Yıl: 2020 Cilt: 4 Sayı: 1 Sayfa Aralığı: 70 - 89 Metin Dili: İngilizce DOI: 10.31015/jaefs.2020.9 İndeks Tarihi: 03-10-2020

Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization

Öz:
Fatty acid determination is used for the characterization of the lipid fraction in foods, providing essential informationregarding feed and food quality. Most edible fats and oils are composed primarily of linear saturated fatty acids,branched, mono-unsaturated, di-unsaturated, and higher unsaturated fatty acids. To attain this information we developed a gas chromatography (GC) method that can separate fatty acids from C4 to C24 using mass spectrometry identification. A simplified sample preparation procedure was applied so it is not time-consuming and short enough to avoidfat degradation. Additionally, one-step derivatization was applied to obtained fatty acid methyl esters in situ in the gaschromatograph injection port, using tetramethylammonium hydroxide and a high polarity polyethylene glycol-basedcross-linked microbore chromatographic column was coupled to achieve the separation of 60 compounds in under 15minutes with extreme sensibility. The versatility of the method allows fatty acid profile (including saturated [SFA],monounsaturated [MUFA], and polyunsaturated fatty acids [PUFA]) information to be gathered in different productsof primary production i. raw materials commonly used in the production of animal feed, ii. profiles for balancedfeed for laying hens, beef cattle and dairy cattle and iii. products of animal origin intended for human consumption,such as meat, eggs, and milk. Our data (performance parameters and fatty acid profiles) support the validity of theresults; the method can be used for quality assurance both in productive species feed and feed ingredients, pet food,and related food matrices. The technique presented herein can be used as a high-throughput routine screening toolto assess fat quality as this data is paramount to improve animal nutrition and health and animal-derived products ofhuman consumption.
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APA LEİVA A, CHİNCHİLLA F (2020). Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization. , 70 - 89. 10.31015/jaefs.2020.9
Chicago LEİVA Astrid,CHİNCHİLLA Fabio Granados Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization. (2020): 70 - 89. 10.31015/jaefs.2020.9
MLA LEİVA Astrid,CHİNCHİLLA Fabio Granados Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization. , 2020, ss.70 - 89. 10.31015/jaefs.2020.9
AMA LEİVA A,CHİNCHİLLA F Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization. . 2020; 70 - 89. 10.31015/jaefs.2020.9
Vancouver LEİVA A,CHİNCHİLLA F Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization. . 2020; 70 - 89. 10.31015/jaefs.2020.9
IEEE LEİVA A,CHİNCHİLLA F "Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization." , ss.70 - 89, 2020. 10.31015/jaefs.2020.9
ISNAD LEİVA, Astrid - CHİNCHİLLA, Fabio Granados. "Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization". (2020), 70-89. https://doi.org/10.31015/jaefs.2020.9
APA LEİVA A, CHİNCHİLLA F (2020). Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization. International Journal of Agriculture, Environment and Food Sciences, 4(1), 70 - 89. 10.31015/jaefs.2020.9
Chicago LEİVA Astrid,CHİNCHİLLA Fabio Granados Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization. International Journal of Agriculture, Environment and Food Sciences 4, no.1 (2020): 70 - 89. 10.31015/jaefs.2020.9
MLA LEİVA Astrid,CHİNCHİLLA Fabio Granados Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization. International Journal of Agriculture, Environment and Food Sciences, vol.4, no.1, 2020, ss.70 - 89. 10.31015/jaefs.2020.9
AMA LEİVA A,CHİNCHİLLA F Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization. International Journal of Agriculture, Environment and Food Sciences. 2020; 4(1): 70 - 89. 10.31015/jaefs.2020.9
Vancouver LEİVA A,CHİNCHİLLA F Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization. International Journal of Agriculture, Environment and Food Sciences. 2020; 4(1): 70 - 89. 10.31015/jaefs.2020.9
IEEE LEİVA A,CHİNCHİLLA F "Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization." International Journal of Agriculture, Environment and Food Sciences, 4, ss.70 - 89, 2020. 10.31015/jaefs.2020.9
ISNAD LEİVA, Astrid - CHİNCHİLLA, Fabio Granados. "Fatty acid profiling in animal feeds and related food matrixes using a fast GC/MS method and in situ derivatization". International Journal of Agriculture, Environment and Food Sciences 4/1 (2020), 70-89. https://doi.org/10.31015/jaefs.2020.9