Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures

Bilgin, Mehmet Dincer; Carhan, Ahmet; KOZACI, LEYLA DIDEM; Kafienah, Wael; ÇALIŞKAN, Şerife Gökçe

Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures

Leyla KOZACI, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Ankara, Türkiye)

Mehmet Dinçer BİLGİN, (Adnan Menderes Üniversitesi, Tıp Fakültesi, Biyofizik Anabilim Dalı, Aydın, Türkiye)

Ahmet ÇARHAN, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Ankara, Türkiye)

Şerife ÇALIŞKAN, (Adnan Menderes Üniversitesi, Fen Edebiyat Fakültesi, Fizik Bölümü, Aydın, Türkiye)

Wael KAFIENAH (University of Bristol, School of Cellular and Molecular Medicine, Bristol, UK)

Cyprus Journal of Medical Sciences

3 1

Yıl: 2019 Cilt: 4 Sayı: 3 Sayfa Aralığı: 201 - 207 Metin Dili: İngilizce İndeks Tarihi: 09-09-2020

Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures

Öz:

BACKGROUND/AIMSTissue engineering approaches have provided treatment options for patients with limited cartilage repair capacity. Most of these approaches rely on isolating and expanding chondrocytes in vitro. Mechanical stress, ultrasound, and electric and electromagnetic fields (EMFs) can be used to stimulate cartilage repair. EMF has been used in the management of conditions such as arthritis and fractures. Most of the previous studies have focused on low-frequency pulsed EMF (PEMF). The aim of the present study was to investigate the effects of low-frequency continuous (sinusoidal) EMF (CEMF) versus PEMF on chondrocytes.MATERIAL and METHODSChondrocytes from bovine nasal cartilage were exposed to low-frequency CEMF versus PEMF, and the proliferation and differentiation capacities of these chondrocytes were determined. The effects of EMFs on retinoic acid receptor beta and transforming growth factor beta (TGF-β) expressions were investigated using quantitative reverse transcription polymerase chain reaction.RESULTSOur observations suggested that there was no difference between the effects of PEMF and CEMF exposure on the proliferation and differentiation capacities of chondrocytes.CONCLUSIONEMF-mediated proliferation of chondrocytes requires the presence of growth factors, especially insulin-like growth factor, in the environment to maintain the chondrogenic phenotype; furthermore, the EMF effect on chondrocytes is independent of TGF-β.

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APA	KOZACI L, Bilgin M, Carhan A, ÇALIŞKAN Ş, Kafienah W (2019). Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures. , 201 - 207.
Chicago	KOZACI LEYLA DIDEM,Bilgin Mehmet Dincer,Carhan Ahmet,ÇALIŞKAN Şerife Gökçe,Kafienah Wael Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures. (2019): 201 - 207.
MLA	KOZACI LEYLA DIDEM,Bilgin Mehmet Dincer,Carhan Ahmet,ÇALIŞKAN Şerife Gökçe,Kafienah Wael Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures. , 2019, ss.201 - 207.
AMA	KOZACI L,Bilgin M,Carhan A,ÇALIŞKAN Ş,Kafienah W Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures. . 2019; 201 - 207.
Vancouver	KOZACI L,Bilgin M,Carhan A,ÇALIŞKAN Ş,Kafienah W Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures. . 2019; 201 - 207.
IEEE	KOZACI L,Bilgin M,Carhan A,ÇALIŞKAN Ş,Kafienah W "Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures." , ss.201 - 207, 2019.
ISNAD	KOZACI, LEYLA DIDEM vd. "Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures". (2019), 201-207.

APA	KOZACI L, Bilgin M, Carhan A, ÇALIŞKAN Ş, Kafienah W (2019). Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures. Cyprus Journal of Medical Sciences, 4(3), 201 - 207.
Chicago	KOZACI LEYLA DIDEM,Bilgin Mehmet Dincer,Carhan Ahmet,ÇALIŞKAN Şerife Gökçe,Kafienah Wael Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures. Cyprus Journal of Medical Sciences 4, no.3 (2019): 201 - 207.
MLA	KOZACI LEYLA DIDEM,Bilgin Mehmet Dincer,Carhan Ahmet,ÇALIŞKAN Şerife Gökçe,Kafienah Wael Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures. Cyprus Journal of Medical Sciences, vol.4, no.3, 2019, ss.201 - 207.
AMA	KOZACI L,Bilgin M,Carhan A,ÇALIŞKAN Ş,Kafienah W Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures. Cyprus Journal of Medical Sciences. 2019; 4(3): 201 - 207.
Vancouver	KOZACI L,Bilgin M,Carhan A,ÇALIŞKAN Ş,Kafienah W Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures. Cyprus Journal of Medical Sciences. 2019; 4(3): 201 - 207.
IEEE	KOZACI L,Bilgin M,Carhan A,ÇALIŞKAN Ş,Kafienah W "Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures." Cyprus Journal of Medical Sciences, 4, ss.201 - 207, 2019.
ISNAD	KOZACI, LEYLA DIDEM vd. "Effects of Low-Frequency Electromagnetic Fields on Chondrocytes in Short-Term Cultures". Cyprus Journal of Medical Sciences 4/3 (2019), 201-207.