Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?
Yıl: 2019 Cilt: 29 Sayı: 4 Sayfa Aralığı: 470 - 477 Metin Dili: İngilizce DOI: 10.5137/1019-5149.JTN.24336-18.0 İndeks Tarihi: 08-09-2020
Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?
Öz: AIM: To investigate the effect of dabigatran, a new oral anticoagulant, on human primary cell cultures isolated from intactintervertebral disc tissue.MATERIAL and METHODS: Cell cultures were prepared from tissues obtained from six cases who had undergone surgery dueto spinal trauma. Dabigatran, an active pharmacological agent, was applied to intact annulus fibrosus (AF)/nucleus pulposus (NP)primary cell cultures from the study group. After performing cell viability, toxicity, and proliferation tests on all cultures in the controland study groups, the surface morphologies of the samples were evaluated. Subsequently, chondroadherin (CHAD), cartilageoligomeric matrix protein (COMP), and matrix metalloproteinase (MMP)-13 and -19 expressions were measured via a real-timepolymerase chain reaction (RT-PCR). Data were analyzed statistically.RESULTS: In the proliferation assays performed on the 20th day of the study, cells in the dabigatran-supplemented group werereported to have lost 46.37% more viability than those in the control group. Expressions of all genes examined except MMP-13 wereevaluated in the control group by time, but in contrast to the control group results, COMP and MMP-19 gene expressions decreasedin the dabigatran-treated group. No CHAD or MMP-13 expression was noted in these cultures.CONCLUSION: The potential for a systemically applied drug to accumulate in tissue and negatively affect surrounding tissues andmicrostructures must be emphasized.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Akyuva Y, Kaplan N, Yilmaz I, Ozbek H, Sirin DY, Karaarslan N, Guler O, Ates O: Delivering growth factors through a polymeric scaffold to cell cultures containing both nucleus pulposus and annulus fibrosus. Turk Neurosurg 29(2):180-193, 2019
- 2. Akyuva Y, Karaarslan N, Yilmaz I, Ozbek H, Sirin DY, Gurbuz MS, Kaya YE, Kaplan N, Ates O: How scaffolds, which are polymeric drug delivery systems allowing controlled release, can be tested in human primary nucleus pulposus and annulus fibrosus cell cultures? Merit Res J Med Med Sci 5:477-487, 2017
- 3. Bastiaans J, Mulder VC, van Meurs JC, Smits-TeNijenhuis M, van Holten-Neelen C, van Hagen PM, Dik WA: Dabigatran inhibits intravitreal thrombin activity. Acta Ophthalmol 96(5):452-458, 2018
- 4. Beyer-Westendorf J: What have we learned from real-world NOAC studies in venous thromboembolism treatment? Thromb Res 163:83-91, 2018
- 5. Briggs MD, Rasmussen IM, Weber JL, Yuen J, Reinker K, Garber AP, Rimoin DL, Cohn DH: Genetic linkage of mild pseudoachondroplasia (PSACH) to markers in the pericentromeric region of chromosome 19. Genomics 18:656- 660, 1993
- 6. Castle J, Farnie G, Kirwan CC: PO-11 - Thrombin and cancer stem-like cells: In vitro support for breast cancer anticoagulation. Thromb Res 140:80, 2016
- 7. Chen FH, Herndon ME, Patel N, Hecht JT, Tuan RS, Lawler J: Interaction of cartilage oligomeric matrix protein/ thrombospondin 5 with aggrecan. J Biol Chem 282:24591- 24598, 2007
- 8. Chu DK, Hillis CM, Leong DP, Anand SS, Siegal DM: Benefits and risks of antithrombotic therapy in essential thrombocythemia: A systematic review. Ann Intern Med 167:170-180, 2017
- 9. Connolly SJ, Ezekowitz MD, Yusuf S, Eikelboom J, Oldgren J, Parekh A, Pogue J, Reilly PA, et al: Dabigatran versus warfarin in patients with atrial fibrillation. N Engl J Med 361:1139- 11351, 2009
- 10. Dhar A, Sadiq F, Anstee QM, Levene AP, Goldin RD, Thursz MR: Thrombin and factor Xa link the coagulation system with liver fibrosis. BMC Gastroenterol 18:60, 2018
- 11. Franchini M, Mannucci PM: Direct oral anticoagulants and venous thromboembolism. Eur Respir Rev 25: 295-302, 2016
- 12. Goode AP, Marshall SW, Kraus VB, Renner JB, Stürmer T, Carey TS, Irwin DE, Jordan JM: Association between serum and urine biomarkers and lumbar spine individual radiographic features: The Johnston County Osteoarthritis Project. Osteoarthritis Cartilage 20:1286-1293, 2012
- 13. Gumustas SA, Yilmaz I, Isyar M, Sirin DY, Batmaz AG, Ugras AA, Oznam K, Ciftci Z, Mahirogullari M: Assessing the negative impact of phenyl alkanoic acid derivative, a frequently prescribed drug for the suppression of pain and inflammation, on the differentiation and proliferation of chondrocytes. J Orthop Surg Res 11:70, 2016
- 14. Guzelant AY, Isyar M, Yilmaz I, Sirin DY, Cakmak S, Mahirogullari M: Are chondrocytes damaged when rheumatologic inflammation is suppressed? Drug Chem Toxicol 40:13-23, 2017
- 15. Haleem-Smith H, Calderon R, Song Y, Tuan RS, Chen FH: Cartilage oligomeric matrix protein enhances matrix assembly during chondrogenesis of human mesenchymal stem cells. J Cell Biochem 113:1245-1252, 2012
- 16. Heidbuchel H, Verhamme P, Alings M, Antz M, Hacke W, Oldgren J, Sinnaeve P, Camm AJ, Kirchhof P: European Heart Rhythm Association pratical guide on the use of new oral anticoagulants in patients with non-valvular atrial fibrillation. Europace 15:625-651, 2013
- 17. Karaarslan N, Batmaz AG, Yilmaz I, Ozbek H, Caliskan T, Sirin DY, Kaplan N, Oznam K, Ates O: Do aryl acetic acid derivative analgesic and anti-inflammatory drugs affect proliferation and differentiation in primary cell cultures formed from human cartilage tissue? Exp Ther Med 16(3):1647-1654, 2018
- 18. Karaarslan N, Yilmaz I, Akgun FS, Caliskan T, Simsek AT, Kaplan N, Kaya YE, Sirin DY, Ozbek H, Ates O: Which pharmacological agents may be used to establish healthy, proliferating human primer nucleus pulposus / annulus fibrosus cell cultures? Systematic evaluation of our experience in the light of literature. Merit Res J Med Med Sci 6:100-110, 2018
- 19. Karaarslan N, Yilmaz I, Ozbek H, Sirin Yasar D, Kaplan N, Akyuva Y, Gonultas A, Ates O: Are specific gene expressions of extracellular matrix and nucleus pulposus affected by primary cell cultures prepared from intact or degenerative intervertebral disc tissues? Turk Neurosurg 29(1):43-52, 2019
- 20. Karaarslan N, Yilmaz I, Sirin DY, Ozbek H, Kaplan N, Kaya YE, Akyuva Y, Gurbuz MS, Oznam K, Ates O: Does pregabalin used in the treatment of neuropathic pain damage intervertebral disc tissue? Exp Ther Med 16:1259-1265, 2018
- 21. Kennedy B, Gargoum FS, Kennedy L, Khan F, Curran DR, O’Connor TM: Emerging anticoagulants. Curr Med Chem 19: 3388-3416, 2012
- 22. Kwon WK, Moon HJ, Kwon TH, Park YK, Kim JH: The role of hypoxia in angiogenesis and extracellular matrix regulation of intervertebral disc cells during inflammatory reactions. Neurosurgery 81:867-875, 2017
- 23. Lau WCY, Li X, Wong ICK, Man KKC, Lip GYH, Leung WK, Siu CW, Chan EW: Bleeding-related hospital admissions and 30- day read missions in patients with non-valvularatrial fibrillation treated with dabigatran versus warfarin. J Thromb Haemost 15:1923-1933, 2017
- 24. Mao SH, Qian BP, Shi B, Zhu ZZ, Qiu Y: Quantitative evaluation of the relationship between COMP promoter methylation and the susceptibility and curve progression of adolescent idiopathic scoliosis. Eur Spine J 27: 272-277, 2018
- 25. Sirin DY, Kaplan N, Yilmaz I, Karaarslan N, Ozbek H, Akyuva Y, Kaya YE, Oznam K, Akkaya N, Guler O, Akkaya S, Mahirogullari M: The association between different molecular weights of hyaluronic acid and CHAD, HIF-1α, COL2A1 expression in chondrocyte cultures. Exp Ther Med 15:4205-4212,2018
- 26. Sirin DY, Karaarslan N: Evaluation of the effects of pregabalin on chondrocyte proliferation and CHAD, HIF-1α, and COL2A1 gene expression. Arch Med Sci 14(6):1340–1347, 2018
- 27. Trivedi A, Zhang H, Ekeledo A, Lee S, Werb Z, Plant GW, Noble-Haeusslein LJ: Deficiency in matrix metalloproteinase-2 results in long-term vascular instability and regression in the injured mouse spinal cord. Exp Neurol 284:50-62, 2016
- 28. Vadivelu S, Stewart TJ, Qu Y, Horn K, Liu S, Li Q, Silver J, McDonald JW: NG2+ progenitors derived from embryonic stem cells penetrate glial scar and promote axonal outgrowth into white matter after spinal cord injury. Stem Cells Transl Med 4: 401-411, 2015
- 29. Vianello F, Sambado L, Goss A, Fabris F, Prandoni P: Dabigatran antagonizes growth, cell-cycle progression, migration, and endothelial tube formation induced by thrombin in breast and glioblastoma cell lines. Cancer Med 5:2886-2898, 2016
| APA | KAPLAN N, KARAARSLAN N, Yılmaz İ, SIRIN D, AKGUN F, CALISKAN T, SIMSEK A, özbek h (2019). Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?. , 470 - 477. 10.5137/1019-5149.JTN.24336-18.0 |
| Chicago | KAPLAN Necati,KARAARSLAN Numan,Yılmaz İbrahim,SIRIN Duygu YASAR,AKGUN FERIDE SINEM,CALISKAN Tezcan,SIMSEK Abdullah Talha,özbek hanefi Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?. (2019): 470 - 477. 10.5137/1019-5149.JTN.24336-18.0 |
| MLA | KAPLAN Necati,KARAARSLAN Numan,Yılmaz İbrahim,SIRIN Duygu YASAR,AKGUN FERIDE SINEM,CALISKAN Tezcan,SIMSEK Abdullah Talha,özbek hanefi Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?. , 2019, ss.470 - 477. 10.5137/1019-5149.JTN.24336-18.0 |
| AMA | KAPLAN N,KARAARSLAN N,Yılmaz İ,SIRIN D,AKGUN F,CALISKAN T,SIMSEK A,özbek h Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?. . 2019; 470 - 477. 10.5137/1019-5149.JTN.24336-18.0 |
| Vancouver | KAPLAN N,KARAARSLAN N,Yılmaz İ,SIRIN D,AKGUN F,CALISKAN T,SIMSEK A,özbek h Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?. . 2019; 470 - 477. 10.5137/1019-5149.JTN.24336-18.0 |
| IEEE | KAPLAN N,KARAARSLAN N,Yılmaz İ,SIRIN D,AKGUN F,CALISKAN T,SIMSEK A,özbek h "Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?." , ss.470 - 477, 2019. 10.5137/1019-5149.JTN.24336-18.0 |
| ISNAD | KAPLAN, Necati vd. "Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?". (2019), 470-477. https://doi.org/10.5137/1019-5149.JTN.24336-18.0 |
| APA | KAPLAN N, KARAARSLAN N, Yılmaz İ, SIRIN D, AKGUN F, CALISKAN T, SIMSEK A, özbek h (2019). Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?. Turkish Neurosurgery, 29(4), 470 - 477. 10.5137/1019-5149.JTN.24336-18.0 |
| Chicago | KAPLAN Necati,KARAARSLAN Numan,Yılmaz İbrahim,SIRIN Duygu YASAR,AKGUN FERIDE SINEM,CALISKAN Tezcan,SIMSEK Abdullah Talha,özbek hanefi Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?. Turkish Neurosurgery 29, no.4 (2019): 470 - 477. 10.5137/1019-5149.JTN.24336-18.0 |
| MLA | KAPLAN Necati,KARAARSLAN Numan,Yılmaz İbrahim,SIRIN Duygu YASAR,AKGUN FERIDE SINEM,CALISKAN Tezcan,SIMSEK Abdullah Talha,özbek hanefi Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?. Turkish Neurosurgery, vol.29, no.4, 2019, ss.470 - 477. 10.5137/1019-5149.JTN.24336-18.0 |
| AMA | KAPLAN N,KARAARSLAN N,Yılmaz İ,SIRIN D,AKGUN F,CALISKAN T,SIMSEK A,özbek h Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?. Turkish Neurosurgery. 2019; 29(4): 470 - 477. 10.5137/1019-5149.JTN.24336-18.0 |
| Vancouver | KAPLAN N,KARAARSLAN N,Yılmaz İ,SIRIN D,AKGUN F,CALISKAN T,SIMSEK A,özbek h Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?. Turkish Neurosurgery. 2019; 29(4): 470 - 477. 10.5137/1019-5149.JTN.24336-18.0 |
| IEEE | KAPLAN N,KARAARSLAN N,Yılmaz İ,SIRIN D,AKGUN F,CALISKAN T,SIMSEK A,özbek h "Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?." Turkish Neurosurgery, 29, ss.470 - 477, 2019. 10.5137/1019-5149.JTN.24336-18.0 |
| ISNAD | KAPLAN, Necati vd. "Are Intervertebral Disc Tissue Cells Damaged When Attempting to Prevent Thrombus Formation Using Dabigatran, A New Oral Anticoagulant?". Turkish Neurosurgery 29/4 (2019), 470-477. https://doi.org/10.5137/1019-5149.JTN.24336-18.0 |
