Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department

Ozhasenekler, Ayhan; Tanrıverdi, Fatih; Kurtoglu Celık, Gulhan; Pamukçu Günaydın, Gül; Kahraman, Fatih Ahmet; Yıldırım, Çağdaş; Gökhan, Servan; EREL, Ozcan

Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department

Şervan GÖKHAN, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Acil Tıp Anabilim Dalı, Ankara, Türkiye)

Fatih TANRIVERDİ, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Acil Tıp Anabilim Dalı, Ankara, Türkiye)

Gül GÜNAYDIN, (Ankara Atatürk Eğitim ve Araştırma Hastanesi, Acil Tıp Kliniği, Ankara, Türkiye)

Çağdaş YILDIRIM, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Acil Tıp Kliniği, Ankara, Türkiye)

Fatih KAHRAMAN, (Ankara Atatürk Eğitim ve Araştırma Hastanesi, Acil Tıp Kliniği, Ankara, Türkiye)

Ayhan ÖZHASENEKLER, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Acil Tıp Kliniği, Ankara, Türkiye)

Gülhan ÇELİK, (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Acil Tıp Kliniği, Ankara, Türkiye)

Özcan EREL (Ankara Yıldırım Beyazıt Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye)

Cyprus Journal of Medical Sciences

1 0

Yıl: 2019 Cilt: 4 Sayı: 1 Sayfa Aralığı: 19 - 23 Metin Dili: İngilizce İndeks Tarihi: 10-09-2020

Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department

Öz:

BACKGROUND/AIMSThe aim of this study was to investigate the diagnostic utility of thiol/disulfide homeostasis parameters, which are oxidative stress markers, together with the neutrophil-to-lymphocyte ratio (NLR) in diagnosing of urinary tract infections (UTI) in the emergency department (ED).MATERIAL and METHODSThe study was conducted prospectively and included 63 patients who were admitted to the ED between May 15th, 2018, and October 1st, 2018, and 59 healthy subjects included as the control group. A new method developed by Erel and Neşelioğlu was used to measure the thiol/disulfide homeostasis parameters (thiol, disulfide, disulfide/native thiol, disulfide/total thiol, native thiol/total thiol), and the NLR was studied in the patient and control groups. In addition, the relationship between these parameters and the body temperature and urinary cultures in patients with UTI was investigated as well.RESULTSThe NLR, disulfide/native thiol, and disulfide/total thiol mean values were found to be significantly higher (p<0.001, 0.013 and 0.009, respectively) in patients with a UTI compared to the control group. Disulfide, disulfide/native thiol, and disulfide/total thiol mean values were found to be significantly higher (p<0.001, <0.001 and <0.001 respectively) in the group with a high body temperature compared to the group without high temperature. Native thiol and total thiol values were found to be significantly lower (p=0.048 and 0.020, respectively) in the group with a positive urine culture compared to the group with a negative urine culture.CONCLUSIONThiol/disulfide homeostasis parameters together with the NLR values are diagnostically useful in patients with UTI.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Stamm WE, Norrby SR. Urinary tract infections: disease panorama and challenges. J Infect Dis 2001; 183(suppl 1): 1-4. [CrossRef]
2. Sobel JD, Kaye D. Urinary tract infection. In: Mandell GL, Bennett JE, Dolin R(eds). Principles and Practice of Infectious Diseases. 4th ed. New York: Churchill Livingstone, 1995: 662-90
3. Gupta K, Stamm WE. Pathogenesis and managment of recurrent urinary tract infections in women. World J Urol 1999; 17: 415-20. [CrossRef]
4. Nielubowicz GR, Mobley HL. Host-pathogen interactions in urinary tract infection. Nat Rev Urol 2010; 7: 430-41. [CrossRef]
5. Öztürk U, İmamoğlu MA. Antibiotic Applications in Uncomplicated Urinary Tract Infections. Turk Urol Sem 2010; 1: 226-31. [CrossRef]
6. Rubin R, Shapiro ED, Andriole VT, Davis RJ, Stamm WE. General guidelines for the evaluation of new anti-infective drugs for treatment of urinary tract infection. Clin Infect Dis 1992; 15(suppl): 216-27. [CrossRef]
7. Stamm WE, Hooton TM. Managment of urinary tract infections in adults. N Engl J Med 1993; 329: 1328-34. [CrossRef]
8. Smith EA. Pyelonephritis, renal scarring and reflux nephropathy : a pediatric urologist’s perspective. Pediatr Radiol 2008; 38(suppl 1): S76-82. [CrossRef]
9. Grover S, Srivastava A, Lee R, Tewari AK, Te AE. Role of inflammation in bladder function and interstitial cystitis. Ther Adv Urol 2011; 3: 19-33. [CrossRef]
10. Shihamura T. Mechanisms of renal tissue destruction in an experimental acute pyelonephritis. Exp Mol Pathol 1981; 34: 34-42. [CrossRef]
11. Monga M, Roberts JA. The possible role of granulocyte elastase in renal damage from acute pyelonephritis. Pediatr Nephrol 1995; 9: 583-6. [CrossRef]
12. Tekin M, Konca C, Gulyuz A, Uckardes F, Turgut M. Is the mean platelet volume a predictive marker for the diagnosis of acute pyelonephritis in children? Clin Exp Nephrol 2015; 19: 688-93. [CrossRef]
13. Shaikh N, Borrell JL, Evron J, Leeflang MM. Procalcitonin, C-reactive protein and erythrocyte sedimantation rate fort he diagnosis of acute pyelonephritis in children. Cochrane Database Syst Rev 2015; 1: CD009185. [CrossRef]
14. Bhat T, Teli S, Rijal J, Bhat H, Raza M, Khoueiry G et al. Neutrophil to lymphocyte ratio and cardiovascular diseases:a review. Expert Rev Cardiovasc Ther 2013; 11: 55-9. [CrossRef]
15. Lowsby R, Gomes C, Jarman I, Lisboa P, Nee PA, Vardhan M et al. Neutrophil to lymphocyte count ratio as an early indicator of blood stream infection in the emergency department. Emerg Med J 2015; 32: 531-4. [CrossRef]
16. Bolat D, Topcu YF, Aydogdu O, Minareci S, Dincel C. Neutrophil to lymphocyte ratio as a predictor of early penile prosthesis implant infection. Int Urol Nephrol 2017; 49: 947-53. [CrossRef]
17. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014; 47: 326-32. [CrossRef]
18. Ellman G, Lysko H. A precise method for the determination of whole blood and plasma sulfhydryl groups. Anal Biochem 1979; 93: 98-102. [CrossRef]
19. Horton JW. Free radicals and lipid peroxidation mediated injury in burn trauma: The role of antioxidant therapy. Toxicology 2003; 189: 75-88. [CrossRef]
20. Biswas S, Chida AS, Rahman I. Redox modifications of proteinthiols:emerging roles in cell signaling. Biochem Pharmacol 2006; 71: 551-64. [CrossRef]
21. Kundi H, Erel Ö, Balun A, Çiçekçiog˘lu H, Cetin M, Kiziltunç E, et al. Association of thiol/disulfide ratio with syntax score in patients with NSTEMI. Scand Cardiovasc J 2015; 49: 95-100. [CrossRef]
22. Eren Y, Dirik E, Neselioglu S, Erel O. Oxidative stress and decreased thiol level in patients with migraine: cross-sectional study. Acta Neurol Belg 2015; 115: 643-9. [CrossRef]
23. Holub M, Beran O, Kasprikova N, Chalupa P. Neutrophil to lymphocyte count ratio as a biomarker of bacterial infections. Open Med 2012; 7: 258-61. [CrossRef]
24. Liu X, Shen Y, Wang H, Ge Q, Fei A, Pan S. Prognostic significance of neutrophil to lymphocyte ratio in patients with sepsis: a prospective observational study. Mediators Inflamm 2016; 2016: 8191254. [CrossRef]
25. Kara SS, Erel O, Demirdağ TB, Cura Yayla BC, Gulhan B, Neselioglu S, et al. Alteration of thiol-disulphide homeostasis in acute tonsillopharyngitis. Redox Rep 2017; 22: 205-9. [CrossRef]
26. Kolgelier S, Ergin M, Demir LS, Inkaya AC, Aktug Demir N, Alisik M, et al. Impaired Thiol-Disulfide Balance in Acute Brucellosis. Jpn J Infect Dis 2017; 70: 258-62. [CrossRef]
27. Nam KW, Kim TJ, Lee SJ, Kwon HM, Lee YS, Ko SB, et al. High neutrophil to lymphocyte ratio predicts stroke-associated pneumonia. Stroke 2018; 49: 1886-92. [CrossRef]
28. Han SR, Lee IR, Park SJ, Kim JH, Shin JI. Usefulness of neutrophil lymphocyte ratio in young children with febrile urinary tract infection. Korean J Pediatr 2016; 59: 139-44. [CrossRef]
29. Lee JW, Park SJ, Park KB, Yoo GH, Kim SS, Lee SM. Prediction of renal cortical defect and scar using neutrophil to lymphocyte ratio in children with febril urinary tract infection. Nuklearmedizin 2017; 56: 109-14. [CrossRef]
30. Parlak ES, Alisik M, Hezer H, Karalezli A, Hasanoglu HC, Erel O. Evaluation of dynamic thiol/disulphide redox state in community-acquired pneumonia. Saudi Med J 2018; 39: 495-9. [CrossRef]
31. Kundi H, Gök M, Çetin M. Association of thiol disulfide homeostasis with slow coronary flow. Scand Cardiovasc J 2016; 50: 213-7. [CrossRef]
32. Wyllie DH, Bowler ICJW, Peto TEA. Relation between lymphopenia and bacteremia in UK adults with medical emergencies. J Clin Pathol 2004; 57: 950-5. [CrossRef]
33. de Jager CPC, van Wijk PTL, Mathoera RB, de Jongh-Leuvenink J, van der Poll T, Wever PC. Lymphocytopenia and neutrophil-lympho22cyte count ratio predict bacteremia better than conventional infection markers in an emergency care unit. Crit Care 2010; 14: R192. [CrossRef]
34. Sarier, M., Demir, M., Duman, I., Yuksel, Y., Demirbas, A. Evaluation of ureteral stent colonization in live-donor renal transplant recipients. Transplant Proc 2017; 49: 415-9. [CrossRef]
35. Owrangi B, Masters N, Kuballa A, O’Dea C, Vollmerhausen TL, Katouli M. Invasion and translocation of uropathogenic Escherichia Coli isolated from urosepsis and patients with community-acquired urinary tract infection. Eur J Clin Microbiol Infect Dis 2018; 37: 833-9. [CrossRef]
36. Tufvesson E, Markstad H,Bozovic G, Ekberg M, Bjermer L. Inflammation and chronic colonization of Haemophilus Influenza in sputum in COPD patients related to the degree of emphysema and bronchiectasis in high resolution computed tomography. Int J Chron Obstruct Pulmon Dis 2017; 12: 3211-9. [CrossRef]

APA	Gökhan S, Tanrıverdi F, Pamukçu Günaydın G, Yıldırım Ç, Kahraman F, Ozhasenekler A, Kurtoglu Celık G, EREL O (2019). Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department. , 19 - 23.
Chicago	Gökhan Servan,Tanrıverdi Fatih,Pamukçu Günaydın Gül,Yıldırım Çağdaş,Kahraman Fatih Ahmet,Ozhasenekler Ayhan,Kurtoglu Celık Gulhan,EREL Ozcan Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department. (2019): 19 - 23.
MLA	Gökhan Servan,Tanrıverdi Fatih,Pamukçu Günaydın Gül,Yıldırım Çağdaş,Kahraman Fatih Ahmet,Ozhasenekler Ayhan,Kurtoglu Celık Gulhan,EREL Ozcan Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department. , 2019, ss.19 - 23.
AMA	Gökhan S,Tanrıverdi F,Pamukçu Günaydın G,Yıldırım Ç,Kahraman F,Ozhasenekler A,Kurtoglu Celık G,EREL O Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department. . 2019; 19 - 23.
Vancouver	Gökhan S,Tanrıverdi F,Pamukçu Günaydın G,Yıldırım Ç,Kahraman F,Ozhasenekler A,Kurtoglu Celık G,EREL O Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department. . 2019; 19 - 23.
IEEE	Gökhan S,Tanrıverdi F,Pamukçu Günaydın G,Yıldırım Ç,Kahraman F,Ozhasenekler A,Kurtoglu Celık G,EREL O "Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department." , ss.19 - 23, 2019.
ISNAD	Gökhan, Servan vd. "Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department". (2019), 19-23.

APA	Gökhan S, Tanrıverdi F, Pamukçu Günaydın G, Yıldırım Ç, Kahraman F, Ozhasenekler A, Kurtoglu Celık G, EREL O (2019). Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department. Cyprus Journal of Medical Sciences, 4(1), 19 - 23.
Chicago	Gökhan Servan,Tanrıverdi Fatih,Pamukçu Günaydın Gül,Yıldırım Çağdaş,Kahraman Fatih Ahmet,Ozhasenekler Ayhan,Kurtoglu Celık Gulhan,EREL Ozcan Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department. Cyprus Journal of Medical Sciences 4, no.1 (2019): 19 - 23.
MLA	Gökhan Servan,Tanrıverdi Fatih,Pamukçu Günaydın Gül,Yıldırım Çağdaş,Kahraman Fatih Ahmet,Ozhasenekler Ayhan,Kurtoglu Celık Gulhan,EREL Ozcan Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department. Cyprus Journal of Medical Sciences, vol.4, no.1, 2019, ss.19 - 23.
AMA	Gökhan S,Tanrıverdi F,Pamukçu Günaydın G,Yıldırım Ç,Kahraman F,Ozhasenekler A,Kurtoglu Celık G,EREL O Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department. Cyprus Journal of Medical Sciences. 2019; 4(1): 19 - 23.
Vancouver	Gökhan S,Tanrıverdi F,Pamukçu Günaydın G,Yıldırım Ç,Kahraman F,Ozhasenekler A,Kurtoglu Celık G,EREL O Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department. Cyprus Journal of Medical Sciences. 2019; 4(1): 19 - 23.
IEEE	Gökhan S,Tanrıverdi F,Pamukçu Günaydın G,Yıldırım Ç,Kahraman F,Ozhasenekler A,Kurtoglu Celık G,EREL O "Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department." Cyprus Journal of Medical Sciences, 4, ss.19 - 23, 2019.
ISNAD	Gökhan, Servan vd. "Importance of Thiol/Disulfide Homeostasis and the Neutrophil-to-Lymphocyte Ratio in Diagnosing of Urinary Tract Infections in the Emergency Department". Cyprus Journal of Medical Sciences 4/1 (2019), 19-23.