Wheat seed gall nematode [Anguina tritici Thorne, 1949 (Nematoda: Anguinidae)] is one of the plant-parasitic nematodes (PPNs) causing economic yield losses in wheat-growing areas. A. tritici is a seed born nematode and can survive in stored seed for many years under dry conditions. Some plants and botanical products were proved to be effective to PPNs control in terms of alternative nematode management tactics. In this study, silver nanoparticles prepared using Lantana camara L. (Lamiales: Verbenaceae) aqueous extract (AgNPs) as an eco-friendly control of nematodes were evaluated against A. tritici in in vitro conditions (Petri dishes experiment). Silver nanoparticles using L. camara aqueous extract were used in 3 different concentrations [42 ppm (0.25 mM), 84 ppm (0.5 mM) and 168 ppm (1 mM)] were used. The experiments were conducted as randomized block parcel design with four replicates and four characters [42 ppm, 84 ppm, 168 ppm applications, and positive control C (+)] in Atatürk Central Horticultural Research Institute (Yalova, Turkey). A. tritici individuals were transferred into sterilized Petri dishes in four replicates and kept at 28±2 °C. A. tritici individuals were considered dead if there were no motion when touched with a fine needle. All the concentrations showed different of antinematodal activity. Silver nanoparticles at concentrations of 168 ppm (1 mM) prepared using L. camara aqueous extract caused 97% A. tritici mortality in 72 hours.