Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status

Bayrak, Serdar; Aktas, Safiye; ALTUN, Zekiye; Olgun, Nur; INCE, Dilek

doi:10.4999/uhod.193154

Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status

Serdar BAYRAK, (Dokuz Eylül Üniversitesi, Onkoloji Enstitüsü, Klinik Onkoloji Anabilim Dalı, İzmir, Türkiye)

Zekiye ALTUN, (Dokuz Eylül Üniversitesi, Onkoloji Enstitüsü, Temel Onkoloji Anabilim Dalı, İzmir, Türkiye)

DİLEK İNCE, (Dokuz Eylül Üniversitesi, Onkoloji Enstitüsü, Çocuk Onkolojisi Anabilim Dalı, İzmir, Türkiye)

Safiye AKTAŞ, (Dokuz Eylül Üniversitesi, Onkoloji Enstitüsü, Temel Onkoloji Anabilim Dalı, İzmir, Türkiye)

Nur OLGUN (Dokuz Eylül Üniversitesi, Onkoloji Enstitüsü, Çocuk Onkolojisi Anabilim Dalı, İzmir, Türkiye)

Uluslararası Hematoloji-Onkoloji Dergisi

4 1

Yıl: 2019 Cilt: 29 Sayı: 2 Sayfa Aralığı: 70 - 78 Metin Dili: İngilizce DOI: 10.4999/uhod.193154 İndeks Tarihi: 19-06-2020

Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status

Öz:

Betulinic acid-(BA) has apoptotic effects on neuroblastoma. This study explored the effects of cisplatin-(CP), BA and combination ofBA and CP (BA-CP) on cell proliferation, apoptotic cell death and expressions of metastasis related genes in neuroblastoma. In thisstudy, N-MYC positive and negative neuroblastoma cell lines, KELLY and SHSY5Y, were treated with BA, CP and BA-CP combinations. BA-CP combinations showed synergistic activity on cell growth inhibition and apoptotic cell death in both neuroblastoma celllines. In SHSY5Y cells BA, CP or BA-CP treatments induced downregulation most of the metastatic genes’ expressions. In KELLYcells, while CP treatment decreased some metastatic gene expressions, BA or BA-CP treatment reduced only DENR and RB1. BAand BA-CP showed prominent effect on metastasis related gene expressions in predominantly KELLY cells. The results of this studysuggest that BA may be a promising anti-cancer agent for neuroblastoma treatment, whereas CP is still the most suitable chemotherapeutic drug for aggressive neuroblastoma.

Anahtar Kelime:

Betulinik Asit ve Sisplatin Nöroblastom Hücrelerinde N-MYC Durumlarına Göre Metastaz iİişkili Genleri İnhibe Eder

Öz:

Betulinik asid-(BA), nöroblastom üzerinde apoptotik etkiye sahiptir. Bu çalışma sisplatin-(CP), BA ve BA ile CP kombinasyonunun (BACP) nöroblastomda hücre çoğalması, apoptotik hücre ölümü ve metastaz ilişkli gen ekspresyonları üzerindeki etkilerini araştırmıştır. Bu çalışmada, N-MYC pozitif ve negatif, KELLY ve SHSY5Y, nöroblastom hücrelerine BA, CP ve kombinasyonları uygulanmıştır. BA-CP kombinasyonu her iki nöroblastom hücre hattında hücre gelişimi inhibisyonu ve apoptotik hücre ölümü üzerine sinerjik aktivite göstermiştir. SHSY5Y hücrelerinde BA, CP veya BA-CP uygulamaları, metastatik gen ekspresyonlarının çoğunun downregülasyonunu indüklemiştir. KELLY hücrelerinde CP uygulaması bazı metastatik gen ekspresyonlarını azaltırken, BA ve ya BA-CP kombinasyonları metastaza bağlı gen ekspresyonları üzerinde belirgin bir etki göstermiştir. Bu çalışma sonuçları, BA’nın neuroblastom tedavisi için umut verici bir anti-kanser ajan olabileceğini önermekle birlikte, CP halen özellikle agresif nöroblastom için en uygun kemoterapötik ilaçtır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Bayrak S, ALTUN Z, INCE D, Aktas S, Olgun N (2019). Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status. , 70 - 78. 10.4999/uhod.193154
Chicago	Bayrak Serdar,ALTUN Zekiye,INCE Dilek,Aktas Safiye,Olgun Nur Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status. (2019): 70 - 78. 10.4999/uhod.193154
MLA	Bayrak Serdar,ALTUN Zekiye,INCE Dilek,Aktas Safiye,Olgun Nur Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status. , 2019, ss.70 - 78. 10.4999/uhod.193154
AMA	Bayrak S,ALTUN Z,INCE D,Aktas S,Olgun N Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status. . 2019; 70 - 78. 10.4999/uhod.193154
Vancouver	Bayrak S,ALTUN Z,INCE D,Aktas S,Olgun N Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status. . 2019; 70 - 78. 10.4999/uhod.193154
IEEE	Bayrak S,ALTUN Z,INCE D,Aktas S,Olgun N "Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status." , ss.70 - 78, 2019. 10.4999/uhod.193154
ISNAD	Bayrak, Serdar vd. "Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status". (2019), 70-78. https://doi.org/10.4999/uhod.193154

APA	Bayrak S, ALTUN Z, INCE D, Aktas S, Olgun N (2019). Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status. Uluslararası Hematoloji-Onkoloji Dergisi, 29(2), 70 - 78. 10.4999/uhod.193154
Chicago	Bayrak Serdar,ALTUN Zekiye,INCE Dilek,Aktas Safiye,Olgun Nur Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status. Uluslararası Hematoloji-Onkoloji Dergisi 29, no.2 (2019): 70 - 78. 10.4999/uhod.193154
MLA	Bayrak Serdar,ALTUN Zekiye,INCE Dilek,Aktas Safiye,Olgun Nur Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status. Uluslararası Hematoloji-Onkoloji Dergisi, vol.29, no.2, 2019, ss.70 - 78. 10.4999/uhod.193154
AMA	Bayrak S,ALTUN Z,INCE D,Aktas S,Olgun N Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status. Uluslararası Hematoloji-Onkoloji Dergisi. 2019; 29(2): 70 - 78. 10.4999/uhod.193154
Vancouver	Bayrak S,ALTUN Z,INCE D,Aktas S,Olgun N Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status. Uluslararası Hematoloji-Onkoloji Dergisi. 2019; 29(2): 70 - 78. 10.4999/uhod.193154
IEEE	Bayrak S,ALTUN Z,INCE D,Aktas S,Olgun N "Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status." Uluslararası Hematoloji-Onkoloji Dergisi, 29, ss.70 - 78, 2019. 10.4999/uhod.193154
ISNAD	Bayrak, Serdar vd. "Betulinic Acid and Cisplatin Inhibit Metastasis Related Genes in Neuroblastoma Cells Due to Their N-MYC Status". Uluslararası Hematoloji-Onkoloji Dergisi 29/2 (2019), 70-78. https://doi.org/10.4999/uhod.193154