Objective: The aim of study was to investigate the perception of pain associated withrenal biopsy with visual analog scale (VAS), and the factors that might influence pain perception.Material and Methods: A total of 100 patients who had undergone native kidney biopsy in our hospital between May 2014-November 2016 were enrolled. Patients were not receiving analgesics,sedatives, nonsteroidal anti-inflammatory drugs or antihistamines at the time of biopsy. Renalbiopsy was performed under ultrasound guidance following local anesthesia. Pain was rated by VAS(1-10) when anesthetic agent was injected (VAS-1), when biopsy was performed (VAS-2), and 30minutes after biopsy (VAS-3). Associations of clinical, laboratory, and radiological data with painscores were analysed. Results: A total of 100 patients undergoing renal biopsy were enrolled. VAS2 was found significantly higher than VAS-1, and VAS-3 scores. Patient age, past surgical history,serum creatinine, biopsy side, vertical length and paranchimal thickness of the kidney in whichbiopsy was performed, needle size, number of cores taken were not associated with VAS 1-2-3scores. Proteinuria (≥3.5 g/dL) was found associated with VAS scores. Conclusion: Perception ofpain associated with renal biopsy was independent from many clinical and anatomical factors, andwas difficult to be predicted.