Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods

şensoy, erhan; ÖZNURLU, Yasemin

doi:10.5152/tjg.2019.18681

Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods

Erhan ŞENSOY, (Karamanoğlu Mehmetbey Üniversitesi, Sağlık Bilimleri Yüksekokulu, Ebelik Bölümü, Karaman, Türkiye)

Yasemin ÖZNURLU (Selçuk Üniversitesi, Veteriner Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Konya, Türkiye)

Turkish Journal of Gastroenterology

1 1

Yıl: 2019 Cilt: 30 Sayı: 10 Sayfa Aralığı: 917 - 924 Metin Dili: İngilizce DOI: 10.5152/tjg.2019.18681 İndeks Tarihi: 16-07-2020

Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods

Öz:

Background/Aims: The aim of the present study was to determine the changes on the small intestine in mice during pregnancy usinghistological, enzyme histochemical, and immunohistochemical methods.Materials and Methods: A total of 24 Swiss albino female mice were divided as non-pregnant/control, first week, second week, and thirdweek of pregnancy (n=6). Tissue samples obtained from the duodenum, jejunum, and ileum were processed by means of routine histological techniques and stained with Crossmon’s triple staining. Alkaline phosphatase (ALP) was demonstrated with the simultaneousazo-coupling method. Proliferating cell nuclear antigen (PCNA) was demonstrated with the streptavidin-biotin-peroxidase complexmethod. The numerical data of the parameters were obtained and analyzed statistically.Results: Villus height, villus width, and the rate of villus height/crypt depth were decreased in the duodenum, jejunum, and ileum in thelast week of pregnancy compared with the control group. Changes in the crypt depth of the duodenum, jejunum, and ileum in pregnancywere found. The muscle width increased in pregnancy. It was identified that the ALP reactivity statistically significantly increased in theduodenum, jejunum, and ileum in pregnancy. The percentage of PCNA-positive cells in the duodenum, jejunum, and ileum increased inthe first and second weeks of pregnancy, whereas it decreased in the third week of pregnancy compared with non-pregnant controlanimals.Conclusion: In conclusion, villus parameters, ALP reactivity, and percentage of PCNA-positive cells in the small intestine were affectedduring pregnancy.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Ch’ng CL, Morgan M, Hainsworth I, Kingham JG. Prospective study of liver dysfunction in pregnancy in Southwest Wales. Gut 2002; 51: 876-80. [CrossRef]
2. Nalbant S. Gebelikte gelişen fizyolojik değişiklikler. Onuncu Ulusal İç Hastalıkları Kongresi Antalya 2008; 39-40.
3. Chung SY, Ravel J, Regan M. Clinical relevance of gastrointestinal microbiota during pregnancy: A primer for nurses. Biol Res Nurs 2018; 20: 84-102. [CrossRef]
4. Sur E, Oznurlu Y, Ozaydın T. The determination of Ghrelin immunoreactivity in gastric mucosa of fundus during pregnancy in mice. Int J Biomed Res 2017; 8: 672-6.
5. Casirola DM, Ferraris RP. Role of the small intestine in postpartum weight retention in mice. Am J Clin Nutr 2003; 78: 1178-87. [CrossRef]
6. Meyer AM, Caton JS. Role of the small ıntestine in developmental programming: Impact of maternal nutrition on the dam and offspring. Adv Nutr January 2016; 7: 169-78. [CrossRef]
7. Kumar V, Gill KD. To estimate the activity of alkaline phosphatase in serum in basic concepts in clinical biochemistry: A practical guide. Springer Singapore 2018; 107-9. [CrossRef]
8. Sakharov IYU, Makasova I, Ermolin GA. Purification and characterisation of intestinal alkaline phosphatase from harp seal comp. Biochem Physiol 1988; 90: 709-14. [CrossRef]
9. Klumpp S, Schultz JE. Alkaline phosphatase from pramercium cilia and cell bodies purification and characterisation. Biochem Biophys Acta 1990; 1037: 233-9. [CrossRef]
10. Kumandaş S, Kurtoğlu S. Çocukluk döneminde alkalen fosfataz enziminin değerlendirilmesi. Yeni Tıp Drg 1992; 9: 68-70.
11. Kaplan LA, Pesce AJ. Clinical chemistry theory analysis and correlation. Mosby Third Edition. Baltimore Boston Chicago Newyork London Madrid Mexico City Singapore Tokyo Toronto 1996; 514-5.
12. Uzunoğlu N. Alkalen fosfataz enziminin fizikokimyasal özellikleri. T Klin J Med Sci 1998; 18: 69-75.
13. Uysal O; İnsan plasental alkalen fosfatazın histokimyasal lokalizasyonu. Ankara Ün Dikimevi MYO Drg 2003; 4: 13-21. [CrossRef]
14. Hamarneh SR, Kim BM, Kaliannan K, Morrison SA. Intestinal alkaline phosphatase attenuates alcohol-induced hepatosteatosis in mice. Dig Dis Sci 2017; 62: 2021-34. [CrossRef]
15. Gu X, Li D, She R. Effect of weaning on small intestinal structure and function in the piglet. Arch Anim Nutr 2002; 56: 275-86. [CrossRef]
16. Özaydın T. Kuluçkada deneysel olarak oluşturulan ısı stresinin boylerlerde ince bağırsağın embriyonik gelişimi üzerindeki etkilerinin histokimyasal, immünohistokimyasal ve histometrik metotlarla belirlenmesi. Selçuk Ün Sağlık Bil Ens Dr Tezi Konya 2009.
17. Posen S. Alkaline phosphate. Ann Int Med 1967; 67: 183-203. [CrossRef]
18. Shane JM, Suzuki K. Placenta alkaline phosphate. A review and re-evaluation of its applicability in monitoring fetoplacental function. Obst Gynec Survey 1974; 29: 97-105. [CrossRef]
19. Ishıhara N, Matsuo H, Murakoshı H, Laoag-Fernandez J, Samoto T, Maruo T. Changes in proliferative potential, apoptosis and Bcl-2 protein expression in cytotrophoblasts and syncytiotrophoblast in human placenta over the course of pregnancy. Endocr J 2000; 47: 317-27. [CrossRef]
20. Okamoto T, Seo H, Mano H, et al. Expression of human placental alkaline phosphatase in placenta during pregnanc. Placenta 1990; 2: 319-27. [CrossRef]
21. Levine B. The early trophoblast a review including theoretical considerations. Obst Gynec 1961; 17: 769-78.
22. Jeacock MK, Monis NF, Plester JA. The activity of alkaline and acid phospatase in the human placenta. J Obst Gynaeo Brit 1963; 70: 267-73. [CrossRef]
23. Dong Y, Liu G, Wang Z, Li J, Cao J, Chen Y. Effects of catecholaminergic nerve lesion on endometrial development during early pregnancy in mice. Histol Histopathol 2015; 11: 1-23.
24. Hall P, Levison D, Woods A. Proliferating cell nuclear antigen PCNA immunolocalization in parafin sections an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol 1990; 62: 285-94. [CrossRef]
25. Soyuer I, Canöz Ö, Er Ö, Deniz K, Soyuer S. Malign mezotelyomada (proliferating nuclear cell antijen) PCNA immünreaktivitesinin ve mitotik indeksin prognoza etkisi. Erciyes Ün Tıp Fk Drg 2002; 24: 115-9.
26. Maga G, Hübscher U. Proliferating cell nuclear antigen-PCNA a dancer with many partners. Journ of Cell Sci 2003; 116: 3051-60. [CrossRef]
27. Kelman Z. PCNA: Structure, function and interactions. Oncogene 1997; 14: 629-40. [CrossRef]
28. Foley JF, Dietrich DR, Swenberg JA, Maronpot RR. Detection and evaluation of proliferating cell nuclear antigen PCNA in rat tissue by an improved immunohistochemical procedure. J Histotech 1991; 14: 237-41. [CrossRef]
29. Şen O, Kayaselçuk F, Zorludemir S, Aydın MV, Erdogan B. Meningiomlarda histopatolojik tanının flovsitometrik DNA analizi, PCNA ve KI-67 ile korelasyonu. Türk Nöroşürurji Drg 2002; 12: 48-53.
30. Özaydın T, Çelik İ. Histological, histochemical and immunohistochemical investigations on the developing small intestines of broiler embryos. Journ of Ani and Vet Adv 2012; 11: 2936-44. [CrossRef]
31. Banlunara W, Bintvihok A,Kumagai S, Immunohistochemical study of proliferating cell nuclear antigen (PCNA) in duckling liver fed with aflatoxin B1 and esterified glucomannan. Toxicon 2005; 46: 954-7. [CrossRef]
32. Stoimenov I, Helleday T. Atlas of genetics and cytogenetics in oncology and haematology. Department of Genetics Microbiology, Toxicology, Stockholm University, Stockholm, Sweden (IS, TH); Gray Institute for Radiation Oncology & Biology, University of Oxford, Oxford 2011, 106 91.
33. Sones JL, Davisson RL. Preeclampsia, of mice and women. Physiol Genomics 2016; 48: 565-72. [CrossRef]
34. Culling FA, Allison RT, Barr WT. Cellular pathology technique. Butterworts and Co Ltd London, 1985; 25-9. [CrossRef]
35. Lojda Z, Grossrau R, Schibler TH. Enzyme histochemistry. Springer-Verlag Berlin, 1979; 59-70. [CrossRef]
36. Uni Z, Geyra A, Ben-Hur H, Sklan D. Small intestinal development in the young chick crypt formation and enterocyte proliferation and migration. Br Poult Sci 2000; 41: 544-51. [CrossRef]
37. Mohammed SA, Ibrahim SH. Histological and immunohistochemical study on the effect of pregnancy and lactation on the jejunal mucosa of albino rat. J Egypt Hist 2004; 27: 375-88.
38. Van Dyke RW. The liver in pregnancy. In: Boyer TD, Wright Tl (eds). Zakim and Boyer’s Hepatology: A textbook of liver disease. 5th ed. USA: Sounders Elseiver, 2006; 1003-29. [CrossRef]
39. Solomon EP, Berg LR, Martin DW. Biology. Saunders Collage Publishing, electronic resource 9th ed. Belmont. CA Brooks/Cole. 2011; 32: 48-52.
40. Erhan F, Ergün L. Kanatlı ve memeli karaciğerinde karbonhidrat ve yağ metabolizmasının karşılaştırılması. MAKÜ Sağ Bil Enst Derg 2018; 6: 33-42. [CrossRef]
41. Collado MC, Isolauri E, Laitinen K, Salminen S. Distinct composition of gut microbiota during pregnancy in overweight and normal weight women. Am J Clin Nutr 2008; 88: 894-9. [CrossRef]
42. Gohir W, Whelan FJ, Surette MG, Moore C, Jonathan D. Pregnancy-related changes in the maternal gut microbiota are dependent upon the mother’s periconceptional diet. Gut Microbes 2015; 6: 310- 20. [CrossRef]
43. Güven S, Türkay C. Gebelik ve karaciğer hastalıkları. Güncel Gastroenteroloji, 2011; 15: 107-13.
44. Cripps AW, Williams VJ. The effect of pregnancy and lactation on food intake, gastrointestinal anatomy and the absorptive capacity of the small intestine in the albino rat. Br J Nutr 1975; 33: 17-32. [CrossRef]
45. Williamson DH. Regulation of metabolism during lactation in the rat. Reprod Nutr Develop 1986; 26: 597-603. [CrossRef]
46. Boass A, Lovdal JA, Toverud SU. Pregnancy-and lactation-induced changes in active intestinal calcium transport in rats. Am J Gastrointest Liver Physiol 1992; 263: 127-34. [CrossRef]
47. Burdett K, Reek C. Adaptation of the small intestine during pregnancy and lactation in the rat. Biochem J 1979; 184: 245-51. [CrossRef]
48. Clarke RM. The effects of age on mucosal morphology and epithelial cell production in rat small intestine. J Anat 1977; 123: 805-11.
49. Harmatz P, Carrington P, Barry V, Hatz R, Bloch K. Intestinal adaptation during lactation in the mouse altered intestinal processing of a dietary protein. Am J Phys 1993; 264: 1126-34. [CrossRef]
50. Cairnie AB, Bentley RE. Cell proliferation studies in the intestinal epithelium of the rat: Hyperplasia during lactation. Exp Cell Res 1967; 46: 428-40. [CrossRef]
51. Firmansyah A, Suwandito L, Penn D, Lebenthal E. Biochemical and morphological changes in the digestive tract of rats after prenatal and postnatal malnutrition. Am J Clin Nutr 1989; 50: 216-8. [CrossRef]
52. Folley SJ, Greenbaum AL. Changes in the arginase and alkaline phosphatase contents of the mammary gland and liver of the rat during pregnancy, lactation and mammary involution. Nat Inst for Res in Dairying 1946; 41: 261-8. [CrossRef]
53. Manning JP, Meli A, Steinetz BG. Alkaline phosphatase and β-glucuronidase activity in the rat uterus during early pregnancy. J Endocrinol 1966; 35: 385-91. [CrossRef]
54. Murdoch RN, Kay DJ, Cross M. Activity and subcellular distribution of mouse uterine alkaline phosphatase during pregnancy and pseudopregnancy. J Reprod Fert 1978; 54: 293-300. [CrossRef]
55. Valenzuela GJ, Munson LA, Tarbaux NM, Farley JR. Time-dependent changes in bone, placental, intestinal, and hepatic alkaline phosphatase activities in serum during human pregnancy. Clin Chem 1987; 33: 1801-6.
56. Maruo T, Ishihara N, Samoto T, Murakoshi H, Laoag-Fernandez JB, Matsuo H. Regulation of human trophoblast proliferation and apoptosis during pregnancy. Early Pregn 2001; 5: 28-9.
57. Elpek GÖ, Karaveli Ş, Keleş N. Preeklampsili olguların term plasentalarında villöz trofoblast preliferasyonunun incelenmesi. Türk Pathol Derg 2000; 16: 10-2.
58. Erboga M, Kanter M. Trophoblast cell proliferation and apoptosis in placental development during early gestation period in rats. Anal Quant Cytopathol Histpathol 2015; 37: 286-94.
59. Ozaydın T, Sur E, Oznurlu Y, Celik I, Uluisik D. Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages. Microsc Res Tech 2016; 79: 251-7. [CrossRef]
60. Oner H, Oner J, Demir R. Distributions of PCNA and Cas-3 in rat uterus during early pregnancy. Folia Histochem Cytobiol 2010; 48: 71-7. [CrossRef]
61. Danihel L, Gomolcak P, Korbel M, et al. Expression of proliferation and apoptotic markers in human placenta during pregnancy. Acta Histochem 2002; 104: 335-8. [CrossRef]
62. Acar N, Korgun ET, Cayli S, Sahin Z, Demir R, Ustunel I. Is there a relationship between PCNA expression and diabetic placental development during pregnancy? Acta Histochem 2008; 110: 408-17. [CrossRef]
63. Ozaydın T, Sur E, Oznurlu Y, Celık I., Uluısık D. Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages. Microsc Res Tech 2016; 79: 251-7. [CrossRef]

APA	şensoy e, ÖZNURLU Y (2019). Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods. , 917 - 924. 10.5152/tjg.2019.18681
Chicago	şensoy erhan,ÖZNURLU Yasemin Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods. (2019): 917 - 924. 10.5152/tjg.2019.18681
MLA	şensoy erhan,ÖZNURLU Yasemin Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods. , 2019, ss.917 - 924. 10.5152/tjg.2019.18681
AMA	şensoy e,ÖZNURLU Y Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods. . 2019; 917 - 924. 10.5152/tjg.2019.18681
Vancouver	şensoy e,ÖZNURLU Y Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods. . 2019; 917 - 924. 10.5152/tjg.2019.18681
IEEE	şensoy e,ÖZNURLU Y "Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods." , ss.917 - 924, 2019. 10.5152/tjg.2019.18681
ISNAD	şensoy, erhan - ÖZNURLU, Yasemin. "Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods". (2019), 917-924. https://doi.org/10.5152/tjg.2019.18681

APA	şensoy e, ÖZNURLU Y (2019). Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods. Turkish Journal of Gastroenterology, 30(10), 917 - 924. 10.5152/tjg.2019.18681
Chicago	şensoy erhan,ÖZNURLU Yasemin Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods. Turkish Journal of Gastroenterology 30, no.10 (2019): 917 - 924. 10.5152/tjg.2019.18681
MLA	şensoy erhan,ÖZNURLU Yasemin Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods. Turkish Journal of Gastroenterology, vol.30, no.10, 2019, ss.917 - 924. 10.5152/tjg.2019.18681
AMA	şensoy e,ÖZNURLU Y Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods. Turkish Journal of Gastroenterology. 2019; 30(10): 917 - 924. 10.5152/tjg.2019.18681
Vancouver	şensoy e,ÖZNURLU Y Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods. Turkish Journal of Gastroenterology. 2019; 30(10): 917 - 924. 10.5152/tjg.2019.18681
IEEE	şensoy e,ÖZNURLU Y "Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods." Turkish Journal of Gastroenterology, 30, ss.917 - 924, 2019. 10.5152/tjg.2019.18681
ISNAD	şensoy, erhan - ÖZNURLU, Yasemin. "Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods". Turkish Journal of Gastroenterology 30/10 (2019), 917-924. https://doi.org/10.5152/tjg.2019.18681