Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris
Yıl: 2020 Cilt: 4 Sayı: 1 Sayfa Aralığı: 16 - 21 Metin Dili: İngilizce DOI: 10.31594/commagene.704271 İndeks Tarihi: 14-09-2020
Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris
Öz: Cytokinins and auxins were studied in Lens culinaris before; however, there was lack of information about theireffects on vascular tissues and their relation with plant development. In this study, intact lentil seedlings were treated withdifferent doses of BAP (1 mg/L) and NAA (0.3, 0.6, 0.9, 1.2 mg/L) with periodic intervals of 7, 14, 21, 28, and 35 days under invitro conditions. The behaviors of plant height, number of shoots per plant, and rooting were investigated to find if theirregeneration behavior is related with the developmental behavior of phloem and xylem thickness and stem radius. In mostinstances, seven-day culture conditions in all plant growth regulators (PGRs) doses demonstrated the best results. BAP andNAA treatments with high doses on prolonged days of culture caused unhealthy growing on plant development and vasculartissues. While phloem region thickness was the highest in control group, xylem region was found to have the highest thicknessin 1 mg/L BAP treatment used singly. Moreover, high doses of BAP and NAA caused deformity and shrinking in phloem andxylem regions that also lead to shrinking in stem radius. These results reveal negative effects of BAP and NAA treatments onprolonged days of culture and conclude that the time period of treatment must be optimized precisely to avoid damages tovascular and other tissues and promote desirable growth and development of tissues.
Anahtar Kelime: Lens culinaris'te Sitokinin ve Oksinin Bitki Gelişimi ve Vasküler Dokular Üzerine Etkileri
Öz: Sitokininler ve oksinlerin Lens culinaris’te daha önce çalışılmasına rağmen bunların vasküler dokular ve bitki gelişimi üzerine olan etkileri hakkında bilgi bulunmamaktadır. Bu çalışmada, bütün mercimek bitkicikleri çeşitli BAP (1 mg/L) ve NAA dozlarında (0.3, 0.6, 0.9, 1.2 mg/L) periyodik 7, 14, 21, 28 ve 35 gün aralıklarında in vitro koşullarda kültüre alınmıştır. Bitki boyu, her bitkideki sürgün sayısı ve köklenmenin rejenerasyon davranışlarının; floem ve ksilem bölgesinin kalınlığının gelişimsel davranışı ve gövde çapının kalınlığı ile bir ilişkisinin olup olmadığı araştırılmıştır. Çoğu durumda, 7 günlük kültür koşulları tüm bitki büyüme düzenleyicileri dozlarında en iyi sonuçları göstermiştir. Yüksek dozdaki BAP ve NAA uygulamaları, uzatılan kültür günü sürelerinde bitki gelişimi ve vasküler dokular üzerinde sağlıksız gelişime neden olmuştur. Floem bölgesinin kalınlığı kontrol grubunda en yüksek olurken, ksilem bölgesinin yalnızca 1 mg/L BAP uygulamasında en yüksek kalınlığa sahip olduğu bulunmuştur. Ayrıca, yüksek BAP ve NAA dozları floem ve ksilem bölgesinde deformasyona ve daralmaya neden olmuştur ve gövde çapında daralmaya yol açmıştır. Bu sonuçlar, uzayan kültür günü sürelerindeki bitki büyüme düzenleyicileri uygulamalarının olumsuz etkilerini ortaya çıkarmaktadır. Vasküler dokular ve diğer dokulardaki hasarı azaltmak, dokulardaki istenen büyüme ve gelişmeyi elde etmek için gün periyodunun tam olarak optimize edilmesi gerektiği sonucuna varılmaktadır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | KÜPLEMEZ H, Yildirim M (2020). Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris. , 16 - 21. 10.31594/commagene.704271 |
| Chicago | KÜPLEMEZ Haydar,Yildirim Mehmet Ugur Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris. (2020): 16 - 21. 10.31594/commagene.704271 |
| MLA | KÜPLEMEZ Haydar,Yildirim Mehmet Ugur Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris. , 2020, ss.16 - 21. 10.31594/commagene.704271 |
| AMA | KÜPLEMEZ H,Yildirim M Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris. . 2020; 16 - 21. 10.31594/commagene.704271 |
| Vancouver | KÜPLEMEZ H,Yildirim M Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris. . 2020; 16 - 21. 10.31594/commagene.704271 |
| IEEE | KÜPLEMEZ H,Yildirim M "Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris." , ss.16 - 21, 2020. 10.31594/commagene.704271 |
| ISNAD | KÜPLEMEZ, Haydar - Yildirim, Mehmet Ugur. "Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris". (2020), 16-21. https://doi.org/10.31594/commagene.704271 |
| APA | KÜPLEMEZ H, Yildirim M (2020). Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris. Commagene Journal of Biology, 4(1), 16 - 21. 10.31594/commagene.704271 |
| Chicago | KÜPLEMEZ Haydar,Yildirim Mehmet Ugur Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris. Commagene Journal of Biology 4, no.1 (2020): 16 - 21. 10.31594/commagene.704271 |
| MLA | KÜPLEMEZ Haydar,Yildirim Mehmet Ugur Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris. Commagene Journal of Biology, vol.4, no.1, 2020, ss.16 - 21. 10.31594/commagene.704271 |
| AMA | KÜPLEMEZ H,Yildirim M Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris. Commagene Journal of Biology. 2020; 4(1): 16 - 21. 10.31594/commagene.704271 |
| Vancouver | KÜPLEMEZ H,Yildirim M Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris. Commagene Journal of Biology. 2020; 4(1): 16 - 21. 10.31594/commagene.704271 |
| IEEE | KÜPLEMEZ H,Yildirim M "Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris." Commagene Journal of Biology, 4, ss.16 - 21, 2020. 10.31594/commagene.704271 |
| ISNAD | KÜPLEMEZ, Haydar - Yildirim, Mehmet Ugur. "Effects of Cytokinin and Auxin on Plant Development and Vascular Tissues in Lens culinaris". Commagene Journal of Biology 4/1 (2020), 16-21. https://doi.org/10.31594/commagene.704271 |
