N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats
Yıl: 2020 Cilt: 30 Sayı: 1 Sayfa Aralığı: 60 - 68 Metin Dili: İngilizce DOI: 10.5137/1019-5149.JTN.26801-19.3 İndeks Tarihi: 11-11-2020
N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats
Öz: AIM: To investigate the neuroprotective effect of a N-methyl-D-aspartate (NMDA) receptor antagonist (amantadine) in an experimentalspinal cord injury (SCI) model.MATERIAL and METHODS: Thirty male Sprague–Dawley rats were divided into three groups: control (I), SCI (II), and SCI +amantadine (III). SCI was created using clip compression technique. At the end of day 7, blood samples were obtained from the ratsand analyzed using various biochemical markers. Histological examination was also performed. MDA, GSH, and MPO assays weredone. VEGF, TNF-α, and Baxexpressions were also analyzed.RESULTS: The group III had several inflammatory cells in the gray and white matter, with mildly degenerated multipolar and bipolarcells. Some bipolar and multipolar neurons showed TNF-α expression; however, TNF-α was found to be weak in small groupsof inflammatory cells around the blood vessels in the substantia grisea and alba. Positive Bax expression was observed in thesubstantia grisea layer, particularly in the membrane of some bipolar neurons and glial cells; however, negative Bax expression wasobserved in neuron and glial cells and showed positive VEGF expression in the vascular endothelium in the group III.CONCLUSION: NMDA receptor antagonists, especially amantadine, may ameliorate SCI by inducing angiogenesis, affectinginflammation and apoptosis. It inhibits oxidative stress and the signaling pathways following SCI in rats.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Aydoseli A, Can H, Aras Y, Sabanci PA, Akcakaya MO, Unal OF: Memantine and Q-VD-OPh treatments in experimental spinal cord injury: Combined inhibition of necrosis and apoptosis. Turk Neurosurg 26(5):783-789, 2016
- 2. Azbill RD, Mu X, Bruce-Keller AJ, Mattson MP, Springer JE: Impaired mitochondrial function, oxidative stress and altered antioxidant enzyme activities following traumatic spinal cord injury. Brain Research 765(2):283-290, 1997
- 3. Banik NL, Hogan EL, Whetstine LJ, Balentine JD: Changes in myelin and axonal proteins in Cacl2-induced myelopathy in rat spinal cord. Cent Nerv Syst Trauma 1:131-137, 1984
- 4. Baptiste DC, Fehlings MG: Pharmacological approaches to repair the injured spinal cord. Journal of Neurotrauma 23(3-4): 318-334, 2006
- 5. Baran O, Cicekci E, Donder A, Atic R, Deveci E, Tuncer MC: Protective effects of melatonin on spinal cord injury. Int J Morphol 36(2):488-492, 2018
- 6. Chronidou F, Apostolakis E, Papapostolou I, Grintzalis K, Georgiou, CD, Koletsis EN, Dougenis D: Beneficial effect of the oxygen free radical scavenger amifostine (WR-2721) on spinal cord ischemia/reperfusion injury in rabbits. Journal of Cardiothoracic Surgery 4(1):50, 2009
- 7. del Rayo Garrido M, Silva‐García R, García E, Martiñón S, Morales M, Mestre H, Ibarra A: Therapeutic window for combination therapy of A91 peptide and glutathione allows delayed treatment after spinal cord injury. Basic & Clinical Pharmacology & Toxicology 112(5):314-318, 2013
- 8. Dinomais M, Stana L, Egon G, Richard I, Menei P: Significant recovery of motor function in a patient with complete T7 paraplegia receiving etanercept. J Rehabil Med 41(4):286- 288, 2009
- 9. Dumont RJ, Okonkwo DO, Verma S, Hurlbert RJ, Boulos PT, Ellegala DB, Dumont AS: Acute spinal cord injury, part I: Pathophysiologic mechanisms. Clinical Neuropharmacology 24(5):254-264, 2001
- 10. Emmez H, Borcek AO, Kaymaz M, Kaymaz F, Durdag E, Civi S, Gulbahar O, Aykol S, Pasaoglu A: Neuroprotective effects of gabapentin in experimental spinal cord injury. World Neurosurg 73(6):729-734, 2010
- 11. Emmez H, Yildirim Z, Kale A, Tonge M, Durdag E, Borcek AO, Baykaner MK: Anti-apoptotic and neuroprotective effects of alpha-lipoic acid on spinal cord ischemia–reperfusion injury in rabbits. Acta Neurochir 152:1591-601, 2010
- 12. Esposito E, Cuzzocrea S: TNF-alpha as a therapeutic target in inflammatory diseases, ischemia-reperfusion injury and trauma. Curr Med Chem 16(24):3152-3167, 2009
- 13. Fan L, Wang K, Cheng B: Effects of buyang huanwu decoction on apoptosis of nervous cells and expressions of Bcl-2 and bax in the spinal cord of ischemia-reperfusion injury in rabbits. J Tradit Chin Med 26(2):153-156, 2006
- 14. Happel RD, Smith KP, Banik NL, Powers JM, Hogan EL, Balentine JD: Ca2+- accumulation in experimental spinal cord tauma. Brain Res 211:476-479, 1981
- 15. Herrera JJ, Nesic O, Narayana PA: Reduced vascular endothelial growth factor expression in contusive spinal cord injury. J Neurotrauma 26(7):995-1003, 2009
- 16. Hillegass LM, Hillegass LM, Griswold DE, Brickson B, Albrightson-Winslow C: Assessment of myeloperoxidase activity in whole rat kidney. J Pharmacol Methods 24(4):285- 295, 1990
- 17. Jiang ZS, Pu ZC, Hao ZH: Carvacrol protects against spinal cord injury in rats via suppressing oxidative stress and the endothelial nitric oxide synthase pathway. Molecular Medicine Reports 12(4): 5349-5354, 2015
- 18. Kale A, Borcek AO, Emmez H, Yildirim Z, Durdag E, Lortlar N, Kilic N: Neuroprotective effects of gabapentin on spinal cord ischemia-reperfusion injury in rabbits. J Neurosurg Spine 15(3):228-237, 2011
- 19. Kochhar A, Zivin JA, Lyden PD, Mazzarella V: Glutamate antagonist therapy reduces neurologic deficits produced by focal central nervous system ischemia. Arch Neurol 45:148– 153, 1988
- 20. Kotipatruni RR, Dasari VR, Veeravalli KK, Dinh DH, Fassett D, Rao JS: p53-and Bax-mediated apoptosis in injured rat spinal cord. Neurochemical research 36(11): 2063, 2011
- 21. Kwon BK, Okon E, Hillyer J, Mann C, Baptiste D, Weaver LC, Tetzlaff W: A systematic review of non-invasive pharmacologic neuroprotective treatments for acute spinal cord injury. J Neurotrauma 28(8):1545-1588, 2011
- 22. Kwon BK, Tetzlaff W, Grauer JN, Beiner J, Vaccaro AR: Pathophysiology and pharmacologic treatment of acute spinal cord injury. The Spine Journal 4(4):451-464, 2004
- 23. Moonen G, Satkunendrarajah K, Wilcox JT, Badner A, Mothe A, Foltz W, Fehlings MG, Tator CH: A new acute impactcompression lumbar spinal cord injury model in the rodent. Journal of Neurotrauma 33(3):278-289, 2016
- 24. Pristupa ZB, McConkey F, Liu F, Man HY, Lee FJ, Wang YT, Niznik HB: Protein kinase-mediated bidirectional trafficking and functional regulation of the human dopamine transporter. Synapse 30(1):79-87, 1998
- 25. Qian H, Liu D: The time course of malondialdehyde production following impact injury to rat spinal cord as measured by microdialysis and high pressure liquid chromatography. Neurochem Res 22:1231-1236, 1997
- 26. Qin ZH, Chen RW, Wang Y, Nakai M, Chuang DM, Chase TN: Nuclear factor kappaB nuclear translocation upregulates c-Myc and p53 expression during NMDA receptor-mediated apoptosis in rat striatum. J Neurosci 19(10):4023-4033, 1999
- 27. Rivlin AS, Tator CH: Effect of duration of acute spinal cord compression in a new acute cord injury model in rat. Surg Neurol 9:39-43, 1978
- 28. Rokkas CK, Rokkas CK, Helfrich Jr LR, Lobner DC, Choi DW, Kouchoukos NT: Dextrorphan inhibits the release of excitatory amino acids during spinal cord ischemia. Ann Thorac Surg 58: 312-319, 1994
- 29. Shacka JJ, Roth KA: Regulation of neuronal cell death and neurodegeneration by members of the Bcl-2 family: Therapeutic implications. Curr Drug Targets CNS Neurol Disord 4(1):25-39, 2005
- 30. Vanle B, Olcott W, Jimenez J, Bashmi L, Danovitch I, Ishak WW: NMDA antagonists for treating the non-motor symptoms in Parkinson’s disease. Translational Psychiatry 8(1):117, 2018
- 31. Wang T, Huang XJ, Van KC, Went GT, Nguyen JT, Lyeth BG: Amantadine improves cognitive outcome and increases neuronal survival after fluid percussion traumatic brain injury in rats. Journal of Neurotrauma 31(4):370-377, 2014
- 32. Wilson RB, Eliyan Y, Sankar R, Hussain SA: Amantadine: A new treatment for refractory electrical status epilepticus in sleep. Epilepsy & Behavior 84:74-78, 2018
| APA | DOĞAN G, Karaca O (2020). N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats. , 60 - 68. 10.5137/1019-5149.JTN.26801-19.3 |
| Chicago | DOĞAN Güvenç,Karaca Onur N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats. (2020): 60 - 68. 10.5137/1019-5149.JTN.26801-19.3 |
| MLA | DOĞAN Güvenç,Karaca Onur N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats. , 2020, ss.60 - 68. 10.5137/1019-5149.JTN.26801-19.3 |
| AMA | DOĞAN G,Karaca O N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats. . 2020; 60 - 68. 10.5137/1019-5149.JTN.26801-19.3 |
| Vancouver | DOĞAN G,Karaca O N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats. . 2020; 60 - 68. 10.5137/1019-5149.JTN.26801-19.3 |
| IEEE | DOĞAN G,Karaca O "N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats." , ss.60 - 68, 2020. 10.5137/1019-5149.JTN.26801-19.3 |
| ISNAD | DOĞAN, Güvenç - Karaca, Onur. "N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats". (2020), 60-68. https://doi.org/10.5137/1019-5149.JTN.26801-19.3 |
| APA | DOĞAN G, Karaca O (2020). N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats. Turkish Neurosurgery, 30(1), 60 - 68. 10.5137/1019-5149.JTN.26801-19.3 |
| Chicago | DOĞAN Güvenç,Karaca Onur N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats. Turkish Neurosurgery 30, no.1 (2020): 60 - 68. 10.5137/1019-5149.JTN.26801-19.3 |
| MLA | DOĞAN Güvenç,Karaca Onur N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats. Turkish Neurosurgery, vol.30, no.1, 2020, ss.60 - 68. 10.5137/1019-5149.JTN.26801-19.3 |
| AMA | DOĞAN G,Karaca O N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats. Turkish Neurosurgery. 2020; 30(1): 60 - 68. 10.5137/1019-5149.JTN.26801-19.3 |
| Vancouver | DOĞAN G,Karaca O N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats. Turkish Neurosurgery. 2020; 30(1): 60 - 68. 10.5137/1019-5149.JTN.26801-19.3 |
| IEEE | DOĞAN G,Karaca O "N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats." Turkish Neurosurgery, 30, ss.60 - 68, 2020. 10.5137/1019-5149.JTN.26801-19.3 |
| ISNAD | DOĞAN, Güvenç - Karaca, Onur. "N-methyl-D-aspartate Receptor Antagonists may Ameliorate Spinal Cord Injury by Inhibiting Oxidative Stress: An Experimental Study in Rats". Turkish Neurosurgery 30/1 (2020), 60-68. https://doi.org/10.5137/1019-5149.JTN.26801-19.3 |
