Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae)

jahanjou, farshid; GHOLAMZADEH CHITGAR, Moloud; GHADAMYARI, Mohammad; Hosseini, Reza

Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae)

Moloud GHOLAMZADEH CHITGAR, (Education and Extension Organization (AREEO), Mazandaran Agricultural and Natural Resources Research and Education Center, Agricultural Research, Plant Protection Research Department, Sari, Iran)

Farshid JAHANJOU, (University of Guilan, Faculty of Agricultural Science, Associate Professors of Department of Plant Protection, Rasht, Iran)

Mohammad GHADAMYARI, (University of Guilan, Faculty of Agricultural Science, Associate Professors of Department of Plant Protection, Rasht, Iran)

Reza HOSSEINI (University of Guilan, Faculty of Agricultural Science, Associate Professors of Department of Plant Protection, Rasht, Iran)

Journal of the Entomological Research Society

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Yıl: 2019 Cilt: 21 Sayı: 3 Sayfa Aralığı: 271 - 280 Metin Dili: İngilizce İndeks Tarihi: 26-06-2020

Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae)

Öz:

Knowledge on digestive proteases enzymes of insects needed for making plant expressing proteaseinhibitors to reach an alternative method to chemical control. In present study, biochemical properties ofdigestive proteases were determined in the alimentary canal of the small black and yellow wasp, Allantusviennensis Schr. (Hym.: Tenthredinidae) as important pest of Rose bushes. Larvae of A. viennensis werecollected from rose plants in Rasht, Guilan province of Iran in summer (2016). Determining the proteolyticactivity in gut of different larval instar of A. viennensis (2-5) showed that the enzyme activity increasedwith growing the larvae. The higher activity was found in the fifth instars larvae (7.46±0.06 µmol-1min-1mg-1protein). Also, comparison of proteolytic activities in different parts of digestive system of the fifth instarslarvae showed that the enzyme activity in midgut was higher than that found in the foregut and hindgut. Theoptimal pH and temperature for enzyme activity in gut of fifth instars larvae were found at pH 10 and 30°C,respectively. Most inhibitory effect on the protease activity was obtained by PMSF as serine proteinasesinhibitor (36.85%). The results of SDS-PAGE confirm the obtained data of inhibition assay. It showed thatthe serine proteinases are the major hydrolysing enzymes in the gut of larvae of A. viennensis.

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APA	GHOLAMZADEH CHITGAR M, jahanjou f, GHADAMYARI M, Hosseini R (2019). Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae). , 271 - 280.
Chicago	GHOLAMZADEH CHITGAR Moloud,jahanjou farshid,GHADAMYARI Mohammad,Hosseini Reza Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae). (2019): 271 - 280.
MLA	GHOLAMZADEH CHITGAR Moloud,jahanjou farshid,GHADAMYARI Mohammad,Hosseini Reza Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae). , 2019, ss.271 - 280.
AMA	GHOLAMZADEH CHITGAR M,jahanjou f,GHADAMYARI M,Hosseini R Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae). . 2019; 271 - 280.
Vancouver	GHOLAMZADEH CHITGAR M,jahanjou f,GHADAMYARI M,Hosseini R Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae). . 2019; 271 - 280.
IEEE	GHOLAMZADEH CHITGAR M,jahanjou f,GHADAMYARI M,Hosseini R "Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae)." , ss.271 - 280, 2019.
ISNAD	GHOLAMZADEH CHITGAR, Moloud vd. "Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae)". (2019), 271-280.

APA	GHOLAMZADEH CHITGAR M, jahanjou f, GHADAMYARI M, Hosseini R (2019). Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae). Journal of the Entomological Research Society, 21(3), 271 - 280.
Chicago	GHOLAMZADEH CHITGAR Moloud,jahanjou farshid,GHADAMYARI Mohammad,Hosseini Reza Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae). Journal of the Entomological Research Society 21, no.3 (2019): 271 - 280.
MLA	GHOLAMZADEH CHITGAR Moloud,jahanjou farshid,GHADAMYARI Mohammad,Hosseini Reza Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae). Journal of the Entomological Research Society, vol.21, no.3, 2019, ss.271 - 280.
AMA	GHOLAMZADEH CHITGAR M,jahanjou f,GHADAMYARI M,Hosseini R Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae). Journal of the Entomological Research Society. 2019; 21(3): 271 - 280.
Vancouver	GHOLAMZADEH CHITGAR M,jahanjou f,GHADAMYARI M,Hosseini R Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae). Journal of the Entomological Research Society. 2019; 21(3): 271 - 280.
IEEE	GHOLAMZADEH CHITGAR M,jahanjou f,GHADAMYARI M,Hosseini R "Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae)." Journal of the Entomological Research Society, 21, ss.271 - 280, 2019.
ISNAD	GHOLAMZADEH CHITGAR, Moloud vd. "Biochemical Characterization of the Digestive Proteases in the Small Black and Yellow Wasp, Allantus viennensis Schr. (Hym.: Tenthredinidae)". Journal of the Entomological Research Society 21/3 (2019), 271-280.