Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats

UR, Koray; BENGU, Aydin Sukru; GERGIN, Ozlem Oz; DEMİRÖZ, SERDAR; Erdem, Sevki; ULUCAN, Aykut; UR, Feyza DEMIRALIN

doi:10.5137/1019-5149.JTN.25891-19.2

Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats

Serdar DEMİRÖZ, (Bingöl Devlet Hastanesi, Ortopedi ve Travmatoloji Kliniği, Bingöl, Türkiye)

Koray UR, (Bingol Devlet Hastanesi, Nöroşirurji Kliniği, Bingöl, Türkiye)

Aykut ULUCAN, (Bingol Üniversitesi Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Anabilim Dalı, Patoloji Laboratuvar Programı, Bingol, Türkiye)

Aydin Sukru BENGU, (Bingol Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Anabilim Dalı, Tıbbi Laboratuvar Programı, Bingol, Türkiye)

Feyza DEMIRALIN UR, (Bingol Devlet Hastanesi, Nöroloji Kliniği, Bingöl, Türkiye)

Ozlem Oz GERGIN, (Kayseri Eğitim ve Araştırma Hastanesi, Anesteziyoloji ve Reanimasyon Kliniği, Kayseri, Türkiye)

Şevki ERDEM (Medicana Kadıköy Hastanesi, Ortopedi ve Travmatoloji Kliniği, İstanbul, Türkiye)

Turkish Neurosurgery

5 0

Yıl: 2019 Cilt: 29 Sayı: 5 Sayfa Aralığı: 718 - 723 Metin Dili: İngilizce DOI: 10.5137/1019-5149.JTN.25891-19.2 İndeks Tarihi: 10-09-2020

Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats

Öz:

AIM: To evaluate the effects of lacosamide on traumatic spinal cord injury (SCI) in rats.MATERIAL and METHODS: A total of 28 male Wistar albino rats, each weighing 300–350 g, were included. They were randomlyassigned to four groups. In Group 1, only a laminectomy was performed; in Group 2, SCI was performed after laminectomy; inGroup 3, SCI was performed after laminectomy followed by lacosamide administration, and in Group 4, SCI was performed afterlaminectomy followed by physiological saline administration. After 48 hours, all animals were sacrificed, blood samples were drawn,and their spinal cords were removed. The serum levels of catalase, glutathione peroxidase (GPx), superoxide dismutase (SOD) andmalondialdehyde (MDA) were measured, and the spinal cord specimens were examined for neuronal degeneration (PND).RESULTS: The MDA level was the lowest and the antioxidant enzyme levels were the highest in Group 3. There were statisticallysignificant differences between Group 3 and the others in their PND score, serum MDA, SOD, GPX and catalase levels (p<0.05).CONCLUSION: Lacosamide has a neuroprotective effect in SCI in rats that is related to its ability to decrease the production ofreactive oxygen species by increasing antioxidant enzyme expression, inhibit lipid peroxidation and attenuate glial cell activation.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Ahn JY, Yan BC, Park JH, Ahn JH, Lee DH, Kim IH, Cho JH, Chen BH, Lee JC, Cho YS, Shin MC, Cho JH, Hong S, Won MH, Kim SK: Novel antiepileptic drug lacosamide exerts neuroprotective effects by decreasing glial activation in the hippocampus of a gerbil model of ischemic stroke. ExpTher Med 10:2007–2014, 2015
2. Bell MT, Puskas F, Agoston VA, Cleveland JC Jr, Freeman KA, Gamboni F, Herson PS, Meng X, Smith PD, Weyant MJ, Fullerton DA, Reece TB: Toll-like receptor 4 dependent microglial activation mediates spinal cord ischemiare perfusion injury. Circulation 128 Suppl 1:152-156, 2013
3. Celik H, Karatay M, Erdem Y, Yildirim AE, Sertbas I, Karatay E, Kul H, Guvenc Y, Koksal I, Menekse G, Alagoz F, Kertmen HH, Caydere M:. The biochemical, histopathological and clinical comparison of the neuroprotective effects of subcutaneous adalimumab and intravenous methylprednisolone in an experimental compressive spinal cord trauma model. Turk Neurosurg 26:622-631, 2016
4. Choi HY, Park JH, Chen BH, Shin BN, Lee YL, Kim IH, Cho JH, Lee TK, Lee JC, Won MH, Ahn JH, Tae HJ, Yan BC, Hwang IK, Cho JH, Kim YM, Kim SK:Increases of catalase and glutathione peroxidase expressions by lacosamide pretreatment contributes to neuroprotection against experimentally induced transient cerebral ischemia. Neurochem Res 41:2380-2390, 2016
5. De Girolami U, Frosch MP, Tator CH: Regional neuropathology diseases of the spinal cord and vertebral column. In: Graham DI, Lantos PL, (eds). Greenfield’s Neuropathology, 7th ed. London: Arnold, 2002:1063–1101
6. Erol FS, Kaplan M, Tiftikci M, Yakar H, Ozercan I, Ilhan N, Topsakal C: Comparison of the effects of octreotide and melatonin in preventing nerve injury in rats with experimental spinal cord injury. J Clin Neurosci 15:784-790, 2008
7. Faden AI: Therapeutic approaches to spinal cord injury. Adv Neurol 72: 377-386, 1997
8. Faden AI, Salzman S: Pharmacological strategies in CNS trauma. Trends Pharmacol Sci 13:2935, 1992
9. Fedchenko N, Reifenrath J: Different approaches for interpretation and reporting of immunohistochemistry analysis results in the bone tissue – a review. Diagn Pathol 9:221, 2014
10. Gal P, Kravcukova P, Mokry M, Kluchova D: Chemokines as possible targets in modulation of the secondary damage after acute spinal cord injury: A review. Cell Mol Neurobiol 29:1025- 1035, 2009
11. Hall ED, Braughler JM: Central nervous system trauma and stroke. II. Physiological and pharmacological evidence for involvement of oxygen radicals and lipid peroxidation. Free Radic Biol Med 6: 303-313, 1989
12. Hao JX, Stohr T, Selve N, Wiesenfeld-Hallin Z, Xu XJ: Lacosamide, a new antiepileptic, alleviates neuropathic painlike behaviors in rat models of spinal cord or trigeminal nerve injury. Eur J Pharmacol 553:135–140, 2006
13. Ildan F, Polat S, Oner A, Isbir T, Göcer AI, Tap O, Kaya M, Karadayi A: Effects of naloxone on sodiumand potassiumactivated and magnesium-dependent adenosine-5’- triphosphatase activity and lipid peroxidation and early ultrastructural findings after experimental spinal cord injury. Neurosurgery 36:797-805, 1995
14. Kaptanoglu E, Solaroglu I, Okutan O, Surucu HS, Akbiyik F, Beskonakli E: Erythropoietin exerts neuroprotection after acute spinal cord injury in rats: Effect on lipid peroxidation and early ultrastructural findings. Neurosurg Rev 27:113-120, 2004
15. Kaptanoglu E, Tuncel M, Palaoglu S, Konan A, Demirpence E, Kilinc K: Comparison of the effects of melatonin and methylprednisolone in experimental spinal cord injury. J Neurosurg 93 Suppl 1:77-84, 2000
16. Koc RK, Akdemir H, Karakücük EI, Oktem IS, Menkü A: Effect of methylprednisolone, tirilazadmesylate and vitamin E on lipid peroxidation after experimental spinal cord injury. Spinal Cord 37:29-32, 1999
17. Kurtoglu T, Basoglu H, Ozkisacik EA, Cetin NK, Tataroglu C, Yenisey C, Discigil B: Effects of cilostazol on oxidative stress, systemic cytokine release, and spinal cord injury in a rat model of transient aortic occlusion. Ann Vasc Surg 28:479-488, 2014
18. Marques SA, Garcez VF, Del Bel EA, Martinez AM: A simple, inexpensive and easily reproducible model of spinal cord injury in mice: Morphological and functional assessment. Journal of Neuroscience Methods 177:183–193, 2009
19. Menekse G, Daglioglu E, Nacar OA, Polat E, Ozdol C, Dalgic A, Take G, Okten AI, Belen AD: The neuroprotective effects of rituximab in rat spinal cord injury model: An immunohistochemical study. Turk Neurosurg 23:783-790, 2013
20. Oxidative stress. In: Halliwell B, Gutteridge JM (eds). Free Radicals in Biology and Medicine. 3rd ed. New York: Oxford University Press, 1999:246-350
21. Pirouzmand F: Epidemiological trends of spine and spinal cord injuries in the largest Canadian adult trauma center from 1986 to 2006. J Neurosurg Spine 12:131–140, 2010
22. Pitkanen A, Immonen R, Ndode-Ekane X, Gröhn O, Stöhr T, Nissinen J: Effect of lacosamide on structural damage and functional recovery after traumatic brain injury in rats. Epilepsy Res 108:653–665, 2014
23. Rowland JW, Hawryluk GW, Kwon B, Fehlings MG: Current status of acute spinal cord injury pathophysiology and emerging therapies: Promise on the horizon. Neurosurg Focus 25:E2, 2008
24. Roy Choudhury G, Ryou MG, Poteet E, Wen Y, He R, Sun F, Yuan F, Jin K, Yang SH: Involvement of p38 MAPK in reactive astrogliosis induced by ischemic stroke. Brain Res 1551: 45- 58, 2014
25. Sayer FT, Kronvall E, Nilsson OG: Methylprednisolone treatment in acute spinal cord injury: The myth challenged through a structured analysis of published literature. Spine J 6:335–343, 2006
26. Sengul G, Coban MK, Cakir M, Coskun S, Aksoy H, Hacimuftuoglu A, Saruhan F, Calik M: Neuroprotective effect of acute interferon-beta 1B treatment after spinal cord injury. Turk Neurosurg 23:45-49, 2013
27. Solmaz V, Aksoy D, Yılmaz M, Eser E, Erbas O: Demonstration of ameliorative effect of lacosamide: In a rat model of sepsis induced criticalillness polyneuropathy. Neurol Res 37:797- 802, 2015
28. Tator CH, Fehlings MG: Review of the secondary injury theory of acute spinal cord trauma with emphasis on vascular mechanisms. J Neurosurg 75:15-26, 1991
29. Toklu HZ, Hakan T, Celik H, Biber N, Erzik C, Ogunc AV, Akakin D, Cikler E, Cetinel S, Ersahin M, Sener G: Neuroprotective effects of alpha-lipoic acid in experimental spinal cord injury in rats. J Spinal Cord Med 33:401-409, 2010
30. Tufan K, Oztanir N, Ofluoglu E, Ozogul C, Uzum N, Dursun A, Pasaoglu H, Pasaoglu A: Ultrastructure protection and attenuation of lipid peroxidation after blockade of presynaptic release of glutamate by lamotrigine in experimental spinal cord injury. Neurosurg Focus 25:E6, 2008
31. Usul H, Arslan E, Cansever T, Cobanoglu U, Baykal S: Effects of clotrimazole on experimental spinal cord ischemia/ reperfusion injury in rats. Spine 33:2863-2867, 2008
32. Yang YH, Wang Z, Zheng J, Wang R: Protective effects of gallic acid against spinal cord injury-induced oxidative stress. Mol Med Rep 12:3017-3024, 2015
33. Young W: Medical treatments of acute spinal cord injury. J Neurol Neurosurg Psychiatry 55:635-639,1992

APA	DEMİRÖZ S, UR K, ULUCAN A, BENGU A, UR F, GERGIN O, Erdem S (2019). Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats. , 718 - 723. 10.5137/1019-5149.JTN.25891-19.2
Chicago	DEMİRÖZ SERDAR,UR Koray,ULUCAN Aykut,BENGU Aydin Sukru,UR Feyza DEMIRALIN,GERGIN Ozlem Oz,Erdem Sevki Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats. (2019): 718 - 723. 10.5137/1019-5149.JTN.25891-19.2
MLA	DEMİRÖZ SERDAR,UR Koray,ULUCAN Aykut,BENGU Aydin Sukru,UR Feyza DEMIRALIN,GERGIN Ozlem Oz,Erdem Sevki Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats. , 2019, ss.718 - 723. 10.5137/1019-5149.JTN.25891-19.2
AMA	DEMİRÖZ S,UR K,ULUCAN A,BENGU A,UR F,GERGIN O,Erdem S Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats. . 2019; 718 - 723. 10.5137/1019-5149.JTN.25891-19.2
Vancouver	DEMİRÖZ S,UR K,ULUCAN A,BENGU A,UR F,GERGIN O,Erdem S Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats. . 2019; 718 - 723. 10.5137/1019-5149.JTN.25891-19.2
IEEE	DEMİRÖZ S,UR K,ULUCAN A,BENGU A,UR F,GERGIN O,Erdem S "Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats." , ss.718 - 723, 2019. 10.5137/1019-5149.JTN.25891-19.2
ISNAD	DEMİRÖZ, SERDAR vd. "Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats". (2019), 718-723. https://doi.org/10.5137/1019-5149.JTN.25891-19.2

APA	DEMİRÖZ S, UR K, ULUCAN A, BENGU A, UR F, GERGIN O, Erdem S (2019). Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats. Turkish Neurosurgery, 29(5), 718 - 723. 10.5137/1019-5149.JTN.25891-19.2
Chicago	DEMİRÖZ SERDAR,UR Koray,ULUCAN Aykut,BENGU Aydin Sukru,UR Feyza DEMIRALIN,GERGIN Ozlem Oz,Erdem Sevki Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats. Turkish Neurosurgery 29, no.5 (2019): 718 - 723. 10.5137/1019-5149.JTN.25891-19.2
MLA	DEMİRÖZ SERDAR,UR Koray,ULUCAN Aykut,BENGU Aydin Sukru,UR Feyza DEMIRALIN,GERGIN Ozlem Oz,Erdem Sevki Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats. Turkish Neurosurgery, vol.29, no.5, 2019, ss.718 - 723. 10.5137/1019-5149.JTN.25891-19.2
AMA	DEMİRÖZ S,UR K,ULUCAN A,BENGU A,UR F,GERGIN O,Erdem S Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats. Turkish Neurosurgery. 2019; 29(5): 718 - 723. 10.5137/1019-5149.JTN.25891-19.2
Vancouver	DEMİRÖZ S,UR K,ULUCAN A,BENGU A,UR F,GERGIN O,Erdem S Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats. Turkish Neurosurgery. 2019; 29(5): 718 - 723. 10.5137/1019-5149.JTN.25891-19.2
IEEE	DEMİRÖZ S,UR K,ULUCAN A,BENGU A,UR F,GERGIN O,Erdem S "Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats." Turkish Neurosurgery, 29, ss.718 - 723, 2019. 10.5137/1019-5149.JTN.25891-19.2
ISNAD	DEMİRÖZ, SERDAR vd. "Neuroprotective Effects of Lacosamide in Experimental Traumatic Spinal Cord Injury in Rats". Turkish Neurosurgery 29/5 (2019), 718-723. https://doi.org/10.5137/1019-5149.JTN.25891-19.2