Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

YANIKOĞLU, Semra; ÖZTÜRK, Leyla; ZARARSIZ, Duran; BİLGİN, Burak; Kızık, Sezgin; TURGUT, İlhan

Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

Duran ZARARSIZ, (Agromar A.Ş. Tohumculuk Şirketi, Karacabey, Bursa, Türkiye)

Leyla ÖZTÜRK, (Agromar A.Ş. Tohumculuk Şirketi, Karacabey, Bursa, Türkiye)

Semra Yanıkoğlu, (Agromar A.Ş. Tohumculuk Şirketi, Karacabey, Bursa, Türkiye)

İlhan TURGUT, (Uludağ Üniversitesi, Ziraat Fakültesi, Tarla Ürünleri Bölümü, Bursa, Türkiye)

Sezgin KIZIK, (Agromar A.Ş. Tohumculuk Şirketi, Karacabey, Bursa, Türkiye)

Burak BİLGİN (Agromar A.Ş. Tohumculuk Şirketi, Karacabey, Bursa, Türkiye)

Tarım Bilimleri Dergisi

7 3

Yıl: 2019 Cilt: 25 Sayı: 1 Sayfa Aralığı: 62 - 69 Metin Dili: İngilizce İndeks Tarihi: 17-06-2020

Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn

Öz:

This research was conducted at the breeding station of the Turkish breeding company Agromar A.Ş in the city of Bursain Turkey during the 2013 and 2014 growing seasons. Used from within the same heterotic group crossings, 7 donormaterials were obtained during the 2012 winter season in the greenhouse. The inducer line RWK-76xRWS, providedby University of Hohenheim, Germany, was used for generating haploid seeds. The donor and inducer crossing wasperformed during the 2013 summer season. The haploid selection and chromosome doubling were performed duringthe 2014 summer season. Seven donors were used for haploid induction which name are DNR1, DNR2, DNR3,DNR4, DNR5, DNR6, DNR7 respectively, from each donor diffrent amount of ear crosses were performed (DNR1:16ears, DNR2:10 ears, DNR3:10 ears, DNR4:12 ears, DNR5:11 ears, DNR6:13 ears, DNR7:11 ears). According to thepresent study, the average induction rate found ranged from 7.1 to 12.8%, and the average seedling survival rate in thegreenhouse after colchicine application ranged from 57.9 to 77.6%. After transplanting to the fild, 78.3-92.6% of theseplants survived. As a result of this research, the chromosome doubling rate ranged from 22.5 to 48.3% depending onthe donor material. These result indicates that maternal haploid selection visually is easy. Haploid induction rate (HIR)changes from donor to donor, its mean genotype and environment is effctive for HIR. Average chromosome doublingrate is lower than other researchers’ results, it is also effcted by genotype and chromosome doubling methods.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ZARARSIZ D, ÖZTÜRK L, YANIKOĞLU S, TURGUT İ, Kızık S, BİLGİN B (2019). Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn. , 62 - 69.
Chicago	ZARARSIZ Duran,ÖZTÜRK Leyla,YANIKOĞLU Semra,TURGUT İlhan,Kızık Sezgin,BİLGİN Burak Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn. (2019): 62 - 69.
MLA	ZARARSIZ Duran,ÖZTÜRK Leyla,YANIKOĞLU Semra,TURGUT İlhan,Kızık Sezgin,BİLGİN Burak Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn. , 2019, ss.62 - 69.
AMA	ZARARSIZ D,ÖZTÜRK L,YANIKOĞLU S,TURGUT İ,Kızık S,BİLGİN B Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn. . 2019; 62 - 69.
Vancouver	ZARARSIZ D,ÖZTÜRK L,YANIKOĞLU S,TURGUT İ,Kızık S,BİLGİN B Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn. . 2019; 62 - 69.
IEEE	ZARARSIZ D,ÖZTÜRK L,YANIKOĞLU S,TURGUT İ,Kızık S,BİLGİN B "Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn." , ss.62 - 69, 2019.
ISNAD	ZARARSIZ, Duran vd. "Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn". (2019), 62-69.

APA	ZARARSIZ D, ÖZTÜRK L, YANIKOĞLU S, TURGUT İ, Kızık S, BİLGİN B (2019). Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn. Tarım Bilimleri Dergisi, 25(1), 62 - 69.
Chicago	ZARARSIZ Duran,ÖZTÜRK Leyla,YANIKOĞLU Semra,TURGUT İlhan,Kızık Sezgin,BİLGİN Burak Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn. Tarım Bilimleri Dergisi 25, no.1 (2019): 62 - 69.
MLA	ZARARSIZ Duran,ÖZTÜRK Leyla,YANIKOĞLU Semra,TURGUT İlhan,Kızık Sezgin,BİLGİN Burak Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn. Tarım Bilimleri Dergisi, vol.25, no.1, 2019, ss.62 - 69.
AMA	ZARARSIZ D,ÖZTÜRK L,YANIKOĞLU S,TURGUT İ,Kızık S,BİLGİN B Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn. Tarım Bilimleri Dergisi. 2019; 25(1): 62 - 69.
Vancouver	ZARARSIZ D,ÖZTÜRK L,YANIKOĞLU S,TURGUT İ,Kızık S,BİLGİN B Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn. Tarım Bilimleri Dergisi. 2019; 25(1): 62 - 69.
IEEE	ZARARSIZ D,ÖZTÜRK L,YANIKOĞLU S,TURGUT İ,Kızık S,BİLGİN B "Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn." Tarım Bilimleri Dergisi, 25, ss.62 - 69, 2019.
ISNAD	ZARARSIZ, Duran vd. "Production of Double Haploid Plants Using In Vivo Haploid Techniques in Corn". Tarım Bilimleri Dergisi 25/1 (2019), 62-69.