Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt

ASSAR, Samy A EL; SHREADAH, Mohamed A.; MONEAM, Nehad M Abd EL; ADAM, Asmaa NABIL

doi:10.4274/tjps.galenos.2018.72677

Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt

Asmaa NABIL ADAM, (Marine Biotechnology and Natural Products Lab (MBNP), National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt)

Mohamed A. SHREADAH, (Marine Biotechnology and Natural Products Lab (MBNP), National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt)

Nehad M Abd EL MONEAM, (Alexandria University, Faculty of Science, Department of Biochemistry, Alexandria, Egypt)

Samy A EL ASSAR (Alexandria University, Faculty of Science, Department of Botany and Microbiology, Alexandria, Egypt)

Turkish Journal of Pharmaceutical Sciences

1 0

Yıl: 2020 Cilt: 17 Sayı: 2 Sayfa Aralığı: 127 - 135 Metin Dili: İngilizce DOI: 10.4274/tjps.galenos.2018.72677 İndeks Tarihi: 27-11-2020

Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt

Öz:

Objectives: The present study revealed the presence of bioactive constituents in Hyrtios aff. erectus sponge (HES) extract collected from the RedSea using skin and scuba diving.Materials and Methods: Cytotoxicity was tested against hepatocellular carcinoma cell lines as a prescreening test.Results: The HES extract had high contents of total phenolic compounds (0.061 mg/g), flavonoids (0.2839 mg/g), and carotenoids (1.976 mg/g).Moreover, the HES extract showed high antioxidant capacity with 93.0% and 99% at 1 mg using 2.2’-Diphenyl-α-picrylhydrazyl and 2.2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), respectively. Cytotoxic activity against cancerous cell lines showed that the HES extract could inhibit cellgrowth effectively with IC50=47.5 µg/mL. Furthermore, anticancer activity using protein tyrosine kinase and sphingosine kinase 1 inhibitor screeningassays resulted in 71.66% and 85.21% inhibition activity, respectively. The anti-inflammatory assays showed that the inhibition activity againstcyclooxygenase (COX1), COX2, interleukin-6, and tumor necrosis factor-α was 71.82%, 81.13%, 80.89%, and 59.74%, respectively. At the same time,the anti-Alzheimer results using acetylcholine inhibition assay showed high activity at 1 mg with 83.51%. Additionally, the antiviral activity using thereverse transcriptase inhibition assay was 91.70%.Conclusion: This marine sponge isolated from the Red Sea showed tremendous activity against many diseases and it is considered an excellentsource for bioactive pharmaceutical compounds.

Anahtar Kelime:

Mısır’ın Kızıl Deniz Kıyısındaki Sünger Hyrtios aff. Erectus’tan İzole Edilen Farklı Sekonder Metabolitlerin Biyoaktiviteleri

Öz:

Amaç: Bu çalışma normal ve tüplü dalışlar ile Kızıl Deniz’den toplanan Hyrtios aff. erectus süngerindeki (HES) biyoaktif bileşiklerin varlığını göstermeyi amaçlamıştır. Gereç ve Yöntemler: Sitotoksisite hepatosellüler karsinoma hücre hatlarında ön izleme testleriyle belirlenmiştir. Bulgular: HES ekstresi yüksek derecede fenolik bileşikler, (0,061 mg/g), flavonoidler (0,2839 mg/g) ve karotenoidleri (1,976 mg/g) içermektedir. Ayrıca, 1 mg HES ekstresi 2’-difenil-α-pikrilhidrazil ve 2,2’-azino-bis (3-etilbenzotiyazolin-6-sulfonik asit) ile sırasıyla %93,0 ve %99 ile yüksek antioksidan kapasite göstermiştir. HES ekstresi kanseröz hücre hatlarına karşı sitotoksik bulunmuştur ve hücre büyümesini inhibe edebilmektedir (IC50=47,5 µg/mL). Dahası, protein tirozin kinaz ve sfingosin kinaz 1 inhibitör izleme testleri kullanılarak belirlenen antikanser aktivitesi, sırasıyla %71,66 ve %85,21 inhibitör aktiviteyle sonuçlanmıştır. Antienflamatuvar testler siklooksijenaz 1 (COX1 ), siklooksijenaz 2 (COX2 ), interlökin-6 ve tümör nekroz faktör-α inhibitor aktivitesinin sırasıyla %71,82, %81,13, %80,89 ve %59,74 olduğunu göstermiştir. Aynı zamanda, asetil kolin esteraz inhibisyon teti kıllanılarak elde edilen anti-Alzheimer sonuçlar 1 mg dozda yüksek aktiviteyi (%83,51) belirlemiştir. Ek olarak, geri transkriptaz inhibisyon yöntemi kullanılarak bakılan antiviral aktivitesi %91,70’dir.Sonuç: Kızıl Deniz’den elde edilen bu deniz süngeri birçok hastalığa karşı büyük bir aktivite göstermiştir ve biyoaktif farmasötik bileşikler için mükemmel bir kaynak kabul edilebilir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ADAM A, SHREADAH M, MONEAM N, ASSAR S (2020). Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt. , 127 - 135. 10.4274/tjps.galenos.2018.72677
Chicago	ADAM Asmaa NABIL,SHREADAH Mohamed A.,MONEAM Nehad M Abd EL,ASSAR Samy A EL Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt. (2020): 127 - 135. 10.4274/tjps.galenos.2018.72677
MLA	ADAM Asmaa NABIL,SHREADAH Mohamed A.,MONEAM Nehad M Abd EL,ASSAR Samy A EL Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt. , 2020, ss.127 - 135. 10.4274/tjps.galenos.2018.72677
AMA	ADAM A,SHREADAH M,MONEAM N,ASSAR S Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt. . 2020; 127 - 135. 10.4274/tjps.galenos.2018.72677
Vancouver	ADAM A,SHREADAH M,MONEAM N,ASSAR S Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt. . 2020; 127 - 135. 10.4274/tjps.galenos.2018.72677
IEEE	ADAM A,SHREADAH M,MONEAM N,ASSAR S "Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt." , ss.127 - 135, 2020. 10.4274/tjps.galenos.2018.72677
ISNAD	ADAM, Asmaa NABIL vd. "Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt". (2020), 127-135. https://doi.org/10.4274/tjps.galenos.2018.72677

APA	ADAM A, SHREADAH M, MONEAM N, ASSAR S (2020). Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt. Turkish Journal of Pharmaceutical Sciences, 17(2), 127 - 135. 10.4274/tjps.galenos.2018.72677
Chicago	ADAM Asmaa NABIL,SHREADAH Mohamed A.,MONEAM Nehad M Abd EL,ASSAR Samy A EL Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt. Turkish Journal of Pharmaceutical Sciences 17, no.2 (2020): 127 - 135. 10.4274/tjps.galenos.2018.72677
MLA	ADAM Asmaa NABIL,SHREADAH Mohamed A.,MONEAM Nehad M Abd EL,ASSAR Samy A EL Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt. Turkish Journal of Pharmaceutical Sciences, vol.17, no.2, 2020, ss.127 - 135. 10.4274/tjps.galenos.2018.72677
AMA	ADAM A,SHREADAH M,MONEAM N,ASSAR S Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt. Turkish Journal of Pharmaceutical Sciences. 2020; 17(2): 127 - 135. 10.4274/tjps.galenos.2018.72677
Vancouver	ADAM A,SHREADAH M,MONEAM N,ASSAR S Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt. Turkish Journal of Pharmaceutical Sciences. 2020; 17(2): 127 - 135. 10.4274/tjps.galenos.2018.72677
IEEE	ADAM A,SHREADAH M,MONEAM N,ASSAR S "Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt." Turkish Journal of Pharmaceutical Sciences, 17, ss.127 - 135, 2020. 10.4274/tjps.galenos.2018.72677
ISNAD	ADAM, Asmaa NABIL vd. "Various In Vitro Bioactivities of Secondary Metabolites Isolated from the Sponge Hyrtios aff. Erectus from the Red Sea Coast of Egypt". Turkish Journal of Pharmaceutical Sciences 17/2 (2020), 127-135. https://doi.org/10.4274/tjps.galenos.2018.72677