Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis

Filiz YEŞİLIRMAK, (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Kimya Bölümü, İzmir, Türkiye)
Tuğrul DORUK, (Gebze Teknik Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Kocaeli, Türkiye)
Şerif YILMAZ, (Gebze Teknik Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Kocaeli, Türkiye)
Sedef TUNCA GEDİK (Gebze Teknik Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Kocaeli, Türkiye)
Turkish Journal of Biology
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Yıl: 2018 Cilt: 42 Sayı: 1 Sayfa Aralığı: 87 - 102 Metin Dili: İngilizce DOI: 10.3906/biy-1711-9 İndeks Tarihi: 09-07-2020

Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis

Öz:
Polyphosphate polymer (polyP) plays a very important role in every living cell. Synthesis of this linear polymer of phosphate(Pi) residues is catalyzed by the polyphosphate kinase (PPK) enzyme. It was shown that high levels of intracellular polyphosphatestimulated endotoxin production by Bacillus thuringiensis subsp. israelensis (Bti). In this study, proteomic analysis of the wild-typeand two mutant strains, overexpressing the ppk gene (Bti pHTppk) and without the ppk gene (Bti ∆ppk), were used to clarify therelation between polyP and endotoxin production. Intracellular proteins were separated by two-dimensional gel electrophoresis; 41spots of interest (proteins differentially expressed) were obtained and 35 of them were identified by mass spectrometry. Analysis of theprotein profiles showed that there is a general decrease in the expression levels of proteins related with energy metabolism, amino acidmetabolism, and purine biosynthesis in both Bti pHTppk and Bti ∆ppk. Gluconeogenesis and fatty acid metabolism were also sloweddown in both strains, whereas expression of stress response proteins increased compared to the wild-type. These results suggested thatchanges in polyP concentration cause a general stress condition inside the cell, which in turn stimulates secondary metabolite synthesis.
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APA YEŞİLIRMAK F, DORUK T, YILMAZ Ş, Tunca Gedik S (2018). Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis. , 87 - 102. 10.3906/biy-1711-9
Chicago YEŞİLIRMAK Filiz,DORUK Tuğrul,YILMAZ Şerif,Tunca Gedik Sedef Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis. (2018): 87 - 102. 10.3906/biy-1711-9
MLA YEŞİLIRMAK Filiz,DORUK Tuğrul,YILMAZ Şerif,Tunca Gedik Sedef Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis. , 2018, ss.87 - 102. 10.3906/biy-1711-9
AMA YEŞİLIRMAK F,DORUK T,YILMAZ Ş,Tunca Gedik S Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis. . 2018; 87 - 102. 10.3906/biy-1711-9
Vancouver YEŞİLIRMAK F,DORUK T,YILMAZ Ş,Tunca Gedik S Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis. . 2018; 87 - 102. 10.3906/biy-1711-9
IEEE YEŞİLIRMAK F,DORUK T,YILMAZ Ş,Tunca Gedik S "Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis." , ss.87 - 102, 2018. 10.3906/biy-1711-9
ISNAD YEŞİLIRMAK, Filiz vd. "Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis". (2018), 87-102. https://doi.org/10.3906/biy-1711-9
APA YEŞİLIRMAK F, DORUK T, YILMAZ Ş, Tunca Gedik S (2018). Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis. Turkish Journal of Biology, 42(1), 87 - 102. 10.3906/biy-1711-9
Chicago YEŞİLIRMAK Filiz,DORUK Tuğrul,YILMAZ Şerif,Tunca Gedik Sedef Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis. Turkish Journal of Biology 42, no.1 (2018): 87 - 102. 10.3906/biy-1711-9
MLA YEŞİLIRMAK Filiz,DORUK Tuğrul,YILMAZ Şerif,Tunca Gedik Sedef Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis. Turkish Journal of Biology, vol.42, no.1, 2018, ss.87 - 102. 10.3906/biy-1711-9
AMA YEŞİLIRMAK F,DORUK T,YILMAZ Ş,Tunca Gedik S Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis. Turkish Journal of Biology. 2018; 42(1): 87 - 102. 10.3906/biy-1711-9
Vancouver YEŞİLIRMAK F,DORUK T,YILMAZ Ş,Tunca Gedik S Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis. Turkish Journal of Biology. 2018; 42(1): 87 - 102. 10.3906/biy-1711-9
IEEE YEŞİLIRMAK F,DORUK T,YILMAZ Ş,Tunca Gedik S "Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis." Turkish Journal of Biology, 42, ss.87 - 102, 2018. 10.3906/biy-1711-9
ISNAD YEŞİLIRMAK, Filiz vd. "Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis". Turkish Journal of Biology 42/1 (2018), 87-102. https://doi.org/10.3906/biy-1711-9
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17-04-2024 15:2

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