Diabetes 2012; 61: 549-59. [CrossRef]
  • 15. Matsuura N, Aradate T, Kurosaka C, et al. Potent protein glycation inhibition of plantagoside in Plantago major seeds. Biomed Res Int 2014; 2014: 208539. [CrossRef]
  • 16. Al-Rawi NH. Diabetes, Oxidative Stress, Antioxidants and Saliva: A Review: INTECH Open Access Publisher; 2012.
  • 17. Cazzola R, Cestaro B. Antioxidant Spices and Herbs Used in Diabetes-Chapter 9. 2014. [CrossRef]
  • 18. Agati G, Azzarello E, Pollastri S, Tattini M. Flavonoids as antioxidants in plants: location and functional significance. Plant Sci 2012; 196: 67-76. [CrossRef]
  • 19. Kaewnarin K, Niamsup H, Shank L, Rakariyatham N. Antioxidant and antiglycation activities of some edible and medicinal plants. Chiang Mai J Sci 2014; 41: 105-16.
  • 20. Mahmoodi M, Hosseini J, Hosseini-zijoud S, Pooladvand V, Asadpour M, Eghbali H. The Effect of Citrullus Colocynthis Hydroalcoholic Extract on in vitro Albumin Glycation. JRUMS 2013; 12: 3-14. [CrossRef]
  • 21. Lakshmi B, Sendrayaperumal V, Subramanian S. Beneficial Effects of Citrullus Colocynthis Seeds Extract Studied in Alloxan-induced Diabetic Rats. Int J Pharm Sci 2013; 19: 47-55.
  • 22. Shi C, Karim S, Wang C, Zhao M, Murtaza G. A review on antidiabetic activity of Citrullus colocynthis Schrad. Acta Pol Pharm 2014; 71: 363-7.
  • 23. Ghafouri-Khosrowshahi A, Farzami B, Mohammadi-Bardbori A. the inhibitory effect of garlic extract on formation of glycated hemoglobin and AGEPs. J Med Sci 2007; 7: 1039-43. [CrossRef]
  • 24. Turk Z, Mesic R, Benko B. Comparison of advanced glycation endproducts on haemoglobin (Hb-AGE) and haemoglobin A1c for the assessment of diabetic control. Clinica Chimica Acta 1998; 277: 159-70. [CrossRef]
  • 25. Jing Z, Kuang L, Wang Y, et al. ADMA: a specific biomarker for pathologic progress in diabetic microvascular complications? Biomark Med 2016; 10: 385-95. [CrossRef]
  • 26. Gan T, Liao B, Xu G. The clinical usefulness of glycated albumin in patients with diabetes and chronic kidney disease: Progress and challenges. J Diabetes Complications 2018; 32: 876-84. [CrossRef]
  • 27. Rendell M, Nierenberg J, Brannan C, et al. Inhibition of glycation of albumin and hemoglobin by acetylation in vitro and in vivo. J Lab Clin Med 1986; 108: 286-93.
  • 28. Chevion S, Hofmann M, Ziegler R, Chevion M, Nawroth PP. The antioxidant properties of thioctic acid: characterization by cyclic voltammetry. Biochem Mol Biol Int 1997; 41: 317-27. [CrossRef]
  • 29. Lal S, Chithra P, Chandrakasan G. The possible relevance of autoxidative glycosylation in glucose mediated alterations of proteins: an in vitro study on myofibrillar proteins. Mol Cell Biochem 1996; 154: 95-100. [CrossRef]
  • 30. Slight SH, Feather MS, Ortwerth BJ. Glycation of lens proteins by the oxidation products of ascorbic acid. Biochimica et Biophysica Acta 1990; 1038: 367-74. [CrossRef]
  • 31. Ceriello A, Giugliano D, Quatraro A, Dello Russo P, Torella R. A preliminary note on inhibiting effect of alpha-tocopherol (vit. E) on protein glycation. Diabetes Metab 1988; 14: 40-2.
  • 32. Jain SK, McVie R, Jaramillo JJ, Palmer M, Smith T. Effect of modest vitamin E supplementation on blood glycated hemoglobin and triglyceride levels and red cell indices in type I diabetic patients. J Am Coll Nutr 1996; 15: 458-61. [CrossRef]
  • 33. Shaikh J, Shaikh D, Rahman AB, Shafi S. Antimicrobial and toxicological studies on fruit pulp of Citrullus colocynthis L. Pak J Pharm Sci 2016; 29: 9-15.
  • 34. Rizvi M, Saeed A, Zubairy N. Medicinal plants history, cultivation and uses. Karachi: Hamdard Institut Adv Studies Res 2007: 85-7.
  • 35. Kumar S, Kumar D, SAROHA K, Singh N, Vashishta B. Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract. Acta Pharmaceutica 2008; 58: 215-20. [CrossRef]
  • 36. Usmanghani K, Saeed A, Alam MT. Indusyunic medicine: traditional medicine of herbal, animal, and mineral origin in Pakistan: Dept. of Pharmacognosy, Faculty of Pharmacy, University of Karachi; 1997.

TÜBİTAK ULAKBİM Ulusal Akademik Ağ ve Bilgi Merkezi Cahit Arf Bilgi Merkezi © 2019 Tüm Hakları Saklıdır.